Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"liver development"”.
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Characterization of foetal hepatic cells during rat liver development
Die Leber entwickelt sich als Leberknospe aus dem Entoderm des unteren Abschnittes des Vorderdarmes. Die Leberknospe differenziert sich in 2 Teile: In den Leberteil und in den Galleteil. Während der embryonalen Entwicklung differenzieren sich die entodermalen Zellen zu Hepatoblasten, die sich zu …
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Mapping Human Liver Development using Single-Cell Transcriptomics to Reveal Pathways Directing Stem Cell Fates
The liver is an organ with a diversity of essential functions including xenobiotic removal, bile acid production, storage of iron and vitamins, and metabolism of glucose and fatty acids. Hepatocytes, comprising the liver parenchyma, fulfil these functions and form an extensive network with …
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The developmental liver transcriptome of Rattus norvegicus
… in the transcriptional dynamics of perinatal liver development. The transcription factors and their downstream effects have been well studied in regards to early liver development, yet perinatal liver development is much less well understood. In an attempt to investigate this area of research, …
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Systems Engineering of Stem Cell Fate
… cells have raised hope for cell therapy to treat liver ailments. They have enhanced the prospects of developing reliable in vitro models for liver diseases and drug toxicity screening. A differentiation protocol mimicking key signaling cues of embryonic development was developed to direct stem …
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Exogenous donor-derived hepatogenesis in chimeric hosts
… of intra- and interspecies chimeras to explore liver development and the potential for generating donor-derived organs in chimeric hosts. In this dissertation, we begin with a comprehensive review of the literature (Chapter I) regarding histology and anatomy of liver, cardinal functions of the …
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A THREE DIMENSIONAL MODEL OF HUMAN HEPATO-BILIARY ORGANOGENESIS
… have emerged aimed at generating functional liver tissue by using decellularized liver scaffolds repopulated with liver cells. The quest continues for identifying the best cell source for liver tissue engineering, but one cell source in particular, human fetal liver progenitor cells (hFLPCs), …
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Generation and characterisation of primary and pluripotent stem cell-derived human hepatoblast organoids
… that can perform only some functions of adult liver cells. To optimise such protocols, it is imperative to understand the signalling pathways directing liver development. However, such knowledge is difficult to capture especially in human for obvious ethical reasons. As an alternative to …
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Engineered microenvironments for studying liver progenitor differentiation
The bipotential differentiation of liver progenitor cells underlies liver development and bile duct formation as well as liver regeneration and disease. Both TGFβ and Notch signaling are known to play important roles in the liver progenitor specification process and subsequent tissue morphogenesis. …
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Genetic Analysis of The Hippo Pathway In Mouse Liver
… of these components, predominantly in the liver. A further liver specific analysis of the deletion mutation of these components, as well as the <em>Yap/Taz</em> double deletion mutation, reveals essential roles of the Hippo pathway in regulating hepatic quiescence and embryonic liver …
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Identification of a conserved alternative mRNA splicing program that supports hepatic growth and maturation during development and regeneration
… role in the brain, heart and skeletal muscle development; however, regulation of splicing or its function in hepatic growth and maturation is not well understood. The mammalian liver is a major metabolic organ responsible for a variety of functions and has an exceptional capacity for …
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Investigating the role of geometry, mechanics, and microenvironmental cues on liver progenitor cell differentiation
… how mechanical characteristics impact liver progenitor cell differentiation. In this dissertation, I will introduce and review relevant concepts of mechanobiology and the 2D and 3D in vitro systems used doe measurement and manipulation. I then describe my work combining microarrays with …
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The Role of Biomechanics in Liver Tissue Engineering
The field of liver bioengineering aims to generate functioning liver tissue in the laboratory in order to study complicated processes such as development, disease, cancer, drug metabolism, or even to engineer organs that can be implanted in patients. One approach in liver tissue engineering is to …
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Engineering Organoids for Stem Cell Maturation
The liver is the largest internal organ in the body. It is responsible for performing an array of vital functions, including the filtration of blood, synthesis of different molecules, and metabolism of drugs and toxicants. Hepatocytes, or the main liver cell type, perform most of these functions. …
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Production of hepatocytes from human pluripotent stem cells for cell-based therapy
… (HLCs) represent a promising alternative to liver transplantation. However, there are two major limitations that must be overcome before hPSCs derived hepatocytes can go into clinical trials. Firstly, current protocols are not compatible with Good Manufacturing Practice (GMP) as they rely on …
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A Systems Approach to Studying the Differentiation of Stem Cells Towards Hepatocytes
… to mimic the key signaling cues during embryonic development. However, most directed differentiation processes have run into issues with limited scalability and lack of functionality in the differentiated cells. In an effort to bring stem cell therapy closer to reality, our strategy was to use a …
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Towards an understanding of seasonal ecophysiology in north-temperate fish
… reproductive process. Here, independently cued development of the liver mediated observed reproductive allocation. Earlier warming interrupted liver development and the transfer of hepatic material and energy to the gonad (termed the “hepato-gonadal shunt”), a process putatively present in 20 …
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Charakterisierung der Selenoproteine Thioredoxinreduktase 1 und 2 anhand von Knock-out-Mausmodellen
… Total deficiency of TR1 leads to strong developmental retardation and malformations in organogenesis mostly affecting the turning of the embryo, closure of neural tube, formation of head structures and formation of somites. Thereby TR1 deficient embryos maximally reach the developmental …
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Early liver formation in zebrafish: a molecular and morphological approach
The digestive tract and its accessory organs - liver, pancreas, and the inner lining of the swim bladder - are derived from the endodermal germ layer. In zebrafish the digestive system starts out as a rod at the embryonic midline, which subsequently becomes patterned and outgrowth of its associated …
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Creation of organoid systems to model human endodermal organ development in vitro
… to use the technology to study human organ development. The work of this thesis established two novel hepatic organoid systems; one based upon the archetypal liver stem cell, the hepatoblast, whilst the other was based upon a hitherto undescribed biliary progenitor cell. The work continued …
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The role of the mechanical microenvironment in regulation of cholangiocyte specification and tubulogenesis
… fate and functionality associated with ageing, development and disease (Segel et al. 2019; Bansaccal et al. 2023). Despite most studies emphasising ECM stiffness, recent insights suggest a cooperative role of ECM ligand composition and stiffness in driving biological function. However, a major …
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