Global ETD Search
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Showing 1 to 19 of 19 for “"leader sequence"”.
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Investigations of the Mechanism and Substrate Scope of the Enzymes Involved in Lantibiotic Biosynthesis
… Mutations of highly conserved residues in the leader sequence as well as deletions of the N-terminal residues in the leader sequence of LctA did not affect dehydration by LctM.
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Investigation of the Enzymes Involved in Lantibiotic Biosynthesis: Lacticin 481 and Haloduracin
Finally, removal of the leader peptide from the modified precursor peptides is the final step in the maturation of lantibiotics, and is required for achievement of full biological activity. In lacticin 481 maturation, this peptide cleavage reaction is performed by the N-terminal protease domain of …
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The Regulation of NAP4 in Saccharomyces cerevisiae
… upstream open reading frames (uORFs) in the 5' leader sequence of HAP4 mRNA; (2) proteasome-dependent degradation of Hap4; and (3) identification of two negative regulators of HAP4 expression, CYC8 and SIN4. I also report differential patterns of Hap4 cellular localization which depends on (1) …
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Isolation of the aldose reductase gene (XvAld1) from the resurrection plant Xerophyta viscosa, and characterisation of the gene product and transgenic plants expressing the gene
… The observed nucleotide differences between the sequenced clones could reflect polymorphisms between different copies of the gene. An 870-bp clone of the 5′ untranslated region, matching the 5′ leader sequence on the XvAld1 cDNA was analysed for cis-acting response elements. Many of the sequence …
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Studying sequence effects of mRNA 5' cap juxtapositions on translation initiation rate using randomization strategy of the extreme 5' end of mRNA
… but also the properties of mRNA 5’transcript leaders (5’TL). In most cases of cap-dependent translation, translation initiation begins with the formation of the preinitiation complex (PIC) loading and accommodation onto the m7G capped 5’end of mRNA, facilitated by m7G cap – eIF4F interactions. …
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동물 세포의 세포질에서 발현된 IgG 의 중쇄와 경쇄간의 조합 여부 및 항원결합 활성 분석
… 중쇄와 경쇄간의 결합 및 항원 결합능력을 조사하였다. 세포질에서 발현되도록 선도 서열 (leader sequence)을 제거하여 중쇄와 경쇄를 동시에 발현하는 벡터를 제조하였다. ch 3D8 IgG1의 기본구조 외에도 중쇄의 경첩 부위 (hinge region) 및 중쇄-경쇄 사이의 cys 잔기를 세린 (Serine, Ser) 잔기로 치환한 3D8 IgG1 변이체들을 HEK293 세포질에 발현시켰다. ch 3D8 IgG1 항체를 발현시켜 얻은 세포 파쇄액을 이용하여 면역효능흡착법 (ELISA)과 면역침강법 …
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From Peptide Precursors to Lanthionine -Containing Peptide Antibiotics Using the First Isolated and Active Lacticin 481 Synthetase: Mechanistic Studies and Peptide Engineering
… the modified peptide in the absence of the leader sequence and the first Lys residue showed biological activity. Mechanistic studies with the active LctM disclosed that the activity of LctM absolutely requires both Mg2+ and ATP. The products of ATP were determined to be ADP and phosphate. …
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Cloning, High-Level Expression, and Characterization of Escherichia Coli Cytochrome B(562)
… length gene, including the promotor. The DNA sequence analysis of the gene revealed the presence of a leader sequence, with the characteristics of an E. coli signal sequence, in front of the coding sequences for the mature protein. Localization studies on the overexpressed protein revealed …
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Mechanistic Investigations of Cyclase Enzymes Involved in Lantibiotic Biosynthesis
… and dehydrobutyrine residues in the peptide. Sequence alignments of both the LanC and LanM families of enzymes reveal four conserved residues, two cysteines and two histidines. Mutants of SpaC replacing the conserved cysteines with alanines were generated and characterization by both EXAFS and …
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Multidrug resistance proteins (MRP) transport the cytokine/nuclear protein High Mobility Group Box 1 (HMGB1) across membranes
… stimulus is received. HMGB1 does not possess a leader sequence, a sequence usually present at the Nterminus of secreted proteins that is sufficient to address them through the ER/Golgi exocytic pathway. This work focused on the molecular mechanism by which HMGB1, as a leaderless protein, can be …
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Regulatory intricacies that confer temporal control of conjugation and antibiotic production functions in Streptomyces: new variations on old themes
… the fact that ipoA contains a TTA codon in its leader sequence, makes ipomicin a strong candidate for bldA regulation.
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Studies of Non-Classical Antigen Presentation Molecules: Functional Analysis of Mouse Qa-1 and Characterization of the Porcine CD1 Gene Family
… peptide (Ins II; ALWMRFLPL) in the insulin leader sequence indicates that Qa-1 expression by pancreatic $\beta$-cells may play a role in the progression of islet inflammation. Further, the lack of Qa-1$\rm\sp{a}$ on NIT-1 cells, pancreatic $\beta$-cells derived from NOD mice, forces …
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Adenovirus-based exogenous gene expression in mammalian cells
… the major late promoter (MLP) and the tripartite leader sequence (TPL). The green florescence protein (GFP) was used as a reporter gene. Various aspects of gene expression were examined including DNA delivery and stability inside the cells as well as mRNA transcription and protein expression. …
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DEVELOPMENT OF SMALL MOLECULES AS INHIBITORS OF FTSZ AND RNPA ACTING AS POTENTIAL ANTIMICROBIAL AGENTS
… complex that promotesthe removal of the 5’ leader sequence from precursor tRNA, inducing tRNA maturation. Shortly after the discovery of the essentiality of RnpA, the investigation of RnpA inhibitors as potential anti-staphylococcal compounds started. Within the second main body of this …
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Proteomic Analyses of HIV-1 Infected T-cells and the Functional Characterization of Cyclophilin B During HIV-1 Infection
… effect. The N-terminus of CypB contains an ER leader sequence necessary for secretion as well as a nuclear localization signal. Passive transfer experiments showed that secreted CypB did not impact HIV infection. However the deletion of the N-terminus resulted in a pronounced mislocalization of …
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Identification and characterisation of an extrachromosomal element from a multidrug-resistant isolate of Trypanosoma brucei brucei
… different approaches sere attempted before a sequence which hybridised to the 6.6 kbp element its eventually isolated. This sequence is represented by a 108 bp repeat sequence which forms long arrays of tandem repeats. Since N/a III is the sole restriction enzyme that cuts within the repeat, …
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Functional Organization of Molecular Memories in the CRISPR-Cas Immune System
… direct repeats intercalated by variable spacer sequences (~30bp) of foreign origin. Upon infection, spacers are excised from the foreign genome and integrated into the array. The array is then transcribed and parsed into individual CRISPR RNAs, each containing a single spacer sequence, which are …
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Paleovirological Analyses of Endogenous Retroviruses and Host Innate Immune Effectors
… mouse genomes, respectively, are comprised of sequences of retroviral origin. Occasional infection of germ line can lead to integrated retroviral genomes being vertically inherited as host alleles. During thousands to millions of years, some of these sequences acquired inactivating mutations …
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Generation and characterization of HIV-1 subtype C candidate vaccines that will induce high titre antibody responses to HIV-1 envelope glycoprotein
… platform regimen was developed using antigen sequences from the predominant circulating HIV-1 subtype (subtype C) in South Africa. Specifically, this involved use of the envelope glycoprotein sequence of the CAP256 superinfecting virus (CAP256_SU) from the CAPRISA 002 cohort, and a mosaic Gag …