Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"linker histone"”.
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CRAMP1 drives linker histone expression to enable Polycomb repression
The essential role of the core histones (H2A, H2B, H3 and H4) in DNA packaging has been well-understood for decades, but the function of the linker histone (H1) remains enigmatic. H1 compacts nucleosome arrays in vitro, but the multiple H1 subtypes and variants encoded in mammalian genomes (11 in …
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Linker Histone Medicated Regulation of Mitotic Chromosome Compaction and Individualization
… a mystery. Here, I investigated the role of the linker histone variant H1.8 in regulating mitotic chromosome structure. I showed that H1.8 suppresses binding of condensins and topo II to mitotic chromatin in Xenopus egg extracts. Using an in vitro reconstitution system, I showed that H1.8 …
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Structural and biochemical studies of the yeast linker histone, Hho1p
… pairs of DNA wrapped around an octamer of core histone proteins. Linker histones bind through their globular domain at the nucleosome dyad and to internucleosomal DNA through their C-terminal basic tail. The Saccharomyces cerevisiae linker histone homologue, Hho1p, contains two domains, GI and …
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The role of linker histone variant in double strand break repair
… to access the DNA. It has been shown that linker histone variants inhibit the double strand break (DSB) repair pathway known as non-homologous end joining (NHEJ). NHEJ, in vivo, requires the KU complex, the DNA dependent protein kinase (DNA-PK) and the DNA ligase IV/XRCC4 (LX) complex. In …
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Regulation of Chromatin Multi-Scale Organization and Function by Linker Histone H1
… One factor that appears to play a key role is linker histone H1, which is the most prevalent chromatin-binding protein in the cell. It has long been thought to be responsible for compacting chromatin and repressing transcription, though many historical studies of H1 were performed in vitro, and …
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An experimental approach to determine the binding mode of yeast linker histone, Hho1p
… to investigate of the binding mode of yeast linker histone Hholp to nucleosomes, using magnetic bead pull-down assays. To understand the structural and regulatory role of, Hholp, the linker histone in Saccharomyces cerevisiae, and that of higher eukaryotes (Thoma et al., 1979), it is …
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The association of the secondary DNA-binding site of linker histone H5 in a nucleosome
In order to understand the role of linker histones in the formation of the 30-run chromatin fibre as well as their role in transcriptional repression, it is essential to know their location on the nucleosome. In this study, we have modelled the location of the globular domain of chicken linker …
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Dissecting the Architectural Properties of Chromatin and the Influence of the H1 Linker Histone on Chromatin Regulation
… Collepardo Lab to study the binding of the H1 linker histone to chromatin and delve into the intrinsic properties of chromatin that regulate its architecture. The primary aim is to decipher the factors modulating chromatin organisation using a chemically-specific molecular model. I first …
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The phosphorylation state of Saccharomyces cerevisiae linker histone Hho 1p during entry and exit of stationary phase
Our group has recently found that the linker histone Hh01 p of Saccharomyces cerevisiae exhibited a significant increase in binding to chromatin during stationary phase. Because of the role of H1 in gene expression and chromatin compaction, it is essential to understand the mechanism behind this …
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Investigations into the role of histone H2A ubiquitination in chromatin
… vitro system was used to determine the effect of histone H2A ubiquitination on linker histone binding to mononucleosomes. Hybrid octamers containing either H2A or ubiquitinated H2A (uH2A) were reconstituted onto random sequence 167 bp DNA. The affinity of the resultant nucleosome cores for linker …
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Genomic distribution of histone H1 in budding yeast (Saccharomyces cerevisiae) : yeast chromosome III
The linker histone HI binds to the nucleosome and is essential for the organization of nucleosomes into the 30-nm filament of the chromatin. This compaction of DNA has a well-characterized effect on DNA function. In Saccharomyces cerevisiae, HHO 1 encodes a putative linker histone with very …
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In silicio Streckung und Kompaktierung von Chromatin
… Repeatlänge, des Öffnungswinkels oder vorhandene Linker-Histone zu steiferen Fibern führen. Persistenzlängen und Streckmodule stimmen mit experimentellen Daten überein. Schließlich demonstriere ich, dass die Chromatinfiber wesentlich widerstandsfähiger gegenüber der Dehnung als gegenüber der …
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Compaction of chromatin in Saccharomyces cerevisiae
… the link between the association of the yeast linker histone homologue, Hholp, and the compaction of the yeast genome during stationary phase. The relative gene content of condensed chromatin, fractionated and isolated by sucrose gradient ultracentrifugation from stationary and exponential …
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A role for Hho1p, a histone H1 homologue, in Saccharomyces cerevisiae
It is well established that histone H1 is involved in facilitating the higher-order structures of chromatin. It has not become possible to test the biochemical function of H1 in Saccharomyces cerevisiae, since the identification of a H1 homologue (Landsman, 1996) with properties of a bona fide …
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Faktoren des Kerntransports von Core-Histonproteinen: Strukturelle und funktionelle Analyse
Core-Histone sind kleine basischen Proteine, die die Nukleosomen im Chromatin eukaryonter Zellen bilden. In vitro Kernimportstudien zeigen, daß die vier Core-Histone H2A, H2B, H3 und H4 nicht durch freie Diffusion, sondern durch einen aktiven, rezeptorvermittelten Prozeß in den Zellkern importiert …
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Deriving Structural Insights About Mesoscale Chromatin Folding Using Coarse-Grained Oligonucleosome Modeling
… patterns of fiber compaction as a function of linker DNA length. Using an exponential model of spatially correlated cleavage by ionizing radiation, we predict RICC-seq fragment length distributions from simulated chromatin structure ensembles to determine the correspondence between chromatin …
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Palmitic Acid Activation of Dendritic Cells: Implications for Type 2 Diabetes
… is regulated in part by a major reduction of linker histone proteins. In adipose tissue, dendritic cells are exposed to high levels of saturated fatty acids. Increased saturated fatty acid levels derived from high fat diets are strongly associated with chronic adipose tissue inflammation. …
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Reexamining the role of linker histones beyond 30 nm fibers in a complex chromatin environment
… primary players in chromatin compaction are core histones, which bind DNA to form the nucleosome and the basis for 10 nm fibers. Linker histones also play an important role in chromatin compaction. Previous work showed that linker histones are important for the formation of 30 nm structures. 30 nm …
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Biochemical and structural studies of histone and associated proteins from chick and human nuclei
Chromatin is the complex of DNA, histones and non-histone proteins that make up chromosomes and the region of dispersed chromosomes during the interphases and S phase of the cell cycle. It is found inside cell nuclei in eukaryotic cells. There are many important proteins in the nuclei which are …
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Licensing Of Cell Fate Reprogramming Via Leafy Transcription Factor Pioneering Activity
… enriched binding sites, LFY displaces the H1 linker histone and recruits SWI/SNF chromatin remodelers. The combined data suggest that the pioneer transcription factor LFY acts as a licensing factor for floral fate that facilitates binding of additional non pioneer transcription factors, …
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