Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"leader peptide"”.
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Structural investigation of lanthipeptides involved in substrate controlled pathways and methods for leader peptide removal
… synthesized and post-translationally modified peptides are a family of natural products with diverse activities, with lanthipeptides as one of the most prominent classes. The diverse ring topology of lanthipeptides is formed by four classes of enzymes with substrate tolerance that holds …
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Investigations of the Substrate Specificity of Lacticin 481 Synthetase and Utilization in Peptide Engineering Applications
The promiscuous activity of LctM toward LctA prepeptides containing nonproteinogenic amino acids prompted the evaluation of LctM as a general catalyst for the introduction of post-translational modifications in non-lantibiotic peptides fused to the LctA leader peptide. LctM was utilized to …
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Mechanistic studies of class II lanthipeptide synthetases and yeast surface display of lanthipeptide leader peptides
Lanthipeptides are natural products that belong to the family of ribosomally synthesized and posttranslationally modified peptides (RiPPs). They contain the characteristic lanthionine (Lan) or methyllanthionine (MeLan) structures that contribute to their diverse biological activities. ProcM is a …
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New chemical and biosynthetic methodologies for the study of lanthipeptides
… revealed that a common biosynthetic route to peptide natural products involves ribosomally synthesized and posttranslationally modified peptides (RiPPs). One of the largest classes of RiPPs is the lanthionine-containing peptides (lanthipeptides), which are characterized by intramolecular …
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Engineering of the microviridin post-translational modification enzymes for the production of synthetic protease inhibitors
… synthesized and post-translationally modified peptides (RiPPs) are a group of natural products generated by the action of post-translationally modifying enzymes on precursor peptides translated from mRNA by ribosomes. The great substrate promiscuity exhibited by many of the enzymes from RiPP …
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Biosynthetic engineering of plantazolicin natural products
… synthesized and post-translationally modified peptide (RiPP) natural product that exhibits extraordinarily narrow-spectrum antibacterial activity towards the causative agent of anthrax, Bacillus anthracis. During PZN biosynthesis, a cyclodehydratase catalyzes cyclization of cysteine, serine, …
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Activities of Bacteroides Conjugative Transposons: CTnGERM1 and CTnDOT
… of regulated production of GUS. A 3 amino acid leader peptide which extends into this hairpin was found to be essential for Tc-induction. Abolishing the translation of this 3 as leader peptide by putting in a stop codon, or changing any amino acid without changing the hairpin structure …
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Expanding the structural diversity of the RiPP class of natural products
… synthesized and post-translationally modified peptides (RiPPs) are a diverse and rapidly expanding class of natural products. Despite starting out as linear chains of amino acids, RiPP natural products acquire structural diversity ranging from small molecules like microcin C7 to 48-mer …
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An analysis of the effect of mutations in the leader region of the threonine operon of Escherichia coli on attenuation or transcription termination
… An open translational reading frame in the leader region preceding the structural genes contains 21 codons with eight threonine and four isoleucine codons. The model for attenuation proposes that regulation depends on the translation of the 21 amino acid leader peptide. …
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Investigation of the Lacticin 481 Synthetase and Its Substrate LctA in the Production of the Antibiotic Lacticin 481
… synthesized and post-translationally modified peptide antimicrobial agents produced by Gram-positive bacteria that contain thioether cross-links termed lanthionines. Lantibiotic biosynthesis begins with a precursor peptide (LanA) consisting of leader and propeptide regions. The …
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Investigation of the Enzymes Involved in Lantibiotic Biosynthesis: Lacticin 481 and Haloduracin
Finally, removal of the leader peptide from the modified precursor peptides is the final step in the maturation of lantibiotics, and is required for achievement of full biological activity. In lacticin 481 maturation, this peptide cleavage reaction is performed by the N-terminal protease domain of …
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Investigation and application of substrate tolerant thioether forming enzymes
… thioether containing natural products. Lanthipeptides contain thioether rings and have demonstrated diverse biological activities. This group of natural products are of great interest due to their diverse ring topology and high substrate tolerance of the biosynthetic enzymes. Amongst them …
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Biosynthesis of the antimicrobial peptide epilancin 15X
Lantibiotics are polycyclic antimicrobial peptides that are ribosomally synthesized and posttranslationally modified to their biologically active forms. The recently discovered lantibiotic epilancin 15X produced by Staphylococcus epidermidis 15X154 is active against several pathogenic bacteria, …
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Characterization of the MATα pre-/pro- peptide by mutagenesis as a means to optimize secretion in pichia pistoris
… <em>MAT</em>α <em>pre-pro</em> signal leader (<em>MAT</em>α) has led to increased protein secretion in most cases. <em>MAT</em>α is thus used in the production of 90% of all proteins secreted in <em>P. pastoris</em>. However secretion efficiency still remains a problem.</p> <p>It has …
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Engineering Secreted Proteins for Gene Transfer and DNA Vaccination
… by fusing the human interleukin-2 signal peptide to <em>Renilla</em> luciferase. We further modified the <em>Renilla</em> luciferase gene using site-directed-mutagenesis to obtain a mutant form of<em> Renilla</em> luciferase, SRUC3, with dramatically improved stability. SRUC3 provides a …
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Mechanistic studies of lanthipeptide biosynthesis
… novel natural products are demanded. Lanthipeptides are ribosomal peptides with post-translationally incorporated thioether crosslinks named lanthionine. This family of natural products has garnered substantial attention during the past few decades due to their favorable biological …
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Investigating the role of HLA class I derived peptide in alloreactivity.
The peptide complexed with human leukocyte antigen (HLA) can be said to have a significant role in the generation of alloresponses, both in terms of cellular and humoral alloresponses. Previous researchers have identified minor histocompatibility antigen (mHAG) derived peptides such as HA-1, and …
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Discovery and characterization of ribosomally synthesized and post-translationally modified peptide natural products
… synthesized and post-translationally modified peptide (RiPP) natural products are genetically encoded peptide-derived secondary metabolites whose structures can be predicted based on gene sequence and identity of tailoring enzymes within the biosynthetic gene cluster. Post-translational …
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Characterizing peptide-modifying enzyme activity in RiPP biosynthesis
… synthesized and post-translationally modified peptides (RiPPs) use enzymatic chemistry to diversify simple peptide backbones into architecturally complex and functionally rich natural products. These dedicated peptide-modifying enzymes install heterocycles, macrocycles, acyl groups, halogens, …
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MUTACIN IV PRODUCTION IN STREPTOCOCCUS MUTANS UA159: CHARACTERIZATION, REGULATION, AND MECHANISM OF SELF-IMMUNITY
… by ribosomally synthesized small cationic peptides called mutacins (bacteriocins) secreted by S. mutans. Although for long time mutacins are regarded as important virulence factors, it is not clear how these peptides work, what is the mechanism of self-immunity, and how they are regulated. …
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