Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 70 for “"lipid membrane"”.
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Observations in Lipid Membrane Systems
The plasma membrane is phase separated into a fluid (Ld) phase and a more ordered (Lo) phase. The latter exists as small “rafts” of specific lipid composition containing a host of signaling proteins. Lipid raft theory links the aggregation of rafts to a signaling event. In its current iteration, …
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Rational Design of DNA-Based Lipid Membrane Pores
… In this thesis, we use DNA to build synthetic membrane-inserting channels. Porphyrin and cholesterol tags serve as membrane anchors to facilitate insertion into the lipid membrane. With atomic force microscopy, confocal imaging and ionic current recordings we characterise our DNA nanochannels …
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Spatial and temporal control of lipid-membrane morphology induced by sphingomyelinase
… the cellular processes by reorganizing the cell membrane morphology. This thesis aims at a more comprehensive understanding of how sphingomyelinase (SMase) can regulate lipid membrane heterogeneity. We develop corralled model raft membranes in a microfluidic device to study the complex phase …
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Simulation of lipid membrane models at coarse-grained resolution Towards novel methodologies with improved capabilities
… methodologies for simulating biological membranes at coarse-grained (CG) resolution, combining well-established modeling strategies with recent advances in machine learning. It presents an optimized set of lipid representations and parameters for the Martini 3 CG force field (FF), …
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Scanning Probe Microscopy Study of Molecular Nanostructures on 2D Materials
… and biophysical processes on the biological membrane. Two main techniques of nano-probing are used: scanning tunneling microscopy (STM) and atomic force microscopy (AFM). The study particularly emphasizes on self-assembled molecules on flat 2D materials and quasi-1D wrinkles. First, we report …
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Lipid Chemistry and Mechanical State of the Membrane Modulate Ion Channel Function
… 1945). Kv channel gating is governed by the transmembrane voltage, they are therefore voltage-dependent switches for ionic current (Hille, 2001). Changes in the transmembrane voltage are sensed by the channel's voltage sensor domains, which contain charged amino acids (most often arginines) called …
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Passive transport through biological membranes
… into cells via cellular walls made of lipid membranes. Through the wall the cell needs to transport nutrients, waste, genetic information and signaling molecules. The cell achieves this task via passive and active transport. In this thesis we focus on passive transport processes. In …
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Investigating Cholesterol Orientation in Lipid Bilayer by Raman Spectroscopy
Biological membranes are composed of numerous types of molecules ranging from different sterols to phospholipids to membrane partitioning proteins. While the overall compositions of these various elements are understood in part, how the phospholipid bilayer in cells accommodates the different …
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Biophysical characterization of the Escherichia coli RNA degradosome
… that recruits the degradosome to the cytoplasmic membrane. Investigations on the membrane binding properties of the RNase E as well as the RNA unwinding properties of RhlB were performed in this study. Total internal reflection fluorescent (TIRF) microscopy of the RNA degradosome fluorescently …
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Early Diagnosis of Alzheimer's Disease and Studying Binding to Opioid Receptors using Whispering Gallery Mode Microtoroid Biosensors
… the microtoroids were coated with an artificial lipid membrane doped with GM1-ganglioside receptors to study interactions with the Cholera Toxin subunit B, which is responsible for Cholera disease. In the second study, the surface of microtoroids were functionalized with an artificial lipid …
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A Study of The Effect of Membrane Targeting Antimicrobial Compounds on Ionic Transport Across Lipid Bilayer Membranes
… properties through studying their peptide-membrane interactions. Melimine has previously been shown to reduce the integrity of membranes, observed through the leakage of dye from bacterial membranes. The membrane-peptide interactions were compared using advanced lipid membrane biophysical …
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Dynamics and Structures of Phospholipid Assemblies
… thesis studies structures and dynamics of phospholipids vesicles and their interactions with nanoparticles and peptides, with a focus on the following problems: (1) Single-vesicle dynamics for new colloidal physics. Phospholipids vesicles are stabilized by physically adsorbed nanoparticles, and …
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The Effect of impaired Cyclooxygenase 2 activity on gene regulation in the developing mouse brain and the role of PGE2 in oxidative stress production in Differentiated Neuronal Cells
… (PGE2) is a signaling molecule derived from the lipid membrane through the enzyme cyclooxygenase2 (COX-2). These studies aim to investigate how changes in COX-PGE2 signalling can influence mouse gene expression and alter oxidative stress in neuronal cells. In study 1 we use microarray analysis to …
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Parameterization of the Gay-Berne coarse-grained potential from atomistically detailed anisotropic free energy volumes
… of liquid crystals, protein dynamics and lipid membrane and micelle formation...
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Identifying Modulators of the Development of Acute Functional Tolerance to Ethanol in Caenorhabditis elegans.
… ethanol response. We also examined the role of lipid membrane composition in the response to ethanol. Identification of modulators that are responsible for alcohol-induced responses will provide a greater understanding of the mechanisms that cause alcohol-related diseases.
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Molecular mechanisms of snare assembly and expulsion of intravesicular contents in cell secretion
… it was believed that during cell secretion, membrane-bound secretory vesicles completely merge at the cell plasma membrane resulting in the diffusion of intra-vesicular contents to the cell exterior and the compensatory retrieval of the excess membrane by endocytosis. This explanation made no …
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Theoretical and computational modeling of peptide-lipid bilayer interaction studied by dynamic force spectroscopy
… that investigate different aspects of peptide-lipid membrane interactions. (1) A general theoretical approach is formulated for the quantitative description of the detachment force distribution, P(F), and the corresponding force dependent detachment rate, k(F), of a peptide from a lipid …
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A novel nanoscale delivery system for spatio-temporal delivery of combination chemotherapy
… a nuclear nanoparticle encapsulated within a lipid membrane and is preferentially taken up by the tumor. The nanocell delivers a temporal release of two drugs within the tumor core: the outer lipid envelope first releases an anti-angiogenesis agent, causing a vascular shutdown; the inner …
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Design, Fabrication, and Validation of Membrane-Based Sensors
… looks to develop and characterize a bilayer lipid membrane (BLM) operated artificial hair cell sensor that resembles the stereocilia of the human ear. To develop this sensor, a flexible substrate with internal compartments for hosting the biomolecules and mating cap are constructed and …
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Self-assembly of filopodia-like structures on supported lipid bilayers
… by applying frog egg extracts to supported lipid bilayers containing phosphatidylinositol(4,5)bisphosphate, PI(4,5)P 2. The FLSs recapitulate important characteristics of filopodia - they assemble parallel actin bundles from the lipid membrane and they form in the presence of capping …
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