Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"lipid biosynthesis"”.
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Lipid Biosynthesis and Maturation Signals in Batrachochytrium Dendrobatidis In Vitro
… Confocal microscopy was used to observe lipid biosynthesis and packaging throughout the sessile life stage of the fungus. Maturation assays were conducted to examine the density-dependent maturation of Bd and to isolate potential developmental regulators secreted by zoosporangia that …
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Biochemical characterization of the first step in ether lipid biosynthesis: acylation of dihydroxyacetone phosphate
Glycerolipid biosynthesis is essential for cellular growth and proliferation. It is an indispensable pathway in many living organisms. In mammals four GPAT isoforms are known to catalyze the first step in glycerolipid biosynthesis which involves acylation of glycerol-3-phosphate to lysophosphatidic …
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Plastidial carbonic anhydrase in cotton (Gossypium hirsutum L.): characterization, expression, and role in lipid biosynthesis
… rate of [14C]-acetate incorporation into total lipids in cotton embryos and tobacco leaves and cell suspensions in vivo and in vitro. Similar results were obtained in chloroplasts isolated from leaves of transgenic CA antisense-suppressed tobacco plants (5% of wildtype activity). Collectively, …
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On connections between metazoan cellular metabolism and cell size
… the relative expression of mitochondrial and lipid biosynthesis genes are downregulated with increasing cell size. However, mitochondrial content of the liver samples was not decreased, suggesting that cell functions and cell contents scale differently with cell size. To better investigate how …
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Improving biomass of the marine microalga Nannochloropsis oculata by chemical elicitation
… of particular interest due to their ability to biosynthesise specialised metabolites with a variety of important properties, such as antioxidant and antimicrobial potential. Neutral lipids produced by microalgae are also of huge importance because of their desirable characteristics for the …
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The relationship between docohexanoic acid (DHA) and L-serine, providing an insight into the biochemistry of meningioma
… an altered state of metabolism surrounding lipid biosynthesis. However, only until recently has metabolic reprogramming been a recognised hallmark of the disease. The number of cancer cases diagnosed is set to triple by 2030, demonstrating the need for disease prevention, improved diagnostic …
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Arachidin 3 Modulation of Lipid Metabolism in Rotavirus Infections
… RV replication is dependent on the presence of lipids. This study investigated the alteration of lipid metabolism by A3 in RV infected HT29.f8 cells. A decrease in the RV regulation of lipid biosynthesis genes was observed with the addition of A3 using qRT-PCR. Also, immunofluorescent and …
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The biochemistry of lipid accumulation in Mucor circinelloides and Mortierella alpina
1. The profile and biochemistry of growth and lipid accumulation in M. circinelloides and Mt. alpina were investigated. 2. A nitrogen-limited condition was vital in triggering lipid accumulation in both fungi, which was in agreement with previous work reported in oleaginous yeasts (Botham and …
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Serine Starvation Modulates mRNA Splicing Through Depletion of SR Proteins
… downstream functions in protein, nucleotide, and lipid biosynthesis. There are ongoing clinical efforts to utilize dietary serine starvation as a potential therapy for serine auxotrophic tumors that cannot make their own serine and are dependent on exogenous serine for growth. This emphasizes the …
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Bioaccumulation of Triclocarban, Triclosan, and Methyl-triclosan in a North Texas Wastewater Treatment Plant Receiving Stream and Effects of Triclosan on Algal Lipid Synthesis.
… Arabidopsis have been shown to reduce fatty acid biosynthesis and total lipid content by inhibiting the trans-2 enoyl- ACP reductase. The lipid synthesis pathway effects of TCS on field samples of Cladophora spp. were also investigated in this study by using [2-14C]acetate radiolabeling …
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Examining metabolic vulnerabilities for cancer therapy
… tumors in the brain parenchyma display enhanced lipid biosynthesis, which could guide therapeutic strategies to treat cancer based on tumor site. Collectively, these studies contribute to an understanding of how the reprogrammed metabolism of cancer cells introduces targetable dependencies, with …
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The immunological role of cell wall components from diverse Mycobacterium tuberculosis clinical isolates in regulating HIV-1 replication in human macrophages
… replication potentially due to bioactive Mtb lipids engaging macrophage surface receptors, thus triggering signaling pathways which activate human transcriptional factors (hTF) and production of inflammatory cytokines capable of activating HIV-1 transcription. This work investigated the …
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Expanding the genetic toolbox for methanogenic archaea and investigating ferredoxin redox circuit formation in Methanosarcina acetivorans
… is the primary electron donor for phospholipid saturation in Methanosarcina acetivorans, enhancing our understanding of ferredoxin dependent redox circuits and lipid biosynthesis in methanogens. In Chapter 3, I report the sat gene as a novel positive selectable marker for Methanosarcina …
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Discovery of Enzymes Responsible for an Alternate Mevalonate Pathway in Haloferax volcanii
… in providing the isoprenoids for archaeal lipid biosynthesis. However, the enzymes responsible for mevalonate metabolism in archaea have remained either cryptic or poorly characterized. To identify the enzymes responsible for the mevalonate pathway in Haloferax volcanii, open-reading frames …
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Local and Systemic Consequences of Reducing Notch Signaling in Skin Keratinocytes
… for proper epidermal differentiation and lipid biosynthesis in the epidermis, which in turn lead to formation of a competent skin-barrier. We showed that these functions were executed through both canonical: RBP-j-dependent) and non-canonical Notch signals. Accordingly, we discovered that …
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Novel Therapeutic Targets Identified By High-Throughput Technologies For Triple-Negative Breast Cancer
… induced up-regulation of genes involved in lipid biosynthesis, increased protein expression of oncogenes involved in the MAPK/ERK and DNA repair pathways, and reduced the serum-starvation induction of biochemicals involved in oxidative stress response and glycolytic metabolism. shLRP8 …
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Characterisation of the PQ-loop repeat membrane protein family in arabidopsis thaliana
… as well as defence, cell-surface, lipid biosynthesis and ER stress associated proteins. Finally, the functional importance of the AtPQL family is discussed and a role for AtPQL4 and AtPQL6 in glycosylation proposed.
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REEP5-MFN1/2 tethering in ER-mitochondria crosstalk
… MERCs facilitate critical processes, including lipid biosynthesis, calcium transfer, and mitochondrial dynamics, via specific protein complexes. Dysregulation of MERCs has been implicated in the pathogenesis of several neurodegenerative diseases. Mitofusin 2 (MFN2), proposed to reside on the ER, …
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Investigating the role of ACSS2-mediated acetyl-CoA metabolism on EGFR-PI3K-PKB signalling
… key cellular processes, including *de novo* lipid biosynthesis, energy production and protein acetylation. Whilst there are multiple routes of acetyl-CoA synthesis, acetyl-CoA synthetase short chain family member 2 (ACSS2) is responsible for acetate-dependent synthesis of acetyl-CoA. The …
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Algae and cyanobacteria platforms for engineering biofuel production
… triggers were used to redirect carbon into lipid biosynthesis. Wild-type and starch mutants of Chlamydomonas reinhardtii, a well-established model green alga, were subjected to nitrogen starvation to examine carbon storage and partitioning between starch and lipid synthesis. The starch-less …
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