Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"liquid biopsies"”.
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Label-Free Microfluidic Devices for Single-Cell Analysis and Liquid Biopsies
… contributions towards single-cell analysis and liquid biopsy devices focusing on cancer research. First, cell deformability assays were improved through the introduction of multi-constriction channels, which revealed that cells have a non-linear response to deformation. Combining impedance …
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Genetic Evolution and Prognostic Determinants of Pancreatic Cancer On Longitudinal Liquid Biopsies
… For this reason, we aim to use so called “liquid biopsies” in the form of circulating nucleic acids and exosomes as a strategy that is amenable to longitudinal, relatively non-invasive sampling. Circulating tumor DNA and circulating exosomes contain genetic cargo representative of the …
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Detection of Cell-free Tumor DNA in Liquid Biopsies of Dogs with B cell Lymphoma: A Biomarker Discovery
… assessments. We propose a minimally invasive liquid biopsy technique involving blood collection to detect cell-free DNA from tumors using Next-generation sequencing. We hypothesize that identical tumor aberrations can be found in matching plasma and tumor DNA. Five dogs diagnosed with B-cell …
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Quantitative analyses to study tumor clones dynamics and tumor heterogeneity
… of multiple metastatic and primary tissue biopsies from single patients. I present genomic analyses to decipher the content of multiple tumor biopsies from CRPC patients and provide comparisons to highlight similarities and differences and to identify alternative patterns of aberrations. …
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Amplifying signals in the tumor microenvironment for drug development and diagnostics
… of circulating tumor DNA (ctDNA) molecules in liquid biopsies such as blood. Despite the promise offered by these innovative therapies and monitoring tools, their broad integration into clinical practice faces important challenges. Widespread adoption of emerging therapies demands enhanced …
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Fully Integrated Modular and Mixed-Scale Systems for Processing the Molecular Cargo of Liquid Biopsy Markers
… in nature, like blood or saliva also known as liquid biopsies. Circulating tumor cells, microRNAs, extracellular vesicles (EVs), or proteins are all examples of liquid biopsy biomarkers. Analysis of these biomarkers normally requires a trained operator to run expensive and laborious laboratory …
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Förster Resonance Energy Transfer-Based Quantification of D-2-Hydroxyglutarate & Locked Nucleic Acid Loop-Mediated Isothermal Amplification Mediated Detection of IDH1-R132 Single Nucleotide Variants in Glioma
… fluids suggests its powerful utility in liquid biopsies and monitoring remission vs. recurrence, while the genotyping panel aids in the genetic detection of oncogenic missense mutations. In our hands, both assays have demonstrated 100% accuracy in discriminating between <em>IDH1</em> …
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Identification and Utility of Dna In Exosomes
… diagnosis strategies. Approaches to develop “liquid biopsies” by detecting tumor cells or DNA in the blood have led to several breakthroughs and create the potential for non-invasive, routine assessment of diseases status. However, these biomarkers are rare and currently difficult to isolate, …
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The development of a blood-based test of DNA methylation for the earlier detection of high-grade serous tubo-ovarian cancer
… use of minimally invasive blood sampling, or ‘liquid biopsies’ is a fast-emerging area of cancer diagnostics. Identifying and developing novel DNAme markers for the detection of HGSC in blood samples will fulfil an unmet clinical need in a poor outcome cancer. Methodology A short list of 20 …
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Multi-Modality Plasma-Based Detection of Minimal Residual Disease in Triple-Negative Breast Cancer
… therapy. To this end, I applied the analysis of “liquid biopsies” for early detection of minimal residual disease (MRD) on early-stage TNBC patients using next-generation sequencing. For the first part of this study, I focused on detecting circulating tumor DNA (ctDNA) from TNBC patients after …
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Oncoproteomic applications for detection of breast cancer. Proteomic profiling of breast cancer models and biopsies
… breast cancer cell lines and stage-specific liquid biopsies (nipple aspirate fluid [NAF], plasma samples) to identify disease and phenotype specific signature. An 8-plex iTRAQ quantification strategy was employed to compare proteomic profiles of a range of breast cancer and ‘normal-like’ cell …
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Detecting tumour signals from multi-modal biological features of cell-free DNA fragments
… roadmap for better feature integration in cfDNA liquid biopsies.
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Tools for Monitoring and Modulating Cellular Communication
… constituents at low flow rates. This allows for ‘liquid biopsies’ of the extracellular milieu and yields information on cellular signaling in healthy and diseased states in both in vitro and in vivo models. Better monitoring of the cellular environment elucidates the relationship between proteomic …
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Latent feature models and non-invasive clonal reconstruction
… complicates the molecular interpretation of biopsies, as multiple distinct tumour genomes are sampled and analysed at once. Ignoring the presence of these populations can lead to erroneous conclusions, and so a correct analysis must account for the clonal structure of the sample. Several …
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INVESTIGATING THE ROLE OF EXTRACELLULAR VESICLES AND GLYCOSYLATION IN CANCER METASTASIS
… in cancer and metastasis is often overlooked. Liquid biopsies, including sEV biomarker analysis, offer promising non-invasive diagnostic approaches. Given the prevalence of glycoproteins among blood cancer biomarkers, exploring the glycosylation of sEVs as biomarker targets could revolutionise …
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Investigating the role of extracellular vesicles and glycosylation in cancer metastasis
… in cancer and metastasis is often overlooked. Liquid biopsies, including sEV biomarker analysis, offer promising non-invasive diagnostic approaches. Given the prevalence of glycoproteins among blood cancer biomarkers, exploring the glycosylation of sEVs as biomarker targets could revolutionise …
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Detection of trace levels of circulating tumour DNA in early stage non-small cell lung cancer
Liquid biopsies, using analytes such as circulating tumour DNA (ctDNA), can detect and quantify cancer in a minimally invasive way and can provide information on tumour heterogeneity. Current limitations in the liquid biopsy field are centred around the general sensitivity of the present assays and …
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Developing Tools to Physically Magnify Biological Substrates for Clinical Applications
… nanoscale resolution of clinical pathology and liquid biopsy samples using conventional confocal microscopes. Biology is based on nanoscale building blocks, biomolecules, which interact over nanoscale distances. Diseases, especially in their earliest stages, are associated with subtle changes in …
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The clonal architecture and tumour microenvironment of breast cancers are shaped by neoadjuvant chemotherapy
… in ctDNA in plasma, supporting the use of liquid biopsies as a biomarker for monitoring response to therapy and detection of minimal residual disease.
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Decoding the RNA cargo of plasma-derived extracellular vesicles in metastatic castration-resistant prostate cancer
Liquid biopsies offer a minimally invasive and readily accessible complement to tissue biopsies for monitoring cancer by capturing multiple molecular components circulating in body fluids, such as circulating tumor cells (CTCs), cell-free DNA (cfDNA), cell-free RNA (cfRNA) and extracellular …
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