Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"lck"”.
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Die transkriptionelle Regulation der Proteintyrosinkinase p 56 lck als Schlüsselenzym der Thymozytenreifung
… der lymphozytenspezifischen Proteintyrosinkinase lck untersucht. Das Hauptaugenmerk richtete sich auf die Beteiligung der Familie der NF-AT-Transkriptionsfaktoren an der Kontrolle der Promotoraktivität. Es konnte zunächst gezeigt werden, dass NF-ATs aus Zellkulturzellen sowie aus frisch isolierten …
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Strukturelle Untersuchungen der Lck-Domänen unique und SH3 und ihre Wechselwirkungen mit zellulären und viralen Proteinen
… Spezifischen Kinase (unique- und SH3-Domäne, LckU3) mittels NMR-Spektroskopie in Lösung strukturell zu charakterisieren. Auf Basis dieser NMR-Untersuchungen war es möglich, die Wechselwirkungen von LckU3 mit verschiedenen Bindungspartnern auf atomarer Ebene zu verfolgen. Darüber hinaus wurden …
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Functional relationships among lck kinase and the lat and grb2 adaptors in the intracellular signaling cascade of the tcr/cd3 complex: implications in thymic development and lymphocyte activation
… that happens to be doubly deficient in LAT and Lck. These cells require simultaneous expression of LAT and Lck to recover intracellular signals upon stimulation of the TCR/CD3 complex. In the second chapter we delve into the role of the LAT adaptor in TCR signaling. It has been shown in vitro, …
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Spatial Dynamics and the Mechanoresponse in CD4+ T Cell Activation
… human T cells is developed. The Src kinase Lck, though predominantly cytosolic, is an ideal candidate for the coupling of the TCR and CD28 pathways. Membrane associations bring Lck in contact with these receptors, where mediation of its active state by kinase activity and regulation of its …
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Developing Targeted Therapies for T-cell Acute Lymphoblastic Leukemia
… & pharmacodynamic (PK & PD) modeling; and 5) an LCK degradation approach can achieve better therapeutic efficacy than dasatinib in T-ALL. <strong>Methods</strong> We applied a network-based Bayesian Inference of Drivers (NetBID) algorithm using RNA-seq data to infer activity of each gene and …
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Regulation of T-cell signaling networks
… of the T-cell receptor (TCR) by its kinase Lck and a negative regulator of Lck called Csk. We show that the mechanism by which the cell regulates TCR levels, together with the manner in which Lck activates the TCR produces interesting behavior, such as a "perfectly adaptive" system and a …
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Virtual Screening of Multi-Target Agents by Combinatorial Machine Learning Methods
… inhibitors pairs (EGFR-Src, EGFR-FGFR, VEGFR-Lck, Src-Lck), I applied the COMBI-SVMs to the identification of dual-target antidepressant agents of 7 target combinations (serotonin transporter paired with noradrenaline transporter, H3 receptor, 5-HT1A receptor, 5-HT1B receptor, 5-HT2C receptor, …
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Methods to Improve Virtual Screening of Potential Drug Leads for Specific Pharmacodynamic and Toxicological Properties
… protein tyrosine kinase inhibitors (Lck) and phosphoinositide 3-kinase inhibitors (PI3K). Further investigated were toxicological data sets such as eye and skin irritation, compounds that produce reactive metabolites, and hepatotoxicity. To the best of our knowledge, the Lck and PI3K …
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Protein segregation and conformation in antigen-receptor triggering: a quantitative fluorescence microscopy study
… surface. A short section focusing on the kinase Lck is also included. The feasibility of using Förster resonance energy transfer to identify spatiotemporal changes in Lck conformation is explored. Understanding antigen-receptor triggering is crucial, as failure to discriminate between self and …
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Develoment and Application of Chemical Strategies to Study Protein Fatty-Acylation in Eukaryotes
… protein turnover. Dual pulse-chase analysis of Lck, a tyrosine kinase required for T-cell signaling, revealed accelerated palmitate cycling upon T-cell activation. Pharmacological perturbation of Lck palmitate turnover suggests yet uncharacterized serine hydrolases contribute to dynamic …
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PKB/Akt : a critical regulator of lymphocyte, development and function
… myr PKB enhances activation of the kinases Lck, Raf and Erk after TCR/CD3 stimulation. Thus, myr PKB renders proliferative responses of thymocytes more sensitive to TCR signals by positive regulation of the Lck-Raf-MEK-Erk signalling pathway. Studies on the cellular location of the tg …
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Total synthesis of ulocladol A and analogues alternariol 0-methyl ether and alternariol
… kinase inhibitory activity against the enzyme Lck. It was isolated from the marine sponge Callyspongia vaginalis in 1999. Prior to the commencement of this project there had been three reported syntheses of ulocladol A, all of which started from intermediates prepared in the synthesis of …
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A Preliminary Investigation of Physical-Chemical Treatment of Slagging Gasifier Aqueous Effluent
… oxidation seems to be more economical.</p> <p>LCK activated carbon, manufactured by Union Carbide, proved to be the preferred granular carbon for slagging gasifier liquor treatment.</p> <p>A total capital investment of approximately $14,748,000 can be expected for a facility capable of …
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The interferon alpha receptor utilises T-cell receptor-associated proteins for signalling
… proteins for this pathway to occur such as CD45, Lck and Zap70. In this study evidence is presented to show that two further TCR-associated proteins are phosphorylated in response to the IFNAR; the 95kDa guanosine nucleotide exchange factor, Vav, and the 76kDa adaptor protein Slp76. This proceeds …
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TRP channels and a dextran sulfate activated rise in intracellular Ca2+ and Na+ in a subpopulation of double positive thymocytes
… activation of the Src tyrosine kinase Lck also appears essential as PP2 abolished the rises. Downstream of Lck, both PI3K and PLC activation is necessary as their inhibition by LY294002 and edelfosine, respectively, abolished the rises. The rises do not involve store release via IP3 …
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TRP channels and a dextran sulfate activated rise in intracellular Ca2+ and Na+ in a subpopulation of double positive thymocytes
… activation of the Src tyrosine kinase Lck also appears essential as PP2 abolished the rises. Downstream of Lck, both PI3K and PLC activation is necessary as their inhibition by LY294002 and edelfosine, respectively, abolished the rises. The rises do not involve store release via IP3 …
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Differentiation into Effector Cells with Distinct Memory Fates: Contributions of Viral Dose and Functional Avidity
… was correlated with decreased ZAP-70 and Lck expression as well as their phosphorylated products, both important molecules in T cell receptor membrane proximal signaling. These results highlight the potential contribution of avidity in the differentiation and evolution of the T cell …
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The role of the adapter protein TSAd in T cell activation
… cells. TSAd interacts with the tyrosine kinases Lck and Itk and modulates their activity. These kinases are critical for the activation of T cells, however, the consequences of TSAd´s modulation of their activities are poorly understood. The aims of this study are: 1. Establish method for NF-κB …
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The Dual Role of OCA-B in B Cell Development and Signaling
… the identified OCA-B target genes (e.g. Kcnn4, Lck, CyclinD3, and Cdc37) play important roles in cell signaling; however, their specific functions in B cell development and activation are largely unknown. From multiple primary B cell stages, we have found that key OCA-B target genes are …
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Costimulatory function of CD44
… and src-family kinases, two of which, fyn and lck, were found to be associated with CD44. By cross-linkage of CD44 these kinases were redistributed into so called lipid rafts. It is supposed that for T cell activation a relocation of the TCR/CD3 complex into the same membrane microdomains is …
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