Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 156 for “"live cells"”.
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Studying Processive Molecular Motors Inside Live Cells
… motor proteins in vivo, in other words, inside live cells. In order to overcome the complications of imaging in live organisms we have tracked the cargos (organelles carried by motors) instead of single molecule markers bound to the motors. Fluorescently labeled peroxisomes and dark pigment …
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Protein folding and diffusion: from in vitro to live cells
… to modulation by many factors inside living cells: crowding, electrostatics, hydrophobic interactions, and even hydrodynamic phenomena. The resulting spatio-temporal fluctuations in protein folding rates, protein stability, protein diffusion, as well as protein structure, protein function, …
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Single-molecule G-quadruplex visualisation in live cells and 3D chromatin
… visualisation of G4 structures in living cells. Single-molecule imaging was performed at nanomolar probe concentrations providing measurements of relative populations of G4s in living cells without global perturbation of G4 dynamics. Chemical trapping of the unfolded G4 state reveals that …
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Spatially-resolved transcriptomic mapping in live cells using peroxidase-mediated proximity biotinylation
The spatial organization of RNA within cells is crucial for the regulation of a wide range of biological functions, spanning all kingdoms of life. However, a general understanding of RNA localization has been hindered by a lack of simple, high-throughput methods for mapping the transcriptomes of …
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New reporters of protein trafficking and protein-protein interactions in live cells
… localization and protein-protein interactions in live cells. We first attempted to detect endogenous protein-protein interactions (PPIs) in live cells by means of a ribozyme complementation assay, but we found that the strategy was limited by the interaction affinity constraints and by low …
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Using Advanced Fluorescence Microscopy to Analyze Intracellular Trafficking of FcRn In Live Cells
The file named "GanZhou.pdf" is the primary dissertation file. All other files ("*.mov") are supplemental files and may be viewed individually.
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Revealing the transposable element dynamics driving evolutionary transitions through direct observation in live cells
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2024-12-01
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Determining S6K1 localisation and interactions with mTORC1 in live cells using fluorescence lifetime imaging microscopy
… S6K1 occurring mainly in the cytoplasm of living cells (from τm=2.5 to 2.3 ns). In Chapter 4, AZD2014 and INK128, both pan-mTOR inhibitors, show fluorescent properties that can be used to investigate their cellular action. The fluorescence quantum yields for AZD2014 and INK128 are 0.47 and 0.33, …
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Intracellular labeling of live cells via transient membrane permeabilization for fluorescence and super resolution microscopy
… for labeling intracellular proteins of living cells. The technique makes use of Streptolysin O (SLO), a bacterial enzyme that permeabilizes cells to DNA, RNA, proteins. We show that SLO can be used to deliver a variety of fluorescent probes; ranging from organic dyes (<1 kDa in size) to …
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Rapid Rac GTP-ASE activation in live cells by mechanical stress is independent of Src
… stresses to living human airway smooth muscle cells with a magnetic bead bound to the cell surface via transmembrane adhesion molecule integrins. The temporal and spatial activation of Rac, a small guanosine triphosphatase, was quantified using a fluorescent resonance energy transfer (FRET) …
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Delivery of molecular-specific optical contrast agents for cancer biomarker detection in live cells and tissues
… been hindered, however, by the difficulty of delivering macromolecules through mucosal tissue. My goal was to develop a robust strategy for the delivery of molecular-specific optical contrast agents into live cells and tissues. Specifically, I sought to: (1) develop an efficient and reproducible …
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Effects of micro- and nano-scale surface geometry on behaviors of live cells and liquid droplets
… surface geometry also affects biological cells by altering their mechanical environment. In the case of cell mechanics we generally lack even a flawed model for describing the observed behavior, so we seek to identify parameters that might simplify these cellular systems. One method that …
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Synthesis and characterization of new light emitting probes for sensitive detection of bio-molecules and live cells
… gating of the signal, which avoids the short-lived background of interfering sample components. This property in combination with large Stokes shift contributes to extreme sensitivity of detection (ca. 10^{-13}-10^{-14} M), which makes lanthanide-based probes suitable for large variety of …
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Interaction Forces And Reaction Kinetics Of Ligand-Cell Receptor Systems Using Atomic Force Microscopy
… samples from proteins, DNA to living cells. We developed AFM measurement procedures to measure protein interactions on live cells at the single molecular level. These measurements can be interpreted by using proper statistical approaches and can yield important parameters about …
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Development of an in vitro model of contraction by fibroblasts
… contraction. In addition, observation of live cells contracting individual matrix struts established the cellular mechanisms responsible for the matrix contraction measured in the CFM. Observation of live cells revealed that macroscopic contraction of the collagen-GAG matrix was the result …
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Optimization of fluorescence lifetime imaging microscopy (FLIM) for studying the activity of enzymes in live cancer cells
… in order to observe the dynamics of enzymes in live cancer cells. The enzyme studied throughout this research is Membrane Type 1 Matrix Metalloproteinase (MT1-MMP) which is a membrane-bound protein principally responsible for degrading extra-cellular matrix (ECM) proteins in the local …
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Dynamics of Genome Organization
… First, by pulling on a genomic locus in live cells, we revealed the rather liquid-like material properties of chromatin and dispelled the idea that chromatin in interphase forms a gel. Second, by tracking genomic elements known to act as boundary elements for loop formation, we quantified …
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Development and application of correlative STED and AFM to investigate neuronal cells
… was combined with a commercial AFM to study live cells. Using the recently developed SiR-actin and SiR-tubulin dyes and AFM probes optimised for live cell mechanical property studies, images of the actin and tubulin cytoskeleton were correlated with AFM topography and mechanical stiffness …
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Functional studies on receptor-type protein tyrosine phosphatases of the R3 subgroup
… fluorescence complementation (BiFC) assay in live cells to examine the molecular basis for the interaction of one of the R3 RPTP members, VE-PTP, with VE-cadherin, and explored the potential of others to interact with this protein. The potential of R3 RPTPs to homo-dimerise via extracellular …
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