Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 94 for “"live imaging"”.
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Interpreting Notch signals: characterisation of dynamic transcription hubs by live imaging
… translates in the activation of target genes. A live imaging approach was taken to understand the mechanisms for robust activation of Notch targets, focusing on the co-activator Mastermind, a key component of the Notch transcription complex. Specifically, its enrichment and behaviour were …
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Static and Microfluidic Live Imaging Studies of Plasmodium falciparum Invasion Phenotypes
… firstly involved the development of an optimised imaging platform for recording egress-invasion sequences. I used live cell microscopy to understand this stage of malarial infection better, by monitoring egress-invasion sequences in live cultures under controlled conditions and addressing the …
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Optimising light-sheet microscopy for high resolution live imaging of the early development of chick embryos
Live imaging plays a key role in the study of biological processes ranging from the sub-cellular to organism scale, and all the scales between. This requires the development and use of ever evolving innovative microscopies.<br/><br/>The thesis project presented in the following pages describes the …
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Microfluidics and live imaging advances: applications in host/pathogen, immunity and stem cell single cell phenotyping
Live single-cell imaging has emerged as an advanced single-cell study tool for approaching a quantitative understanding of many biological questions in recent years. In previous cell studies using bulk cell measurements, the population averages can miss the information from cell to cell variability …
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The Development of a Printable Device with Gravity-Driven Flow for Live Imaging Glioma Stem Cell Motility
… fabricating PDMS microfluidics. This printable imaging device allows for higher throughput live-imaging of cell lines to aid in the understanding of the relationships between intratumoral heterogeneity, invasion dynamics, and interstitial flow.
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Analysis of embryonic development in Tribolium castaneum using a versatile live fluorescent labelling technique
… advanced tools for fluorescent labelling of live embryos. In my work, I have used a transient method for strong, homogeneous and persistent expression of fluorescent markers in Tribolium embryos, labelling the chromatin, membrane, cytoskeleton or combinations thereof. I have used several of …
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Mechanisms underlying rapid activity-dependent structural plasticity at Drosophila neuromuscular junctions
… in response to elevated neuronal activity. Using live imaging of synaptic growth, we characterized this dynamic process and demonstrate that rapid bouton budding requires retrograde BMP signaling and local alteration in the presynaptic actin cytoskeleton. BMP acts during development to provide …
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Investigating processing body condensation, material properties and function during early Drosophila development
… Using pharmacological disruption and live imaging, I show that large scale cytoplasmic reorganisation causes P body dispersal during the oocyte to embryo transition to release the stored maternal mRNAs. Finally, in the early embryo, using live imaging and biochemical analyses, I show …
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In vivo imaging and morphogenesis of butterfly scale development
… studying structure formation in wing scales of live butterflies with high temporal and spatial resolution. To gain a comprehensive perspective of scale formation, I developed pupal surgery techniques and adapted quantitative phase microscopy methods to achieve label-free, in vivo visualization …
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A Study of Mouse Peri-Implantation Morphogenesis
… embryos and understand its mechanism through live imaging and inhibitor treatments (1). Then, I aimed to examine how the interplay between the polar trophectoderm and epiblast shapes the epiblast tissue comparing mouse and human embryos upon implantation (2). Following the study of epiblast …
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Advanced in vivo imaging of the interactions between vascular and renal development in the zebrafish pronephric kidney
… zebrafish provides a research model for advanced imaging technologies, allowing us to visualise structures located deep within living specimens. Here, we combine deep-tissue live imaging and novel functional assays to study development and function of the pronephric kidney - the first and most …
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GENERATION AND ANALYSES OF krasV12 TRANSGENIC ZEBRAFISH TO STUDY INTESTINE TUMORIGENESIS
… specific promoter and the EGFPkrasV12 enabled live imaging. The expression of oncogenic krasV12 caused highly proliferative epithelial outgrowths in to the lumen. Long term induction of krasV12 resulted in tubular adenoma of the adult fish along with significant increase in p-Erk and …
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Imaging Neuromuscular Synaptogenesis
… Here, using whole-mount high-resolution live imaging, we show that glutamatergic synaptogenesis on all muscles in Drosophila embryos is preceded by formation of a myopodial cluster into which PSD-95/Dlg is rapidly recruited. Cell-specific disruption of myopodial clustering affect …
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Deciphering the biology of axon stretch-growth
… two approaches. First, a device was created for live imaging of stretch-growth as it occurs on the stage of a microscope. Morphology changes and cytoskeletal transport were captured live at up to 600x magnification over six days of culturing. Second, the RNA species produced during stretch-growth …
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MECHANOPROPERTIES, HETEROGENEITY AND CELL MIGRATION IN GLIOBLASTOMA
… SP2G (SPheroid SPreading on Grids), the live imaging of GBM spheroids spreading on grid micropatterns mimicking the brain vasculature. To counteract the issues in PDX and rapidly identify the most invasive sub-populations hidden in heterogeneous GBMs, we developed an in vivo mimicry …
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Developing an a-synuclein in vitro model for therapeutic testing for Parkinson's disease
… cortical neural cells. This involves a 14-day live imaging study of α-synuclein pre-formed fibril (PFF) seeding, α-synuclein PFF cellular uptake, induced pathology, and characterisation of the model, including morphological and phenotypic changes, as well as investigations into synapse loss and …
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Investigating the Role of Insulin-Like Signaling in Paclitaxel-Induced Nociceptive Hypersensitivity using Drosophila melanogaster
… did not completely resolve at the larval stage. Live imaging of peripheral thermal nociceptors showed that this hypersensitivity precedes observable neuronal damage. At low concentrations, PTX caused hyper-sprouting of tertiary dendritic spines in peripheral nociceptors. At high concentrations, …
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Epidermis and Re-epithelialization in <i>Schmidtea mediterranea</i>
… in detail in planarians. Our work introduces live imaging methodology, which allows following epidermal cells and their response to tissue damage or tissue loss for extended time (hours) and in high resolution. Using our methods, we followed planarian cells live for the first time and in …
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Damage-Induced Inflammation and Nociceptive Hypersensitivity In Drosophila Larvae
… cells and blood cells (hemocytes). Using live imaging, we monitored the circulatory dynamics of hemocytes and the methods by which they accumulate at epidermal wounds. We found that circulating hemocytes attach to wound sites directly from circulation, a mechanism once thought to work …
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Dividing the Preplate: Characterization of Neuronal Subpopulations in the Early Murine Cerebral Cortex
… I applied birthdating analyses and live imaging to describe the proliferative and migratory characteristics of subpopulations of Cajal-Retzius and subplate neurons. Purified subpopulations were used in a gene expression array analysis to define mRNAs differentially expressed between …
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