Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 76 for “"liquid chromatography-mass spectrometry (LC-MS)"”.
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Targeted and untargeted metabolomic profiling of human serum, urine and brain by liquid chromatography mass spectrometry (LC-MS)
… develop, metabolomic profiling methods based on LC-HRMS for application to different human biofluid and tissue samples.;In the first study concerns the potential of metabolic changes to give an indication of physical fitness. Urine samples were collected from ten physically active subjects who …
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Continuous flow fast atom bombardment and electrospray ionization mass spectrometry in natural products research
… atom bombardment (CF-FAB) has been applied as a liquid chromatography/mass spectrometry (LC/MS) and liquid chromatography/mass spectrometry/mass spectrometry (LC/MS/MS) interface in natural products research. Initial studies concentrated on characterization of the interface, optimization of …
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Identification and Quantification of Protein Carbonylation by Mass Spectrometry
… protein in this system. The research is based on liquid chromatography/mass spectrometry (LC/MS), Western Blots, and enzymatic assay.
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Using non-targeted mass spectrometry to identify proteomic, metabolomic and lipidomic profiles in saliva to compare the impact of prolonged vs. interrupted sitting
… a non-targeted approach with the use of Liquid Chromatography-Mass Spectrometry (LC-MS) to assess the responsiveness of proteins and metabolites in saliva to sedentarism was employed. The study involved 24 participants that engaged in both a prolonged sitting session and an interrupted …
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Multiplatform metabolome profiling to identify specific signatures and biomarkers in blood samples: untargeted approach
… techniques such as vibrational spectroscopy and liquid chromatography-mass spectrometry (LC-MS) was evaluated in different studies related to the field of health and disease. Thus, this doctoral thesis's main objective is to provide an objective diagnosis of disorders such as Parkinson’s, …
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Comprehensive proteomic profiling of clinically relevant strains of Mycobacterium tuberculosis
… discrepancies in phenotypic presentation in terms of virulence, elicited immune response and transmissibility. This study aims to use Mass Spectrometry (MS) tools to quantitatively and qualitatively investigate the total proteome expressed by various epidemiologically significant strains within …
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Application and Characterization of Bioactive Compounds in Peanut Skins, a Waste Product of Virginia Agriculture
… of a silica or size exclusion high performance liquid chromatography (HPLC) column. Fractions were then subjected to a yeast growth curve assay to determine the active fractions. The fractions were then characterized by liquid chromatography-mass spectrometry (LC-MS). Negative mode electrospray …
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Extraction and analysis of pharmaceutical residues in environmental samples using SPE with LC-MS/MS
… based upon solid phase extraction (SPE) and liquid chromatography mass spectrometry (LC-MS). Monolithic silica based stationary phases were used for the development of high performance liquid phase separations of common pharmaceuticals, the antifouling and anti-dandruff agent zinc pyrithione …
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Liquid Chromatography – Mass Spectrometry for Protein Biomarker Discovery in Pancreatic Adenocarcinoma
… in its early stages. Recent developments in liquid chromatography – mass spectrometry (LC-MS) technology have made it possible to profile proteins associated with disease in a comprehensive manner. Within this thesis current LC-MS methodology was applied to the problem of pancreatic cancer. A …
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Metabolomic approaches to understanding the auxin and ethylene response in Arabidopsis roots
Non-targeted metabolite profiling by liquid chromatography-mass spectrometry (LC-MS) was used to determine the metabolite responses of Arabidopsis roots to auxin or ethylene. Crosstalk between these hormones regulates many important physiological processes in plants, including the initiation of …
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Computational Analysis of LC-MS/MS Data for Metabolite Identification
Metabolomics aims at the detection and quantitation of metabolites within a biological system. As the most direct representation of phenotypic changes, metabolomics is an important component in system biology research. Recent development on high-resolution, high-accuracy mass spectrometers enables …
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Changes in the Rpb3 Interactome Caused by the Deletion of RPB9 in Saccharomyces cerevisiae
… identification technology (MudPIT), a form of liquid chromatography-mass spectrometry (LC-MS). This data was searched using the SEQUEST database search algorithm, and the results were further analyzed for likelihood of interaction using Significance Analysis of INTeractome (SAINT), as well as …
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Complex lipids in microbial mats and stromatolites of Hamelin Pool, Shark Bay, Australia
… and bacteriohopanepolyols were analyzed via liquid chromatography-mass spectrometry (LC-MS) coupled to a Quadropole Time-of-Flight (QTOF) mass spectrometer, while the previously studied fatty acids (Allen et al., 2010) were analyzed using gas chromatography-mass spectrometry (GC-MS) to prove …
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Biochemical Characterization of the Two-component Monooxygenase System; Isobutylamine N-hydroxylase (IBAH) and Flavin Reductase (FRED)
… activity of IBAH was confirmed using liquid chromatography – mass spectrometry (LC-MS). Steady state kinetic data showed an oxidation activity of the monooxygenase component which proceeded at 1.97 ± 0.06 s⁻¹ as measured from oxygen consumption and in product formation, the rate was …
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Effects of Combined Bevacizumab and Paclitaxel On Tumor Interstitial Fluid Pressure In A Preclinical Breast Cancer Model
… Model by Ricardo H. Alvarez Several mechanisms of cell resistance are often accountable for unsuccessful chemotherapy against cancer. Another reason, which has received increased attention, is the inefficient transport of anticancer drugs into tumor tissue. These impaired transports of …
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Cell Wall/Surface Proteome of Candida albicans: its Application in Rapid Identification of Yeast Species by Mass Signature and Characterization by in vitro and in vivo Chemical Labelings
… mechanism of hostpathogen interaction. MALDI-TOF-MS analysis of intact fungal cells yielded mass signatures for rapid species differentiation, strain grouping and yeast morphogenesis monitoring. Cell surface biotinylations at low temperature (4°C), enzymatic digestion of the intact fungal cell …
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Studies of UHPLC-MS performance and application to rapid sensitive and robust drug analysis
… comprises studies of ultra high performance liquid chromatography (UHPLC) performance with a particular focus on the application of liquid chromatography-mass spectrometry (LC-MS) for the analysis of basic drugs. UHPLC-MS technologies are investigated to facilitate fast, sensitive and robust …
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Complex lipids in microbial mats and stromatolites of Hamelin Pool, Shark Bay, Australia
… and bacteriohopanepolyols were analyzed via liquid chromatography-mass spectrometry (LC-MS) coupled to a Quadropole Time-of-Flight (QTOF) mass spectrometer, while the previously studied fatty acids (Allen et al., 2010) were analyzed using gas chromatography-mass spectrometry (GC-MS) to prove …
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Cefazolin Concentration in Surgically Created Wounds Treated with Negative Pressure Wound Therapy Compared to Surgically Created Wounds Treated with Nonadherent Wound Dressings
… and plasma concentrations were measured by liquid chromatography mass spectrometry (LC-MS/MS). Blood samples for measuring plasma cefazolin concentrations were collected prior to biopsy sampling. At the time of surgery and at each bandage change, wound beds were swabbed and submitted for …
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Investigating the therapeutic effects of silver and gold nano particles capped with selected medicinal plants against tuberculosis
… in the plant extracts were profiled using liquid chromatography mass spectrometry (LC-MS). Cytotoxicity activity of plant extracts and nanoparticles were determined by MTS assay on HEK293 cells. The anti-inflammatory activity was determined through the nitric acid synthase (NOS) inhibitory …
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