Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"lentiviral transduction"”.
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Forward programming of human pluripotent stem cells to a megakaryocyte-erythrocyte bi-potent progenitor population: an in vitro system for the production of platelets and red blood cells for transfusion medicine.
… forward programming technique is a reliance of lentiviral transduction to overexpress the three TFs, which leads to a number of issues including heterogeneity and high experimental costs. To overcome this, I have developed an inducible iPSC line by inserting the forward programming TFs into a …
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Engineered Tracking and Delivery of Mesenchymal Stem Cells (MSCs)
… injury. First, MSCs were genetically modified by lentiviral transduction with a dual reporter gene vector containing monomeric red fluorescent protein and luciferase in order to track the cells <i>in vivo</i> in real time. It was found that polybrene, an additive commonly used during transduction, …
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Alpha-L-iduronidase transduced mesenchymal stem cells as a therapy for the treatment of CNS degeneration in mucopolysaccharidosis type I mice
… to over-express multiple MPS enzymes using a lentiviral system. MSCs were found to secrete up to 5,559 fold greater IDUA enzyme after lentiviral transduction in vitro, suggesting a greater potential to cross-correct MPS pathology than HSCs. Lentiviral transduction was stable and persistent in …
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Exploring the Functional Interaction Between CaMK-II and p53
… human cell line (HEK) was established through lentiviral transduction of p53 shRNA and verified with immunoblots and immunostaining assays. Next, the localization of CaMK-II and the cell growth rate in these cells was determined. In wild type HEK cells, catalytically inactive CaMK-II inhibited …
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A molecular genetic analysis of Crohn’s disease susceptibility loci in psoriasis
… CDKAL1 knock-down cell lines generated by sh-RNA lentiviral transduction were also analysed. This showed that CDKAL1 silencing results in reduced cell proliferation and altered cell cycle progression. Transcription profiling of the knock- down cells identified a number of differentially expressed …
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Suppressor of cytokine signalling 3 (SOCS3) turnover and regulation of human saphenous vein smooth muscle cell signalling and function
… inhibition of the vascular remodelling in NIH. Lentiviral transduction of WT and Lys-less SOCS3 in human saphenous vein (HSVSMCs) was highly efficient after 48 hours (>97%) and was sustained over 2 weeks. Lys-less SOCS3 was resistant to ubiquitylation contrary to WT-transduced HSVECs, and …
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Suppressor of cytokine signalling 3 (SOCS3) turnover and regulation of human saphenous vein smooth muscle cell signalling and function
… inhibition of the vascular remodelling in NIH. Lentiviral transduction of WT and Lys-less SOCS3 in human saphenous vein (HSVSMCs) was highly efficient after 48 hours (>97%) and was sustained over 2 weeks. Lys-less SOCS3 was resistant to ubiquitylation contrary to WT-transduced HSVECs, and …
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Identifying regulators of cytotoxic T cell function through molecular and genetic screening
… Additionally, stable Cas9 expression through lentiviral transduction was explored in primary CTL and related cell lines. This has the potential to allow selection of cells expressing the CRISPR machinery, providing a cleaner experimental system and the possibility of large-scale screening …
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The Impact of Clinically Relevant Culture on Human Haematopoietic Stem Cells: A Kinetic Analysis at Single Cell Resolution
… peripheral blood (mPB) culture during a lentiviral ex vivo gene therapy protocol. First, I characterise the molecular impact of a 62 hr lentiviral ex vivo gene therapy protocol on mPB LT-HSC, short-term HSC (ST-HSC) and CD34+ cells. I reveal that the ex vivo protocol dramatically rewires …
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Specification of Stem Cells and Niche During Hair Follicle Morphogenesis
… cells becoming WNT<sup>low</sup>. Using in utero lentiviral transduction and genetic mouse models, I created mosaic epidermis with gain- or loss-of-function for WNT signaling to demonstrate that juxtaposition of WNT<sup>low</sup> and WNT<sup>high</sup> cells is sufficient to confer SOX9+ cell fate …
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Biological role of conceptus derived factors during early pregnancy in ruminants
… deliver morpholino antisense oligonucleotides, lentiviral transduction of shRNAs, and CRISPR/Cas9 based genome editing were used to target the conceptus during elongation. Results of the studies established that: (1) IFNT is not only the maternal recognition of pregnancy signal, but is also …
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The role of hCLCA2 and hCLCA4 in suppression of breast cancer progression
… in parallel with several types of senescence. Lentiviral transduction of CLCA2 into MCF7 cells inhibited cell proliferation and cells showed senescence phenotype. To investigate the mechanism biochemically, we used pAd-Easy to express hCLCA2 in the model breast cancer cell line CA1d. A protein …
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Elucidating TRPA1 Ion Channel Aberrations in Oesophageal Adenocarcinoma.
… and wild-type TRPA1 are introduced to cells via lentiviral transduction. Missense mutations E179K, K186N, Q204K, K390N and H1018R reduce Ca2+ flow compared to WT, while L290P in the ARD, causes complete loss of Ca2+ entry following stimulation with Cinnamaldehyde, a TRPA1-specific agonist. OAC …
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Ion channels and electrical excitability in native murine anterior pituitary corticotrophs
… properties of native murine corticotrophs, a lentiviral transduction system was developed, using a minimal pro-opiomelanocortin (POMC) promoter to drive the expression of enhanced yellow fluorescent protein (eYFP), to allow highly efficient and specific labeling and identification of …
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Engineering of next-generation chimeric antigen receptors based on alternative binding domains
… single epitope. For this purpose, we synthesized lentiviral vectors containing the coding sequences of these molecules, to subsequently transduce T cell lines. Our research group has recently been working on the design of different CAR-like molecules for the treatment of various pathologies, …
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Characterization of a novel bioluminescent reporter protein for the non-invasive preclinical imaging of viral infection
… Human Alveolar Basal Epithelial) cells using a lentiviral transduction system to demonstrate how the reporter emits signal at different wavelengths [6]. Using a Bioluminescent Resonance Energy Transfer (BRET) system, the reporter exhibits both excitation and emission peaks, with the excitation …
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Heterogeneity in vascular smooth muscle cell gene expression and its association with clonal proliferation in models of vascular disease
… proliferation with growth factor treatment. Lentiviral transduction of the explanted VSMCs allowed for genetic manipulation, providing a platform to test the influence of individual genes on VSMC clonal expansion. In summary, this work identified a rare Sca1+ VSMC population which may …
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Genetic selection of cyclic peptide G-quadruplex ligands
… access. After porting the SICLOPPS system for lentiviral transduction, we carried out such a genetic selection in DT40 cells, assessing expression loss of a surface protein BU-1 produced by an endogenous G4-containing locus. Peptides identified that disrupt BU-1 expression were then validated …
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MENINGES AS AN OVERLOOKED TARGET FOR GLOBOID CELL LEUKODISTROPHY
… invasive site for in vivo gene therapy (GALC lentiviral transduction). Post natal day 1 (P.1) pup mice have been injected in meninges with lentiviral vector (LV) overexpressing GALC gene and then analyzed at P.30 when the GLD is severe. Of note, GALC transduction in meninges at P.1 induced at …
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Characterisation of Kaposi's sarcoma-associated herpesvirus (KSHV)-driven pathology and disease outcome in HIV infected South African patients
… type and mutant EPHA2 open reading frames by lentiviral transduction. These engineered cells were assessed for baseline EPHA2 phosphorylation levels and susceptibility to KSHV infection utilising recombinant KSHV in binding, internalisation and infection assays. We found that the EPHA2 mutant …
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