Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"lipoylation"”.
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Lipoic acid metabolism in Leishmania major
… enzymes that permit one of the two pathways. Lipoylation of the PDH has been demonstrated in a parasite related to Leishmania called Trypanosoma brucei; however there have not been any investigations into the enzymes involved in LA metabolism in either Leishmania or Trypanosoma brucei. In …
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The role of ABHD11 in the regulation of the hypoxia inducible transcription factors
… modification on the OGDHc E2 subunit (DLST), lipoylation, which is sensitive to damage from reactive oxygen species and lipid peroxidation products. Together, these studies identify ABHD11 as a new component maintaining TCA cycle function through regulating the lipoylation of the OGDHc.
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Immunological and biosynthetic studies of the human pyruvate dehydrogenase complex
… hybridomas (IgG/λ) that interact with a common lipoylation-dependent epitope found on both E2 and E3BP lipoyl domains although the precise antigenic determinant has not been fully defined to date. To address this issue, three amino acid residues adjacent to the lipoylated lysine residue of the …
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Rational design and directed evolution of probe ligases for site-specific protein labeling and live-cell imaging
… selection, produced a truncated LplA mutant with lipoylation activity equivalent to full-length LplA. We next sought to evolve activity for an unnatural small molecule probe, but found that this strategy was limited by both increased hydrophobic probe sticking when using the truncated enzyme and …
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Post-Translational Modification With Lipoic Acid in Escherichia Coli
… required for function in vivo of the endogenous lipoylation pathway. However, experiments with purified LipB and LipC show that only LipB is required for the transfer of lipoyl moieties from lipoyl-ACP to apo-proteins in vitro. LipC is highly homologous to Lpd (lipoamide dehydrogenase), the E3 …
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Lipoic acid protein ligases in Plasmodium spp.
… mitochondrion and thus two organelle specific lipoylation pathways are present in these parasites. Biosynthesis of the cofactor occurs in the apicoplast. Octanoyl-[acyl carrier protein]: protein N-octanoyltransferase (LipB) catalyses the attachment of octanoyl-acyl carrier protein …
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Analysis of genetic mutations using a recombinant model of the mammalian pyruvate dehydrogenase complex
… by examining the effects of alterations in its lipoylation status on its activity and overall properties and secondly by assessing the characteristics of recombinant PDC lacking the E3BP subunit. A possible enzymatic role for E3BP in promoting formation of an S6, S8-diacetylated dihydrolipoamide …