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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 552 for “"ligase"”.
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The Synthesis of Oligodeoxyribonucleotides With Rna Ligase
RNA ligase, isolated from bacteriophage T4-infected Escherichia coli catalyzes the ATP-dependent formation of a 3' (--->) 5'-phosphodiester bond between one oligonucleotide with a 3'-hydroxyl group (the acceptor molecule) and another with a 5'-phosphate (the donor molecule). The enzyme does not …
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Characterization of SCF Ubiquitin-Ligase Subunits in Arabidopsis
… the formation of the SCF-class of E3-ubiquitin ligase complexes. One such gene family encodes the Skp1-like class of polypeptide subunits, where 21 genes have been identified and are known to be expressed in Arabidopsis. The complexity of this family of Arabidopsis Skp1-like--or ASK --genes, …
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Regulation of Apoptosis by XIAP Ubiquitin- Ligase Activity
… RING domains that function as E3 ubiquitin-ligases to regulate the abundance of IAPs themselves and their binding partners by engaging the ubiquitin system. The precise physiological function of individual mammalian IAPs and their role as E3- ubiquitin ligases in situ remain largely obscure. …
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Intermolecular Joining of Dna Oligonucleotides With Rna Ligase
Made available in DSpace on 2014-12-14T04:27:48Z (GMT). No. of bitstreams: 1 8009101.pdf: 6452301 bytes, checksum: 2b7980a57152a1c82231d5c8a4574206 (MD5) Previous issue date: 1979
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Modification of Transfer Rna With T4 Rna Ligase
T4 RNA ligase can be used to alter the sequence of transfer RNAs. This enzyme will catalyze the addition of a 3',5'-bisphosphate onto the 3' terminus of a tRNA resulting in a tRNA molecule one nucleotide longer with a 3' phosphate. Under appropriate conditions the reaction is quantitative and, if …
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Characterization of The Ubiquitin Ligase, Ube4B, In Endocytic Trafficking
… interaction between UBE4B, an E3/E4 ubiquitin ligase, and the ESCRT-0 components, Hrs and STAM. ESCRT-0 is the first complex of the endosomal sorting machinery to bind to endosomes, and recognition by ESCRT-0 is required for EGFR sorting and degradation. Immunoprecipitation and ubiquitination …
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Ubiquitin E3 ligase mediated regulation of HMG-CoA Reductase
… with Insig proteins, which recruit E3 ubiquitin ligase complexes to mediate degradation of HMGCR by the ubiquitin proteasome system. However, the identity of the E3 ligase(s) responsible for HMGCR ubiquitination is controversial. Here, I use a series of genome-wide CRISPR knockout screens using a …
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Insights into the regulation of the CRL4D̳T̳L̳ ubiquitin ligase
… and orderly manner. Cullin E3 ubiquitin ligases (CRLS) comprise a large family of multi-subunit complexes that selectively recruit substrates via a substrate receptor and facilitate substrate ubiquitination and degradation. The CRL4DTL (Cullin 4 RING ligase, in association with the …
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Design and Synthesis of an E3 ligase Activity-Based Probe and its Application for the Discovery of a New Class of E3 Ligase
… target for drug development. However, E3 ligases (E3s) represent an important class of enzymes yet there are currently no effective tools for profiling their activity. Herein, a new class of E3 activity-based probe (ABP) is presented which is built by re-engineering ubiquitin (Ub)-charged …
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Roles for the E3 Ligase Itch in Immune Response Modulation
… the agouti mutant strain a has implicated the E3 ligase Itch in the spontaneous inflammation that occurs in these mice. 1 RH Anaylsis of a mice suggests that the inflammatory disease is due to a dysregulated T helper type-2 immune response. In order to verify the importance of Itch inactivation in …
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Investigating the protein targets of the neuroprotective E3 ligase, CHIP
… is the dual function chaperone and E3 ligase C-terminus of Hsc-70 interacting protein (CHIP). Its role in neurodegenerative diseases, including dementia with Lewy bodies, and the severe progeria and proteotoxic phenotype seen in CHIP KO mice support its neuroprotective effects, but the …
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Regulation of the Parkin E3 ligase by PINK1-dependent phosphorylation
… Parkin encodes a RING-In Between RING-RING E3 ligase that normally resides in the cytoplasm and is<br/>regulated by autoinhibitory intramolecular interactions. PINK1 is a mitochondrial Ser/Thr kinase that is unique in possessing a N-terminal mitochondrial targeting sequence and three loop …
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The Phosphatase Activities of T4 Polynucleotide Kinase (Rna Ligase, Splicing)
… and kinase activities function with RNA ligase in tRNA breakage and religation.
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Investigations of peptide aminoacyl tRNA ligase (PEARL) biosynthetic gene clusters
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-12-01
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STRUCTURAL INSIGHTS INTO POLYUBIQUITIN CHAIN FORMATION MEDIATED BY NEDD4 HECT LIGASE
… founding member of a family of HECT E3 ubiquitin ligases which regulates the trafficking and stability of receptors and signaling proteins. Precise spatiotemporal control of its activity is essential for orchestrating cellular functions. In resting condition, NEDD4 N-terminus by interacting with …
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바이러스 감염시 미토콘드리아 E3 ubiquitin ligase 인 MARCH5의 새로운 생체 기능
… investigated functional role of mitochondrial E3 ligase, MARCH5, against protein aggregated at the mitochondria membranes. First, the mitochondrial antiviral signaling (MAVS) protein forms aggregates that elicit robust type-I interferon induction on viral infection, but persistent MAVS signaling …
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Nucleotide recognition and metal ion requirements of an RNA ligase ribozyme
The class I ligase, a ribozyme previously isolated from random sequence, catalyzes a reaction similar to RNA polymerization, positioning its 5'-nucleotide via a Watson-Crick base pair, forming a 3',5'-phosphodiester bond between its 5'-nucleotide and the substrate, and releasing pyrophosphate. The …
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The Role of HOIL 1 Ubiquitin Ligase Activity in MDA5 Antiviral Signaling
… work had identified that the E3 ubiquitin ligase, HOIL1, is essential for the induction of type 1 interferons in response to viral infections primarily sensed by MDA5 but is dispensable for RIG-I mediated interferon induction. However, these findings were confounded by the role of HOIL1 as …
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Investigations into the Biological Roles of the E3 ligase Ariadne 2/TRIAD1
… immune responses. Ariadne 2 is a RING finger E3 ligase and is part of the highly conserved RBR (RING-B-Box-RING) superfamily, however, little is known of its function in mammalian systems. To further examine the physiological role, Ariadne 2 deficient mice were generated. In a mixed background, …
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Expanding the Scope of T3 DNA Ligase-Catalyzed Oligonucleotide Polymerizations (T3-LOOPER)
… can be incorporated into one polymer. Ligase-catalyzed oligonucleotide polymerization (LOOPER) is a method that synthesizes chemically modified nucleic acid libraries containing up to 16 unique chemical modifications. The method entails template directed ligations of chemically modified …
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