Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 50 for “"lineage specification"”.
-
Stochastic gene expression during lineage specification of single T helper lymphocytes
… the differentiation of which requires numerous lineage specifications at various developmental stages. The precise control of immune cell differentiation and the delicate balance of their population composition are crucial for effective protection against infectious environmental agents, without …
-
Investigating Adult Stem Cell Lineage Specification in the Freshwater Flatworm Schmidtea mediterranea
… of the early temporal window of epidermal lineage progression.
-
TET1 AND TRANSCRIPTION FACTOR REGULATION OF LINEAGE SPECIFICATION DURING MOUSE GASTRULATION AND EARLY ORGANOGENESIS
… day (E) 6.25 and E9.5 in mouse that involve the specification of pluripotent cells into the lineage- and tissue-specific precursors of the adult organism. The dozens of cell types that arise during this period are defined by distinct transcriptional programs that depend on specific remodelling of …
-
Lineage specification of ovarian theca cells requires multi-cellular interactions via oocyte and granulosa cells
… a highly orchestrated process involving multiple lineage determinations of ovarian surface epithelium, granulosa cells, and theca cells. While the sources of ovarian surface epithelium and granulosa cells are known, the embryonic origin(s) of theca stem/progenitor cells have not been definitively …
-
Germ lineage specification from a pluripotent primitive ectoderm-like substrate: a role for cell-cell contacts.
… primitive ectoderm cells form the three germ lineages, mesoderm, ectoderm and endoderm under the control of complex signalling and environmental cues. This process can be modelled using embryonic stem cells, which have proven to respond to embryologically relevant signals during in vitro …
-
Development of an Interferon Bioassay and Primitive Endoderm Cell Lines to Study Lineage Specification During Early Bovine Embryogenesis
… interest to this laboratory are the first two lineage specification events, trophectoderm (TE) and primitive endoderm (PrE) specification, occurring between days 6 and 8 of gestation. The TE is responsible for uterine attachment and production of interferon-tau (IFNT), the factor of maternal …
-
Intrinsic Specificity of Binding and Regulatory Function of Class II bHLH Transcription Factors
… factors which play crucial roles in lineage specification in the developing embryo. In vivo, these factors bind to distinct genomic sites, and regulate distinct transcriptional programs. The mechanisms by which they select their cognate binding sites remain poorly defined. Here, we …
-
The NuRD complex regulates chromatin dynamics driving exit from naïve pluripotency
… gene expression in naive pluripotency through to lineage specification. Single-molecule tracking of pluripotency-associated TFs in live mouse ESCs revealed that remodelling by NuRD acts to actively promote the dissociation of these TFs from chromatin, limiting their residence times. The regulation …
-
T-cell Receptor CDR3 Sequence but Not Recognition Characteristics Distinguish Autoreactive Effector and Foxp3+ Regulatory T-cells
… in responsiveness were not responsible for lineage specification. Notably, autoreactive Treg and Tconv cells could be fully distinguished by an acidic versus aliphatic variation at a single TCR CDR3 residue. Our results imply that ontogenically distinct Treg and Tconv cell repertoires with …
-
Development of a High-Throughput Human iPSC Chondrogenesis Platform and Applications for Arthritis Disease Modeling
… activation might be involved in chondrogenic lineage specification of hiPSCs. Taken together, these studies describe the generation of a high-throughput system for chondrogenesis and its application for screens and arthritis disease modeling. Future applications of this platform may be useful …
-
ELUCIDATING REGULATORY NETWORKS PROMOTING B-CELL DEVELOPMENT
… of the transcriptional network orchestrating B-lineage specification and commitment. Based on the combined expression of surface markers and transgenic reporter genes, we have identified three hierarchically related and functionally distinct subpopulations within the common lymphoid progenitor …
-
Multi-omics characterisation of mouse gastrulation and organogenesis at single-cell resolution
… the mammalian embryo needs to instruct the specification of the epiblast precursors required for the formation of the foetal tissues. At the exit from pluripotency, global epigenetic and transcriptional remodelling occurs. These changes are essential for gastrulation, the process by which …
-
Downregulation of Pax2 Suppresses Ovarian Cancer Cell Growth
… proliferation, oncogenic transformation, cell-lineage specification, migration, and survival. Unattenuated <em>PAX2</em> has been found in several cancer types. We therefore sought to elucidate the role of PAX2 in ovarian carcinomas. We found that PAX2 was expressed in low-grade serous, clear …
-
Mechanisms of CD4+ Regulatory T Cell Heterogeneity in Health and Disease
… factor BACH2 is critical for early Treg cell lineage specification, however, its function following Treg lineage-commitment is unresolved. The studies detailed herein demonstrate that Bach2 is highly expressed during Treg cell development in the thymus. High levels of Bach2 are maintained in …
-
The dissection of VEGFA stimulus-responsive regulatory and transcriptional changes in angiogenesis
… regions, as well as their role in cellular lineage specification. However, these studies generally focused on steady-state cellular states where much of the lifespan of adult cells involves responding to extracellular cues. To better understand the gene expression changes that occur in …
-
Cellular plasticity in white adipose tissue: in vivo identification of bipotent adipocyte progenitors in adult white adipose tissue
… remains poorly understood. Utilizing genetic lineage tracing techniques in combination with in situ imunohistochemical analysis, we demonstrate that brown adipocytes (BA) that are induced by beta3-adrenergic receptor activation (ADRB3) in WAT arise from the proliferation and differentiation of …
-
Role of Notch Signaling and GATA-3 in Establishing T-lineage Commitment and Survival in Developing Thymocytes
… is required to commit progenitors to the T-lineage and acts in concert with many transcription factors such as GATA-3 to fully induce this fate choice while inhibiting the B-cell lineage. Although both Notch and GATA-3 are indispensible for thymocyte development, their precise roles in …
-
Mechanotransduction at the nuclear envelope: the role of forces in facilitating embryonic stem cell fate decisions
… to the nuclear envelope would restrict access to lineage specific genes sequestered at the nuclear lamina and thereby either impair, or delay, differentiation. We found phenotypes in the capacity of mouse ES cells to specify to the neural lineage following our perturbations: overexpression of …
-
CRAMP1 drives linker histone expression to enable Polycomb repression
… of both histone H1 and H3K27me3 changes during lineage specification, eventually resulting in co-localisation in mature neurones. I conclude that linker histones are not a general feature of repressed chromatin but rather are uniquely required for epigenetic repression by PRC2 in differentiated …
-
Establishment of the epigenetic landscape in mammalian embryonic stem cells
… required of stem cell differentiation and lineage specification.
Page 1 of 3