Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 77 for “"lateral flow"”.
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Increased sensitivity for lateral flow immunoassays through signal amplification methods
… and inexpensive sandwich-immunoassay-based lateral flow tests (LFTs, also known as lateral flow immunoassays, LFIAs, and rapid diagnostic tests, RDTs) are one of the general approaches for detection of disease targets (e.g. nucleoprotein of the influenza virus). However, the use of these …
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Development of Rapid Lateral-flow Immunochromatographic Test for the Diagnosis of Chikungunya Virus Infection
… The purpose of this study was to establish a lateral-flow immunochromatographic test (LF-ICT) ideal for applications as rapid point-of-care test for the diagnosis of acute CHIK virus infection. METHODS: Three CHIK virus structural recombinant proteins, capsid protein and, E1 and E2 envelope …
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Development of gold nanoparticles based lateral flow assay for detection of food and water-borne pathogens
… The use of gold nanoparticles (AuNPs)-based lateral flow assay (LFA) for the detection of these pathogens is gaining traction among researchers as an alternative to conventional methods for on-site testing in places where there is no proper infrastructure, particularly low-resourced areas. …
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Preparation and evaluation of polymer microspheres for enhanced lateral flow immunoassay: the case study for malaria
… be developed as viable detection reagents for lateral flow technology. This body of work outlines the development of this new high capacity polymer microsphere technology for suitability to flow on lateral flow membranes, and highly specific biomarker detection for immunoassay development. …
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Ground-penetrating radar images of a dye tracer test within the unsaturated zone at the Susquehanna-Shale Hills CZO
… radar (GPR) were used to image preferential flow paths in the shallow, unsaturated zone on hillslopes in two adjacent watersheds within the Susquehanna-Shale Hills Critical Zone Observatory (CZO). At each site we injected about 50 L of water mixed with brilliant blue dye (4 g/L) into a trench …
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Discovery and development of diagnostic biomarkers for human African trypanosomiasis
… for the test.</p> <p>Our aim was to develop a lateral flow test based on trypanosome antigens. We used sera from <em>T. b. gambiense</em> infected and non-infected patients to identify infection specific diagnostic trypanosome proteins. The trypanosome proteins identified were then cloned into …
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Development of a plant-made immunoassay for the detection of Porcine circovirus infections in South African swine herds
… aim of this study was to develop an inexpensive lateral flow device to be able to test for PCV infection in pig herds. Production of PCV-2 CP in Nicotiana benthamiana via transient Agrobacterium-mediated expression was optimised by comparing two expression vectors, pEAQ-HT and pCBP2, and VLPs …
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Point-of-care immunoassay system using carbon nanotube labels
… direct electrical measurement was implemented on lateral flow system and microfluidic integrated interdigitated array microelectrodes. These sensing mechanisms in simple and miniaturized system provided higher sensitivity and autonomous flow control for rapid detection aimed at point-of-care …
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Immunochromatography assays to diagnose tropical viral pathogens using gold nanoparticles
… lmmunochromatography assays use capillary flow and the accumulation of ligand-coated nanoparticles to detect the presence of target proteins. We build multiplexed diagnostics that allow the detection of the four serotypes of Dengue and Zika, and validate the performance of these diagnostics …
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Surface-Enhanced Raman Spectroscopy Enabled Microbial Sensing
… We first developed a labeled SERS-based lateral flow test for virus quantification. Optimization of the lateral flow design and digital signal analysis enabled high sensitivity towards SARS-CoV-2. To elicit a comprehensive understanding of pathogen infection, label-free living-cell SERS …
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Development of a rapid molecular method for detection of C. perfringens and other pathogens in water sources
… with the detection of generated amplicons on lateral flow devices, excludes the need for detection methods requiring electrified equipment. The study described here aimed to evaluate RPA, combined with lateral flow detection of labelled amplicons, as a tool to detect deoxyribonucleic acid …
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Towards paper-based micro bio-sensing of biomarker anti-mycolic acid antibodies for TB diagnosis
… aimed to contribute towards the development of a lateral flow immunoassay termed MALIA (Mycolate Antibodies Lateral flow Immuno Assay). This diagnostic has the unique lipid antigen MA as the immobilised capture agent and custom developed monoclonal anti-MA chicken antibodies (gallibodies) as the …
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Evaluation of future meteorological and hydrological drought in the Rufiji basin
… Index (SPEI), and the Streamflow Drought Index (SDI)—to analyze 12-month drought events within the basin. This approach allows for a examination of drought characteristics, including their frequency, intensity, as well as its spatial distribution by the use of hydrological …
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Development of Au-Pt Nanoflowers as Nanoenzyme for Highly Sensitive Immunoassay: Application to the Detection of Melioidosis Biomarker
… study, we present a core shell gold-platinum nanoflowers (Au-Pt NFs) as signal labels in immunoassays for sensitive detection of capsular polysaccharides (CPS) expressed on the cell envelopes of Burkholderia pseudomallei causing Melioidosis, an infectious disease of global health concern. …
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Variable Viscosity Thermal Convection at Infinite Prandtl Number in a Thick Spherical Shell
… dominant rheological influence controlling the flow characteristics--lateral variation of viscosity plays a secondary role. Both a high viscosity lid and a viscosity increase below 660 km depth provide a general shift to lower harmonic degrees in both thermal anomalies and kinetic energy. With …
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Rational hydrogel design for point-of-care bioassays
… technologies are largely centered on lateral flow assays. Hydrogel microparticles have been used extensively for bioassays due to their nonfouling nature and ability to be functionalized with different types of biomolecules. Here, we use polyethylene glycol hydrogel particles to …
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Aptamers that bind SARS-CoV-2 spike protein for COVID-19 rapid diagnostics and therapeutics
… to the S N-terminal domain. We applied SNAP1 in lateral flow assays (LFAs) and ELISAs to detect UV-inactivated SARS-CoV-2 at concentrations as low as 5 x 105 copies/mL. In chapter 2, we describe a new aptamer that also binds wild-type spike protein at the NTD, named SARS-CoV-2 spike protein …
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Design, Development, and Validation of an End-User Photo-Thermal Sensing Platform for Rapid Detection and Quantification of Analytes in Fluidic Samples
… results. Portable and inexpensive tests exist – Lateral Flow Immunoassays – however these tests are only qualitative and frequently have an inferior limit of detection. To date, several sensing devices have been designed to interrogate these LFIAs and decrease their limit of detection, however, …
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Drought on the North American high plains: modeling effects of vegetation, temperature and rainfall perturbations on regional hydrology
… between them. The model was constructed using ParFlow-CLM, which represents surface and subsurface processes in detail with physically based equations. Numerical experiments were run to perturb vegetation, precipitation and temperature separately, as well as a baseline scenario with no changes and …
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