Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"late gene"”.
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Characterization of the Human Cytomegalovirus UL75 Late Gene Promoter
<p>Gene expression during productive infection by the human cytomegalovirus (HCMV) occurs in an ordered and sequential manner, beginning with immediate early (IE), then early (E) and finally late (L) gene expression. Significant work has addressed the regulation of IE and E gene expression while …
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Regulation of late and very late gene expression in both lytic and persistent baculovirus infections
… pathogen, with a biphasic replication cycle generating both budded viruses (BVs) and occlusion bodies (OBs). While BVs are released from the cell membrane mainly in the late phase of infection, OBs are produced in the subsequent very late phase and released after cell lysis. A characteristic …
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ORGANIZATION AND FUNCTION OF SIMIAN VIRUS 40 ORIGIN REGION SEQUENCES IN LATE GENE TRANSCRIPTION AND REPLICATION.
… OF SIMIAN VIRUS 40 ORIGIN REGION SEQUENCES IN LATE GENE TRANSCRIPTION AND REPLICATION.
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The dual role of lef-2 in DNA replication and late gene expression during baculovirus-replication
… DNA replication and is involved in expression of late and very late gene promoters. In earlier work, the isolation of a virus mutant (VLD1) deficient only in very late protein synthesis was attributed to a point mutation in lef-2. DNA replication and late protein production remained largely …
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Regulation of Human Papillomavirus Type 16 Late L1 mRNA Splicing
… HPV-16 infects epithelial cells and HPV-16 gene expression is tightly linked to the differentiation stage of the infected cells. Early HPV-16 genes are expressed in basal layers of the epithelium whereas the late genes which encode highly immunogenic viral structural proteins are only …
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The Intracellular Kinetics of HIV-1 Replication
… life cycle and to determine how they are correlated with one another. Using a variety of techniques including quantitative microscopy we tracked the timing of these events, both in bulk infected cultures and in single infected cells, and generated a time line of the HIV-1 replication cycle. We …
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Structural Studies of Three Factors That Affect the Prokaryotic Transcription Cycle; Microcin J25, LAMBDA Q and T4 GP33
… RNAP to readthrough termination signals in phage late genes; and Gp33, a T4 phage protein that is a co-activator of T4 phage late gene transcription. We determined that the Microcin J25 peptide (MccJ25) has a lassoed-tail structure using mass spectrometry and NMR. The 21 amino acid MccJ25 peptide …
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Murine Cytomegalovirus Encodes Proteins that Regulate Viral Late Transcription
… to virion structure, genome organization, gene expression, tissue tropism, and clinical manifestations. In my graduate studies, I discovered the function of two novel MCMV proteins during in vitro infection. I demonstrated that ORF M79 encoded protein pM79, and ORF M92 encoded protein pM92. …
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Functional Characterization of the Streptococcus sanguinis com Regulon
… of the cardiac valves. S. sanguinis possesses genetic competence, the ability to acquire exogenous DNA into its genome. In the well characterized system of S. pneumoniae, genetic competence requires a quorum sensing system encoded by the early comCDE genes, as well as late genes controlled by …
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Repurposing the human immunodeficiency virus (HIV) integrase inhibitor raltegravir for the treatment of felid alphaherpesvirus 1 (FHV-1) ocular infection
… inhibits both viral DNA synthesis initiation and late gene expression, a mechanism consistent with inhibition of viral ICP8. Finally, RNA sequencing was used to explore the indirect effects of raltegravir on the host. It was found that raltegravir treatment promoted the expression of …
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Examination of the role of early viral protein expression in MVA-induced NF-κB activation
… of various host range and immunosuppressive genes in this strain. The work presented here focused on investigating the viral molecular mechanism responsible for the activation of NF-κB. To that end, we found that infection with MVA in the presence of AraC, a drug that inhibits viral DNA …
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Natural Killer Cell Responses to Human Cytomegalovirus
… of the beta-herpesvirus family, establishes latent infection in the face of robust humoral and cell-mediated immune responses. In spite of significant efforts, the mechanisms implicated in protective immunity during HCMV infection are still not fully understood. To evade the potent and …
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The Role of Viral Protein pUL21a in Human Cytomegalovirus Infection
… of many of the roughly 200 putative HCMV genes are still unknown. One of these genes, UL21a, was shown to be critical for efficient viral replication in two large-scale mutagenesis studies. UL21a encodes for a putative protein that is 123 amino acids and has no homology to any known …
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Inactivation mechanisms of adenovirus from exposure to polychromatic ultraviolet light irradiation
… greater than 99.99%. In contrast, early and late gene transcription, and viral DNA replication were inhibited to different extents by UV irradiation at all three peak emissions, which suggested that different mechanisms might be dominating such disruptions for each wavelength range. The …
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The CD4⁺ T Cell Response to Human Cytomegalovirus
… the virus then establishes a lifelong latent infection such that the host is unable to eradicate the virus completely. This then results in periods during which the virus reactivates and can replicate. In an immunocompetent host, this replication is eventually controlled by a robust …
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Study of the roles of Cyprinid herpesvirus 3 ORF134 in the biology of the infection
… subspecies are economically important. In the late 1990s, a highly contagious and lethal pathogen called koi herpesvirus (KHV) or cyprinid herpesvirus 3 (CyHV-3) began to cause severe financial losses in these two carp industries worldwide. In 2005, CyHV-3 has been classified in the …