Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"linker histone H1"”.
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Regulation of Chromatin Multi-Scale Organization and Function by Linker Histone H1
… One factor that appears to play a key role is linker histone H1, which is the most prevalent chromatin-binding protein in the cell. It has long been thought to be responsible for compacting chromatin and repressing transcription, though many historical studies of H1 were performed in vitro, and …
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CRAMP1 drives linker histone expression to enable Polycomb repression
The essential role of the core histones (H2A, H2B, H3 and H4) in DNA packaging has been well-understood for decades, but the function of the linker histone (H1) remains enigmatic. H1 compacts nucleosome arrays in vitro, but the multiple H1 subtypes and variants encoded in mammalian genomes (11 in …
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Investigations into the role of histone H2A ubiquitination in chromatin
… vitro system was used to determine the effect of histone H2A ubiquitination on linker histone binding to mononucleosomes. Hybrid octamers containing either H2A or ubiquitinated H2A (uH2A) were reconstituted onto random sequence 167 bp DNA. The affinity of the resultant nucleosome cores for linker …
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STRUCTURAL INSIGHTS INTO RNA POLYMERASE III PRE-INITIATION COMPLEX AND THE PIONEER ROLE OF OCT1
… superhelical locations that engage and remodel histone H4 tails. These interactions reposition histone tails and displace linker histone H1, resulting in chromatin destabilization and increased DNA accessibility. Altogether, this thesis provides structural and mechanistic insights into …
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Novel Roles of the Aryl Hydrocarbon Receptor in Normal Physiology and Epigenetic Reprogramming
… CPS recruitment results in homocitrullination of linker histone H1 lysine 34 (H1K34hcit). Homocitrullination, a novel epigenetic mark, was observed only in response to exogenous AhR agonists and is absent upon treatment with endogenous AhR ligands such as cinnabarinic acid. Results presented here …
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Autoimmune Consequences of Histone Deimination during Neutrophil Activation
… requires the post-translational modification of histones and results in the extracellular release of chromatin. The extracellular chromatin incorporates histones in which arginines have been converted to citrullines by peptidylarginine deiminase IV (PAD4) creating structures that capture or …
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Structural and biochemical studies of the yeast linker histone, Hho1p
… pairs of DNA wrapped around an octamer of core histone proteins. Linker histones bind through their globular domain at the nucleosome dyad and to internucleosomal DNA through their C-terminal basic tail. The Saccharomyces cerevisiae linker histone homologue, Hho1p, contains two domains, GI and …
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Mechanical Manipulation of Eukaryotic Chromatin by DNA-Binding Proteins
… I characterize the dynamic interplay of Sox2 and H1 DNA binding activity. Both families constitute large classes of chromatin and DNA binding proteins that have been historically thought to be antagonistic regulators of each other, but the underlying mechanism is not well understood. Using …
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Single-Molecule Investigation of Chromatin-Associated Factors in Genome Organization and Epigenetic Maintenance
… thought that PRC2 propagates repressive histone marks by modifying neighboring nucleosomes in a strictly linear progression. However, the behavior of PRC2 on native-like chromatin substrates remains incompletely characterized, making the precise mechanism of PRC2-mediated heterochromatin …
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Characterization of the Genes Essential for Mouse Spermatogenesis
… another spermatid-specific gene, which encodes a linker histone H1-like protein. The expression of HILS1 overlaps with the expression of transition nuclear proteins (TNP1 and TNP2). While <italic>Hils1<super>-/-</super></italic> males were fertile and <italic>Tnp1<super>-/-</super></italic> males …
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Making Molecular Movies: Using Single-Molecule Techniques to Unveil Hidden Features of Protein-Chromatin Interactions
… we report a new method that utilizes the histone chaperone Nap1 to directly assemble nucleosomes on DNA substrates stably tethered within a smCFFM instrument. In addition to more closely resembling the physiological pathway of nucleosome formation in vivo, this method allows users to …