Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 4 of 4 for “"ligase detection reaction"”.
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Fabrication of DNA microarrays on Poly (methylmethacrylate) substrates for biomolecular reporting
… value for colorectal cancers whereby a Ligase Detection Reaction/universal zipcode array assays was carried out using parallel detection of two different low abundant DNA point mutations in KRAS 2 oncogenes with allelic composition evaluated at one locus. The same covalent attachment …
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Single-molecule detection of molecular beacons generated from LDR on thermoplastic microfluidic device for bioanalysis
Current clinical techniques for nucleic acid detection and analysis often involve PCR, lacking adequate specificity or sensitivity to meet the stringent requirements in certain applications. This research aims to develop an innovative molecular assay and the associated hardware to rapidly signal …
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Genome analysis: mutation analysis using near infrared laser-induced fluorescence (NIR-LIF) and single molecule detection in microfluidic devices
… DNA amplification via polymerase chain reaction (PCR) provides an unlimited supply of material for subsequent genetic analysis even in case where only a single copy of the DNA molecule is present in the sample. Research presented in this dissertation first examines the efficiency of in …
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Advancing Technologies for Precision Medicine: From Liquid Biopsy to Single-Molecule DNA Sequencing
… rat sarcoma) mutations were detected using a ligase detection reaction assay. These analyses demonstrated that CTC-derived DNA can capture the mutational profile of the primary tumor and enable the detection of variants in the bloodstream, allowing the tracking of mutations gained or lost …