Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 125 for “"live-cell imaging"”.
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Fluorogenic probes for live-cell imaging of biomolecules
… application of these probes to study dynamic cell transport processes. Chapter 1. Enzyme-Activated Fluorogenic Probes for Live-Cell and In Vivo Imaging. Chapter 1 reviews recent advances in enzyme-activated fluorogenic probes for biological imaging, organized by enzyme classification. This …
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DEVLOPEMNT OF FLUORESCENT IMAGING TOOLS FOR LIVE CELL IMAGING
… there is a growing interest to understand cellular processes and behaviors in real time. Fluorescent live cell imaging presents an appealing approach to learn about cell dynamics and function by providing specificity and high resolution. However, one of the biggest limitations of these …
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Trimethoprim-Based Chemical Tags for High Resolution Live Cell Imaging
… to complement the use of fluorescent proteins in live cell imaging. Chemical tags retain the specificity of protein labeling achieved with fluorescent proteins through genetic encoding, but provide smaller, more robust tags and modular use of organic fluorophores with high photon-output and …
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Live cell imaging of protein trafficking: Dynamics and functional insights
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-08-01
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LIVE CELL IMAGING BY INTEGRATING FLUORESCENCE AND MOTION- ENHANCED DIFFERENTIAL INTERFERENCE MICROSCOPY
… Now, we have developed methods for simultaneous imaging using short wavelength DIC/MEDIC and standard fluorescence microscopy. Our novel method of integrating MEDIC and fluorescence imaging provides the tag-free contrast-enhanced advantages of MEDIC and the high specificity of fluorescence …
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New tools for live cell imaging of endogenous loci in mammalian cells
Embargo set by: Seth Robbins for item 116313 Lift date: 2022-10-07T22:50:13Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system
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Rational design and directed evolution of probe ligases for site-specific protein labeling and live-cell imaging
… However, in order to use these probes for live-cell protein imaging, highly specific labeling methods are required. Here, we will describe three efforts to re-engineer the E. coli enzyme, lipoic acid ligase (LplA), to catalyze the ligation of small-molecule probes onto recombinant proteins. …
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Compressed fluorescence lifetime imaging microscope
Fluorescence lifetime imaging microscopy (FLIM) has been extensively applied in wide biomedical applications from single cell studies to medical diagnosis. While the state-of-the-art FLIM techniques are highly sensitive to biological dynamics such as fluorescence resonance emission transfer (FRET), …
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Chemical Tools for Exploring IFITM3 S-Palmitoylation and Mechanism
… of S-palmitoylated proteins in dendritic cells and macrophages by our laboratory revealed that the interferon-induced transmembrane proteins (IFITMs) are S-fatty-acylated at conserved cysteine (Cys) residues. IFITMs are unique interferon-induced proteins that restrict the infection of …
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Development of a device for multi-modal mechanical manipulation of cells in 2D and 3D engineering environment
All organisms from bacteria to cells within the human body experience some form of mechanical stimuli. The biochemical response from mechanical stimuli is known as mechanotransduction. Cell manipulation devices provide an understanding of mechanotransduction and the various signalling mechanisms …
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Development of a tool for tracking H3K27 tri-methylation
… inducible fluorescent modification-specific intracellular antibody (mintbody) that reversibly binds to post-translational modifications to tag the H3K27 trimethylation. Four cell lines were created using a mintbody including super folded GFP that can be induced with treatment with doxycycline. …
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Microfluidics and live imaging advances: applications in host/pathogen, immunity and stem cell single cell phenotyping
Live single-cell imaging has emerged as an advanced single-cell study tool for approaching a quantitative understanding of many biological questions in recent years. In previous cell studies using bulk cell measurements, the population averages can miss the information from cell to cell variability …
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Design and implementation of the SBX1: a smart environment chamber for biological research and discovery
… chambers for incubation and examination of live cell samples. They require precise control over temperature, humidity, and gas concentration to mimic natural conditions for cell survival and growth. Many incubators and live cell imaging systems exist as commercial products; however, they are …
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Pil1, an Eisosome Organizer, Plays an Important Role in the Recruitment of Synaptojanins and Amphiphysins to Facilitate Receptor-Mediated Endocytosis in Yeast
… role in receptor-mediated endocytosis. Using live cell imaging, I show that endocytic patches carrying Abp1 and Las17 persisted much longer in PIL1-deficient cells (pil1Δ). The loss of Pil1 also greatly affected both the scission efficiency and the frequency of formation of endocytic sites …
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Migration of Nucleocapsids in Vesicular Stomatitis Virus-Infected Cells is Dependent on Microtubules and Actin Filaments
… and organelles owe their distribution within cells to their affinities to the cytoskeleton and cellular membranes. This cargo is carried along the cytoskeleton and membranes using a variety of cytoskeletal motors and adapter proteins. Viruses hijack these pathways to distribute their proteins …
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An Optical Flow Based Methodology for Visualizing Dynamic Sucellular [sic] Organiztion [sic] Demonstrated Through Profilin and Rho GTPase Microdomains
Live cell imaging has enabled the collection of movies of subcellular protein dynamics at a submicron resolution. Statistical time series analysis can greatly expand our understanding of subcellular interactions in minimally perturbed systems. This was previously achieved for the leading edge of …
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DNA Origami Structures for Artificial Light-Harvesting and Optical Voltage Sensing
… be used as a sensitive voltage sensor for live-cell imaging of transmembrane potentials.
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Dynamics of Cell Fate Decisions Mediated by the Interplay of Autophagy and Apoptosis in Cancer Cells: Mathematical Modeling and Experimental Observations
… biological stress response in mammalian cells that is responsible for clearing damaged proteins and organelles from the cytoplasm and recycling their contents via the lysosomal pathway. In cases where the stress is not too severe, autophagy acts as a survival mechanism. In cases of severe …
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Mechanisms of dendritic peptide release
Magnocellular neurones (MCNs) are capable of secreting vasopressin and oxytocin from the somato-dendritic compartment, which can occur independently to secretion from nerve terminals. One hypothesis of the mechanism that regulates this differential release is that dendrites utilise different …
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