Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"let-7"”.
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The Functional Roles of the Lin28/let-7 Axis in Tissue Regeneration and Cancer
The let-7 microRNAs and their antagonists, the Lin28 RNA-binding proteins (Lin28a and Lin28b), are post-transcriptional regulators well known for their initially discovered role in controlling developmental timing, or heterochrony. Subsequent studies have further uncovered many additional cellular …
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A Platform for Fast Detection of Let-7 Micro RNA Using Polyaniline Fluorescence and Image Analysis Techniques
… immobilization and hybridization with mimic <em>let-7</em> is measured by fluorescence microscope and the image analyzed by MATLAB. A heuristic algorithm determines color threshold of the fluorescent active image. This image segmentation helps to determine the average pixel intensity representing …
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MicroRNAs: Tissue Expression and Role in 3T3-L1 Pre-Adipocyte Differentiation
… real time PCR. Several microRNAs, including let-7, were up-regulated at the late stage of 3T3-L1 adipogenesis. let-7 expression specifically increased during the late stage of 3T3-L1 differentiation and ectopic introduction of let-7 in 3T3-L1 cells before hormonal induction inhibited 3T3-L1 …
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MicroRNA cloning and bioinformatic analysis
… I. Two gene-regulatory noncoding RNAs (ncRNAs), let-7 RNA and lin-4 RNA, were previously discovered in the C. elegans genome. The let-7 gene is conserved across a wide range of genomes, suggesting that these ncRNAs represent a wider class of gene-regulatory RNAs. Both lin-4 and let-7 RNAs are …
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Microrna Regulation of Prostate Cancer Stem/Progenitor Cells and Prostate Cancer Development
… expressed, namely, four miRNAs (miR-34a, let-7b, miR-106a and miR-141) were under-expressed, and two miRNAs (miR-301 and miR-452) were over-expressed in the tumorigenic subsets compared to the corresponding marker-negative subpopulations. Among them, the expression patterns of miR-34, …
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Dicer loss induces an oncogenic epigenetic switch in mesenchymal stem cells
… the specification of the somatic state, we deleted Dicer in a murine mesenchymal stem cell model. Upon exploring the consequences of Dicer deletion, we identify a specific let-7 regulated mid-embryonic program within the global de-repression of miRNA targets accompanying Dicer loss. We further …
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Post-Transcriptional Regulation of Mammalian Gene Expression In Non-Coding Region of Target Rna
… <p>In addition, we used the established let-7-targeted high mobility group A2 (Hmga2) mRNA as a model system to study the mechanism of regulation of target mRNA by miRNAs in mammalian cells under physiological conditions. There have been no evidence of direct link between mRNA …
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The Expression of the Transcription Factor Broad and RNA-Binding Factors in the Midgut of the Mosquito Aedes Aegypti During Metamorphosis
… of <em>BR, BRAT,</em> and microRNAs <em>let-7</em>, and <em>miR-125</em>. Treatment with each hormone analog resulted in a change in <em>BR</em> expression.</p> <p>Finally, I evaluated the effect of nutrients on expression levels. Surprisingly, though most transcripts were down-regulated …
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Functional analysis of the microRNA genes of C. elegans
… loss-of-function mutations. We have generated deletion mutations in 87 miRNA genes in C. elegans, and performed an initial characterization of the 95 miRNA mutants available (86% of known C. elegans miRNAs). We found that the majority of miRNAs are not essential for the viability or development …
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A New Type of Programmed Cell Death in C. Elegans
… linker cell death is controlled by the microRNA let-7 and by the zinc finger transcription factor lin-29, both components of the main developmental timing pathway in the animal. Conducting a genome-wide RNAi screen, we have identified new candidate regulators of linker cell death. …
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Components of the Ubiquitin Proteasome System are Required for the Nonapoptotic Death of the C. Elegans Linker Cell
… may function in the same pathway as pqn-41. The let- 7 microRNA and its indirect target, the Zn-finger transcription factor LIN-29, also promote linker cell death, and may act early in the process. Our work suggests that components of the ubiquitin proteasome system (UPS) act to promote linker …
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The hybrid epithelial/mesenchymal phenotype and its implications in cancer metastasis
… regulating tumor-initiation potential (LIN28/let-7) predicts that a hybrid E/M, but not necessarily a fully mesenchymal, phenotype associates with higher tumor-initiation potential. Finally, integrating the core EMT network with intercellular Notch signaling, we predict that Notch-Jagged …
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Terminal uridyl transferases: TUT4/7-mediated RNA metabolism in cancer
… to be key regulators of the tumorigenic LIN28A/let-7 pathway. However, a full understanding of TUT4/7-mediated mechanisms of RNA control that impinge on tumorigenesis is still missing. In this thesis, I establish catalytic knockouts of TUT4/7 in two distinct cancer cell types to understand the …
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Modulation of Ago-miRNA regulatory networks by cis-sequence elements and target competition
… miRNAs other than the miR-290 family in ESCs and let-7 family in MSCs were expressed at concentrations below their total target pool. However, 8-12 miRNA families were expressed at near or greater than equimolar ratios with their pool of high affinity targets, and this affinity-partitioned …
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Functional study of miRNA-mRNA interactions in malaria mosquito An. gambiae
… of the indispensable blood feedings to complete their transmission cycles and to survive attacks from the mosquito's innate immune system. Normal egg development and mosquito immunity are tightly controlled by tissue- and stage-specific gene expression and coordinated by many signal …