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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.

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Showing 1 to 20 of 233 for “"live cell"”.

  1. Optical rheology for live cell membranes

    Park, YongKeun, S.M. Massachusetts Institute of TechnologyMassachusetts Institute of Technology2007

    … retrieving the full viscoelastic information of cell membrane material properties. Red blood cells (RBC) are chosen for this study because of their simple structure, which consists of a bi-layer cell membrane supported by a cytoskeleton enclosing a homogeneous fluid. The full complex modulus of …

    mit Repository record

  2. Optogenetic regulation of protein activity in live cell

    Mondal, PayelUniversity of Illinois at Urbana-Champaign2020

    … among each other and result in different cellular phenotypes depending on the dynamic profile of protein activity. Conventional genetic and pharmacological approaches such as gene overexpression, use of growth factors or inhibitors have helped us delineate interaction maps of signaling …

    uiuc Repository record

  3. Fluorogenic probes for live-cell imaging of biomolecules

    Chyan, Wen, Ph. D. Massachusetts Institute of TechnologyMassachusetts Institute of Technology2018

    … application of these probes to study dynamic cell transport processes. Chapter 1. Enzyme-Activated Fluorogenic Probes for Live-Cell and In Vivo Imaging. Chapter 1 reviews recent advances in enzyme-activated fluorogenic probes for biological imaging, organized by enzyme classification. This …

    mit Repository record

  4. DEVLOPEMNT OF FLUORESCENT IMAGING TOOLS FOR LIVE CELL IMAGING

    SAPOZNIK, ETAI SAPOZNIKWake Forest University2017

    … there is a growing interest to understand cellular processes and behaviors in real time. Fluorescent live cell imaging presents an appealing approach to learn about cell dynamics and function by providing specificity and high resolution. However, one of the biggest limitations of these …

    wfu Repository record

  5. New optical techniques and hardware for studying live cell dynamics

    Tinning, Peter WilliamUniversity of Strathclyde2018

    … and has opened the door to investigating live biological specimens non-invasively at sub-cellular spatial resolutions with high specificity and temporal resolutions. One of the limiting factors of optical microscopy is that the spatial resolution is dictated by the diffraction limit of …

    strathclyde Repository record

  6. Trimethoprim-Based Chemical Tags for High Resolution Live Cell Imaging

    Jing, Chaoran2013

    … to complement the use of fluorescent proteins in live cell imaging. Chemical tags retain the specificity of protein labeling achieved with fluorescent proteins through genetic encoding, but provide smaller, more robust tags and modular use of organic fluorophores with high photon-output and …

    columbia-diss Repository record

  7. Photonic crystal enhanced microscopy for imaging of live cell adhesion

    Chen, WeiliUniversity of Illinois at Urbana-Champaign2014

    … (EF) imaging modalities for the study of cell-surface interactions and nanoparticle-based assays. The imaging system utilizes an angle-tunable illumination source, either LED or laser, to achieve highly efficient coupling of incident light to PC optical resonances. Detection of the …

    uiuc Repository record

  8. Live cell imaging of protein trafficking: Dynamics and functional insights

    Mehta, KritikaUniversity of Illinois at Urbana-Champaign2024

    Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-08-01

    uiuc Repository record

  9. LIVE CELL IMAGING BY INTEGRATING FLUORESCENCE AND MOTION- ENHANCED DIFFERENTIAL INTERFERENCE MICROSCOPY

    Ray, Steven LaneWake Forest University2012

    Optical microscopy's resolution is limited by the wavelength of illuminating light and can be improved by selecting shorter wavelengths. The contrast of optical microscopy, which along with resolution, ultimately determines image quality and is enhanced in techniques such as differential …

    wfu Repository record

  10. New tools for live cell imaging of endogenous loci in mammalian cells

    Tasan, IpekUniversity of Illinois at Urbana-Champaign2020

    Embargo set by: Seth Robbins for item 116313 Lift date: 2022-10-07T22:50:13Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system

    uiuc Repository record

  11. Live cell proximity biotinylation enables proteomic analysis of mitochondria-endoplasmic reticulum contact sites

    Hung, VictoriaMassachusetts Institute of Technology2016

    The cell is able to carry out an astounding number of biological functions in part due to its ability to compartmentalize chemical reactions to different cellular regions and organelles. In order to understand the biological pathways that occur in a specific cellular region, one needs to know the …

    mit Repository record

  12. Live-cell imaging of multiple endogenous mRNAs permits the direct observation of RNA granule dynamics

    Yatsuzuka, KenjiKyoto University2019

    kyoto Repository record

  13. Rational design and directed evolution of probe ligases for site-specific protein labeling and live-cell imaging

    White, Katharine AliceMassachusetts Institute of Technology2012

    … However, in order to use these probes for live-cell protein imaging, highly specific labeling methods are required. Here, we will describe three efforts to re-engineer the E. coli enzyme, lipoic acid ligase (LplA), to catalyze the ligation of small-molecule probes onto recombinant proteins. …

    mit Repository record

  14. Optimization Techniques for Multi-object Detection and Tracking on Live-cell Fluorescence Microscopy Images and Their Applications

    Wang, MengfanVirginia Tech2024

    … processes and has been extensively utilized in live-cell morphology analysis. Despite its utility, related object detection and tracking tasks still face challenges due to large data scales, inferior data quality, and insufficient annotations, leading to reliance on adaptive thresholding. …

    vt Repository record

  15. Single-molecule live cell tracking and super-resolution microscopy to study stress related oligomerization and aggregation of the ALS-related protein TDP-43

    Streit, LisaUniversität Ulm

    ulm-diss Repository record

  16. GFP- coated microparticles to quantify and compare wild- type desmin with known desmin mutations in human heart disease via quantitative live-cell fluorescence Imaging

    Al-Aridhi, TaniaUniversität Bielefeld

    … of many biological processes in living cells. Revealing the full potency of modern microscopy in biology systems requires extracting quantitative information from fluorescence microscopy. There is a persistent need for an unbiased method for reproducible processing and quantifying by …

    bielefeld Repository record

  17. Design and implementation of the SBX1: a smart environment chamber for biological research and discovery

    Ball, Daniel S., author, Chen, Thomas, advisor, Wilson, Jesse, committee member, Chicco, Adam, committee memberColorado State University. Libraries2021

    … chambers for incubation and examination of live cell samples. They require precise control over temperature, humidity, and gas concentration to mimic natural conditions for cell survival and growth. Many incubators and live cell imaging systems exist as commercial products; however, they are …

    colostate Repository record

  18. Entwicklung eines SMI-Mikroskops zur Präzisions-Strukturanalyse in vitalen biologischen Systemen

    Reymann, JürgenUniversität Bielefeld

    … adapting this technique to the study of live cells and testing its applicability for such in-vivo procedures. By using spatially modulated illumination, SMI microscopy achieves an increase of the information content for object structures with dimensions below the conventional resolution. …

    bielefeld Repository record

  19. Compressed fluorescence lifetime imaging microscope

    Ma, YayaoUniversity of Illinois at Urbana-Champaign2019

    … in wide biomedical applications from single cell studies to medical diagnosis. While the state-of-the-art FLIM techniques are highly sensitive to biological dynamics such as fluorescence resonance emission transfer (FRET), they depend on repetitive measurements and the slow frame rate limits …

    uiuc Repository record

  20. Molecular Architecture of the ER-Mitochondria Encounter Structure

    Wozny, MichaelUniversity of Cambridge2022

    … (MCS) form between all organelles of eukaryotic cells and facilitate interdependent processes between unique subcellular compartments. In budding yeast, MCS between the endoplasmic reticulum (ER) and mitochondria are mediated by a protein complex known as the ER-mitochondria encounter structure …

    cambridge Repository record

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