Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"light sheet microscope"”.
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Validation of a Confocal Light Sheet Microscope using Push Broom Translation for Biomedical Applications
… deep chamber of the R-MHD device, an epitaxial light sheet confocal microscope (e-LSCM) was used to improve axial resolution. The linear sensor, having small pixels, blocked out-of-plane light while eliminating the need for a mechanical aperture which is used for traditional point-scanning …
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Light-sheet microscopy used for tracking particles
… has generally been limited to 2D culture dishes. Light-sheet microscopy, a recent advance which was awarded Nature Method of the Year in 2014, allows fast, non-invasive 3D imaging across an entire organism. This works by decoupling illumination and detection such that the microscope only …
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Development of optical systems and imaging analyses for hyperspectral microscopy and 3D super-resolution imaging
… a high-speed hyperspectral line-scanning microscope (HSM) that is capable of recording 128 spectral channels in 27 frames per second. To reduce the aberrations of the spectral images, a spherical prism spectrometer is implemented in HSM. The design and calibrations of the spectrometer are …
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Imagining the Voltage of Neurons Distributed Across Entire Brains of Larval Zebrafish
… thesis presents a high-speed remote scanning light-sheet microscope capable of imaging GEVI-expressing neurons distributed throughout entire brains of larval zebrafish at a volumetric rate of 200.8 Hz. The microscope combines remote refocusing and an ultrafast dual-camera system to …
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Methods for optical microscopy beyond conventional limits
… resolution necessarily entails a larger light dose. This thesis proposes methods for increasing the resolution of fluorescence microscopy beyond that of the widely employed confocal microscopy while reducing photodamage and phototoxicity. Measurements of the structures of fluorescent …
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Super-resolved volumetric optical imaging of virus-infected cells
… them hard to visualise using diffraction-limited light microscopes. Yet, ‘seeing viruses’ is essential to unravel their infection mechanism within the organisms they highjack, which is key to understanding how such small entities can spark disruptive pandemics. This thesis shows how expansion …
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Development of novel imaging technology to study cell signalling
… SPIM using a reflective atomic force microscope cantilever to create a 2 μm thick light sheet. Using this setup, we demonstrated that ldSPIM can deliver single fluorophore labelled proteins onto the plasma membrane of HEK293 cells or into the cytoplasm and characterise the interaction …
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Augmenting Wiring Diagrams of Neural Circuits with Activity in Larval Drosophila
… pan-neuronal calcium indicators is imaged in a light sheet microscope, which are then structurally imaged with high throughput electron microscopy. Methods and tools are provided for the assembly of these image volumes, spatial registration between imaging modalities, automated detection of …
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Volumetric Imaging Across Spatiotemporal Scales in Biology with Fluorescence Microscopy
… The first section details the design of a light sheet microscope capable of high-throughput imaging of cleared, macroscopic samples with cellular resolution. In light sheet microscopy, the combination of spatially confined illumination with widefield detection enables multi-megapixel …
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Shedding light on the molecular interactomes of fast, complex biological processes using multispectral imaging with uncompromised spatiotemporal resolution
… microscopy have enabled the field to shed light on the spatiotemporal nature of fundamental intracellular processes. However, despite these advances, due to the broad emission spectra of conventional fluorophores only three or fewer fluorescently-labelled structures can be observed in live …