Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 97 for “"lineage tracing"”.
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Single-Cell Lineage Tracing Of Cancer Metastasis
… macsGESTALT, an inducible CRISPR-Cas9-based lineage recorder with highly efficient single-cell capture of both transcriptional and phylogenetic information. Applying macsGESTALT to a mouse model of metastatic pancreatic cancer, we recover ~380,000 CRISPR target sites and reconstruct …
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Identification, regulation and lineage tracing of embryonic olfactory progenitors
… and central nervous system (CNS) progenitors and lineage trace their progeny, our spatial and temporal understanding of embryonic OE neuroglial progenitors has been stalled by the paucity of identifiable genes able to distinguish individual candidate progenitors. In the developing CNS, radial glia …
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Lineage tracing of normal human development and childhood cancers
… normal tissues can shed light on the embryonic lineage of tumours and may reveal the precise juncture at which tumours began to form. Accordingly, I studied the origin of Wilms tumour, the most common childhood cancer of the kidney. I discovered that these tumours often arise from large …
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Lineage tracing of Ascl1-expressing cells in the maternal liver during pregnancy
… maternal hepatic Ascl1-expressing cells and (2) tracing the fate of Ascl1-expressing cells in the maternal liver during pregnancy. Timed pregnancies were generated and non-pregnant (NP) and pregnant maternal livers were harvested and analysed. To identify the maternal hepatic Ascl1-expressing …
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Computational Approaches to Understand Chemoresistance & Tumor Evolution Using Longitudinal Clinical Data and Lineage Tracing
… developed a novel platform using single-cell lineage tracking (clonal replica tumors - CRTs), which enabled us to model and dissect functional heterogeneity in early passage PDAC patient-derived xenografts (PDX) models. These models were used to identify the fitness landscape of individual …
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Probing Steady-state and Post-transplant Blood System Dynamics with Computational Analysis and Lineage-tracing
… samples. We also discuss using clonal lineage tracing to track cell clones throughout the transplant process and present some preliminary analyses.
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MULTI-OMIC LINEAGE TRACING PREDICTS THE MOLECULAR DRIVERS OF CANCER STEM CELLS IN TRIPLENEGATIVE BREAST CANCER EVOLUTION.
… a single-cell multiomic framework that combines lineage tracing with transcriptomic and chromatin accessibility profiling (via snRNA-seq and snATAC-seq) and integrates these with phenotypic assays. Applying GALILEO to the intrinsically heterogeneous and highly tumorigenic TNBC cell line SUM159PT, …
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A Characterisation and Understanding of the Germinal Centre B Cell Response to Atherosclerosis in Mice using Lineage Tracing
… The tamoxifen-inducible AID-CreERT2-Rosa-EYFP lineage tracing mouse model was used and crossed with the atherosclerosis-prone LDLr-deficient mouse model. This model inducibly and specifically labels germinal centre B cells and their progeny using tamoxifen allowing for tracking of …
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Exploring the role of BCL11A in the oesophagus and mammary gland using in vivo lineage tracing and 3D organoid models.
… this work reports the combined use of in vivo lineage tracing techniques and in vitro 3D organoids to explore the expression and gene function of Bcl11a in the oesophageal epithelium and mammary gland. Gene function studies were undertaken using mouse models of BCL11A overexpression: …
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LINEAGE TRACING REVEALS PAEP AS A CHEMOTHERAPY-INDUCED METASTATIC DRIVER AND EXPOSES METABOLIC VULNERABILITIES OF CIRCULATING TUMOR CELLS IN BREAST CANCER
… residual disease in patients. High‐complexity lineage tracing was combined with single‐cell RNA‐seq to track clonal and transcriptional dynamics across PT, lung metastases, and CTCs. I further developed a high‐throughput workflow to isolate large numbers of viable CTCs from metastasis‐bearing …
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LINEAGE TRACING ANALYSIS OF PANCREATIC ACINAR CELL DE DIFFERENTIATION AND TRANS-DIFFERENTIATION IN VITRO AND IN VIVO IN THE ABSENCE OF MIST 1
… Mist1'Acultures are acinar in origin, molecular lineage tracing analysis was performed in which the R26R LacZ gene was permanently activated in acinar cells. The outcomes demonstrate that deletion of MIST1 enhances the trans-differentiation of acinar cells to other pancreatic cell types, such as …
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The origin and properties of pro-oncogenic fields in the intestinal epithelium
… into the absorptive or secretory cell lineages. However, intestinal cell-fate specification is not always restrictive and early secretory progenitors can revert back to the stem cell pool. Human colon cancer develops by acquisition of oncogenic mutations in the colonic epithelium …
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Stem and Progenitor Cells in Homeostatic Maintenance and Perturbation of the Pulmonary Epithelium
… development. As such, I generate a novel lineage tracing mouse line, Red2-PIK3CAH1047R, which allows for the simultaneous lineage tracing of PIK3CAH1047R mutant cells and tissue-sharing wild-type cells. I then initiate recombination of the reporter in a starting population of basal cells, …
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Cochlear hair cell regeneration from neonatal mouse supporting cells
… cells has been unclear. Here, using in vitro lineage tracing with the supporting cell markers Sox2 and Lgr5, we show that Lgr5-positive inner pillar and 3rd Deiter's cells in gentamicin-damaged organs of Corti from neonatal mice give rise to new hair cells following treatment with a Notch …
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Inferring Clonal Dynamics in Blood using Single-Cell Measurements
… inducible MPN disease and CRISPR array repair lineage tracing (CARLIN), using single-cell RNA sequencing. Our gene expression analysis shows that the mutation keeps key signaling and stress-response genes switched on and boosts growth-promoting enzymes, collectively pushing blood production …
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Elucidation of mammary gland development: combination of novel techniques to study proliferation and stem cells
… to be achieved. A number of recent conflicting, lineage tracing studies have served only to fuel the fires of controversy, with previous characterisation of the mammary gland largely carried out using two-dimensional tissue sections. However, in order to fully appreciate the capacity of MaSCs and …
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Development and Evolution of the Skull-Neck Boundary: Insights from Amphibian Model Species
… in A. mexicanum and X. laevis using cell-lineage tracing techniques to track somitic contributions to the skull, hypoglossal nerve complex morphological data, and traditional whole-mount cartilage and bone staining techniques. Finally, I perturbed Hox gene expression patterns to produce …
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Mosaic Analysis with Double Markers (MADM) as a Method to Map Cell Fates in Adult Mouse Taste Buds.
… either cell cycle gene expression properties or lineage tracing of precursor cells. In our study, we apply a new fate mapping technique that combines these two ideas. This technique, Mosaic Analysis with Double Markers, allows for simultaneous gene knockout and subsequent tracking of single …
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Identification of novel gastric stem cell markers and investigation of Intestinal Stem Cell Cre-mediated Recombination
… stem cells, utilizing Cre/Lox technology for lineage tracing. The recombination efficiencies of reporters with intestinal drivers and the effect of tamoxifen-induced tissue damage were also investigated.</p> <p>It was discovered that higher doses of tamoxifen do not increase reporter …
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