Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 61 for “"liquid-liquid phase separation"”.
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Quantitative analysis of protein liquid-liquid phase separation
… proteins tend to demix into a protein-rich liquid condensate phase and a protein-poor dilution phase. This process of liquid–liquid phase separation (LLPS) has emerged as a crucial mechanism for describing the production of biological condensates in live cells, which have been found to be …
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Asssessing the Liquid-Liquid Phase Separation Abilities of Eyes Absent Protein
… EYA TADs were identified as potential drivers of liquid-liquid phase separation in a proteome-wide screen. The apparent conservation of this physical potential within the disordered region of EYA proteins suggests that phase separation of this region may play a role in interactions between EYA and …
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Light scattering by aqueous protein solutions that exhibit liquid-liquid phase separation
Thesis (Ph. D.)--Massachusetts Institute of Technology, Whitaker College of Health Sciences and Technology, 1994.
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The Nucleolar Protein Nucleophosmin Undergoes Liquid-Liquid Phase Separation with Arginine-Rich Nucleolar Proteins through Weak, Multivalent Electrostatic Interactions
… (LCDs) and rRNA have been shown to promote liquid-liquid phase separation (LLPS) in vitro, suggesting a basis for the liquid-like behavior of nucleoli. Nucleophosmin (NPM1), a multifunctional and highly abundant nucleolar protein, exhibits structural features associated with LLPS suggesting …
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MOLECULAR MECHANISMS OF ATP-MODULATED LIQUID-LIQUID PHASE SEPARATION (LLPS) OF TDP-43 AND SARS-COV-2 NUCLEOCAPSID PROTEIN
An estimated 40% of human proteome has undergone LLPS; delineating its molecular mechanisms may reveal principles undiscovered in physiology and open avenues for pathology. All living cells inexplicably maintain 2-14 mM ATP. Strikingly, in 2017, ATP (> 5 mM) was demonstrated to behave as a …
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The role of DAXX in the selective autophagy receptor SQSTM1/p62 phase condensation
… various biological processes, are assembled via liquid-liquid phase separation (LLPS). These phase-separated condensates ensure that cellular activities occur in a spatiotemporally controlled manner and provide a method for concentrating and segregating cellular components. Liquid-like …
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Investigation of physical principles of protein phase transitions
Protein phase transitions, including liquid-to-solid aggregation and liquid-liquid phase separation, are fundamental processes underlying neurodegenerative disorders such as Alzheimer’s (the Aβ peptide and the tau protein), Parkinson’s (the α-synuclein protein), and Huntington’s diseases (the Htt …
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Crystallization Kinetics and Phase Behavior of Molecular Solutions
… selectivity; and (ii) to develop a generalized phase diagram from first principles and verify its applicability to a wide range of molecular solutions. This thesis investigates how the rate of supersaturation affects macroscopic phase transitions and final crystal properties and how the …
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On the physics of intranuclear organization
… feat by leveraging physical processes such as liquid-liquid phase separation driven by multivalent macromolecular interactions to form biomolecular condensates which can serve as membrane-less organelles for the precise, vectorial organization of intranuclear contents. In particular, the …
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Formation of optimised particles for formulation and processing
… under specific process conditions involving liquid-liquid phase separation; hence ternary phase diagrams were determined experimentally. The liquid-liquid phase region was found to increase with increasing temperature, whilst absent at 20°C. Ibuprofen agglomerates crystallised in the presence …
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RNA-Peptide Based Coacervates as a Prebiotically Plausible Pathway towards a Proto-Ribosome
… a new pathway emerges. Coacervation, a liquid-liquid phase separation event where two or more molecules undergo non-covalent interactions to form a secondary liquid phase, has been studied as a method of prebiotic compartmentalization. This thesis uses coacervates to mimic the peptide …
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Self-organization of Microtubule and Associated Protein Map65
… results of the MAP65 condensate formation via liquid-liquid phase separation and quantification of their properties. Additionally, I elucidate a mechanism of non-centrosomal microtubule nucleation that may play an essential role in the mitotic spindle.</p>
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Absorption based processes applying phase transition for energy efficient postcombustion CO2 capture
… is energy intensive and thus costly. The novel phase transitional CO2 absorption process has been regarded as a promising alternative to the benchmark MEA process. The solvent in a phase transitional process turns into two phases after CO2 absorption; one phase contains the majority of the …
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Transcriptional regulation in Plasmodium falciparum: characterisation of TBP-like protein
… has been mounting evidence that IDRs mediate liquid-liquid phase separation, a process which has transformed our understanding of cellular organisation and regulation of key cellular processes. The functional relevance of these IDRs is unknown. Therefore, to elucidate their role, this study …
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Characterizing Multivalent Interactions Between Folded Protein Domains and Intrinsically Disordered Regions or Peptide Substrates
… the interdomain interactions that mediate the liquid-liquid phase separation (LLPS) of ubiquilin-2 (UBQLN2). UBQLN2 is a shuttle protein for the proteasome that functions by binding to proteasomal receptors and ubiquitinated substrates via its N-terminal ubiquitin-like (UBL) and C-terminal …
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Investigating processing body condensation, material properties and function during early Drosophila development
… ribonucleoprotein assemblies that form via liquid-liquid phase separation. Conserved across eukaryotes, Processing bodies (P bodies) are cytoplasmic biomolecular condensates which act as hubs for RNA regulation including storage, translation, and degradation. During Drosophila oogenesis, …
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β2-mikroglobulino gryninimas ir skysčio-skysčio fazių atsiskyrimo tyrimai /
… A rapidly growing area of research is liquid-liquid phase separation (LLPS), the process by which proteins and other biomolecules separate from the bulk solution to form dense, membraneless condensates. Although dozens of proteins have been identified to form condensates, there is …
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Dissolution Behavior of Amorphous Solid Dispersions
… suggests that some systems can undergo another phase transformation prior to crystallization, namely liquid-liquid phase separation (LLPS). LLPS creates a discrete drug-rich phase dispersed in the continuous aqueous drug-lean phase and is expected to impact the bioavailability of the drug though …
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Investigating the dynamic nature of biomolecular condensates that support rotavirus replication
… The formation of such factories is driven by liquid-liquid phase separation (LLPS) of an intrinsically disordered phosphoprotein NSP5, which acts as a scaffold, along with the RNA chaperone NSP2 as the major client biomolecule. While viroplasms have been recognised as ribonucleoprotein …
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Liquid-Liquid Phase Separations in Innate Immune DNA Sensing and NF-κB Signaling Pathways
… to cGAS robustly induced the formation of liquidlike droplets in which cGAS was activated. The disordered and positively charged cGAS N terminus enhanced cGAS-DNA phase separation by increasing the valencies of DNA binding. Long DNA was more efficient in promoting cGAS liquid phase …
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