Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"live-cell microscopy"”.
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Regulation of the spindle checkpoint by Mad2 binding proteins
… the role of CMT2 in mitosis by fixed- and live-cell microscopy. CMT2 localizes to kinetochores in a Mad2-dependent manner and forms ternary complexes with Madl-Mad2 and Cdc20-Mad2 in vivo. Surprisingly, CMT2 is required for completion of mitosis even in the absence of spindle damage.
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Static and Microfluidic Live Imaging Studies of Plasmodium falciparum Invasion Phenotypes
… malaria. The merozoite, first invade a red blood cell (RBC) in the circulation, then grows, develops and multiplies within it by clonal division. Merozoite invasion is a complex process involving dynamic interactions between ligands in the merozoite coat and receptors on the red blood cell …
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Regulation of Morphogenetic Events in Saccharomyces cerevisiae
… polarized secretion and modification of the cell wall at the growing tip. The genetic requirements for initiating polarized growth are perhaps best understood for the model budding yeast, Saccharomyces cerevisiae. Once the cell is committed to enter the cell cycle by activation of G1 …
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Cargo Specific Regulation Of Cytoplasmic Dynein By Effector Proteins
… of cargo and organelles from the axon to the cell body is driven almost completely by the molecular motor, cytoplasmic dynein. Yet, it remains unclear how dynein is spatially and temporally regulated given the variety of cargo that must be properly localized to maintain cellular function. …
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Molecular and cellular analyses of pathogenicity and host specificity in rice blast disease
… and the plant. We have applied molecular and cellular analyses to understand critical processes in the M. oryzae disease cycle. The dark melanin pigment produced by the fungus is critical for the function of its specialized appressorial cell, which punches the leaf surface by generating the …
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Colony Stimulating Factor-1 Receptor (CSF-1R) Expression, Degradation, and Signaling in Lipid-Laden Macrophages
… in CSF-1 signaling and related pathways in foam cells remain poorly investigated. Lipid-laden macrophages were formed through exposure of murine bone marrow derived macrophages to 100 μg/ml acetylated LDL for 24 h. Non-LDL treated macrophages were grown in parallel for each experiment and used …
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Triggerable ligand presentation using caged-RGD
Cells rely on time-dependent binding and activation by the ECM to initiate downstream signal transduction. It is unknown whether adhesion to a ligand is required throughout various cell processes, or only during a specified time period ("temporal threshold”). Current approaches to ligand …
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Small Molecules as Probes for Cell Division and Intracellular Transport
<p>Cell-permeable small molecules that can act on their targets on fast time scales are powerful probes of cell division mechanisms and intracellular transport processes. Phenotype-based screens with chemical libraries have been used to identify such inhibitors. However, probes for most proteins …
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The role of the chromatin remodeller ISWI in the cellular response to hypoxia
… Factor (HIF) is a master regulator of the cellular response to hypoxia. Its levels and activity are controlled by dioxygenases called Prolyl-Hydroxylases and Factor Inhibiting HIF (FIH). In order to activate genes, HIF has to access sequences in DNA that are integrated in chromatin. It is …
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Cellular clocks on steroids: the mechanism of circadian entrainment by glucocorticoids
… by which GCs synchronize and reset the phase of cellular clocks is not completely understood. A plausible model has been proposed, following the classical genomic signalling pathway of its cognate nuclear hormone receptor, the glucocorticoid receptor (GR). The GR activates the transcription of …
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Functional analyses of microtubule and centrosome-associated proteins in Dictyostelium discoideum
… at the centrosome throughout the entire cell cycle. The protein was also detected at microtubule plus ends, however, unexpectedly only during interphase but not during mitosis. The same cell cycle-dependent localization pattern was observed for CP224, the Dictyostelium XMAP215 homologue. …
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Dynamics of DNA-repair factors and chromosomes studied by laser-UVA-microirradiation and laser-photobleaching
… to follow dynamical nuclear processes during the cell cycle and during DNA-repair. Laser-UVA-microirradiation The protein Rad51 is essential for the repair of double-strand breaks (DSBs) via the conservative homologous recombination repair pathway. To test the hypothesis that Rad51 localizes to …
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Role of Microtubule Motor Proteins in Adenoviral Infections
… to protect the viral genome outside of host cells. However, they are able to orchestrate a precise disassembly program initiated once the next susceptible cell is reached, leading to step-wise virus entry, controlled capsid disintegration, efficient DNA delivery, and production of progeny …
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Endoplasmic Reticulum Morphoregulation Modulates Ca2+- Driven Brain Cell Functions
… network of tubules that extend throughout the cell. One of the essential functions of the ER is to store large amounts of calcium (Ca2+ ) and sequester the ion on low-affinity/high-capacity protein carriers. This enables the ER to rapidly release Ca2+ in response to specific signals, either …
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Cytotoxic T lymphocyte biology under the microscope
… out and destroy virally infected or cancerous cells. Incapacitate them and you become severely immunocompromised and riddled with tumours (à la prf1-/- mouse model); enhance them and you can beat previously unbeatable cancers (e.g., CAR T cell therapy). More than this though, they are …
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Antimalarial benzimidazoles and related structures incorporating an intramolecular hydrogen bonding motif: medicinal chemistry and mechanistic studies
… in vitro against the Chinese Hamster Ovary cells. The N-benzyl benzimidazole analogues, designed based on the known antihistamine drug astemizole, were tested against the hERG (human ether-a-go-go-related)-encoded potassium ion channel. These analogues expressed >40% inhibition against the …
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Protein folding in living cells and under pressure
… is impacted by immersion in the crowded intracellular environment and explore whether perturbations to the intracellular folding landscape can be linked to protein function. A review of the forces at play in the intracellular environment and the role that ultra-weak “quinary” interactions play …