Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 52 for “"late endosomes"”.
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Axonal Translation and Links to Neuropathies
… local translation can occur on Rab7a-associated late endosomes, where mRNA recruitment and translation are coordinately regulated. Notably, mRNAs encoding mitochondria-related proteins are found translating on late endosomes docking in the vicinity of mitochondria, suggesting late endosomes act …
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"Molecular Analysis of Virulence Factors Unique to Leptopilina heterotoma-a Parasitic Wasp of Drosophila spp."
… with compartmental marker signals of the late endosomes, marked by Rab7-GFP; higher VLP signals correlated with late endosomes in plasmatocytes for animals that underwent a longer post-infection recovery time. Some colocalization of VLP was also observed with the Rab5-GFP as well.</p>
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Investigating the Biological Functions of the Protein Kinase WNK1 in the Regulation of Cytoskeletal Structures and Membrane Trafficking
… this perinuclear distribution and how it might relate to the biological functions of WNK1 from two aspects. First, I investigated cytoskeletal structures mainly focused on the microtubules. WNK1 localized on mitotic spindles during mitosis as well as interphase microtubules. Depletion of WNK1 …
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Somatostatin Receptor 2 Regulation By C-Terminal Motifs and Pdz Domain Proteins
… occludens-1 (PDZ) domain interactions often regulate the trafficking and signaling potential of other GPCRs, we examined the role of the sst2 PDZ ligand and additional C-terminal residues in controlling its intracellular trafficking, signaling, and regulation. We determined that sst2 traffics …
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SYNTHESIS, CHARACTERIZATION, IN VITRO EVALUTION, AND PRECLINICAL PROFILING OF β-CYCLODEXTRIN POLYROTAXANE FAMILIES FOR USE AS POTENTIAL NIEMANN-PICK TYPE C THERAPEUTICS
… cholesterol (UC) trafficking through the late endosomes/lysosomes (LE/LY) of every cell of the body. Disruption of this pathway leads to abnormal accumulation and storage of UC and other lipids. A broad range of visceral and neurological symptoms result from this accumulation exhibiting a …
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The Ubiquitin Ligase Ube4B Is Required For Efficient Epidermal Growth Factor Receptor Degradation
… proteins reside on the plasma membrane is regulated by endocytosis, a process that can inactivate these proteins by removing them from the membrane and may ultimately result in their degradation. Proteins are internalized and pass through multiple distinct intracellular compartments where …
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Kinetic analysis of endosome processing : maturation of early endosomes and vesicular traffic to lysosomes
… of the endocytic contents markers within early endosomes that mature to form non-fusogenic late endosomes (maturation model). This maturation phase is followed by a segregation phase where intermingling of contents between vesicles no longer takes place. To establish the mechanism(s) involved in …
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Optimization of organelle fractionation methods for quantitative analysis of gene delivery trafficking kinetics
… hepatocyte lysate into nuclear, early endosomal, late endosomal/lysosomal, and cytoplasmic fractions. We found that according to the Horseradish Peroxidase assay, late endosomes and lysosomes consistently localize to fractions 11-13 and early endosomes in fractions 18 to 21. There was minimal …
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The Dynamics and Function of the Endolysosomal/Lysosomal System
… study, endolysosomes which formed by fusion of late endosomes with lysosomes and are thought to be the predominant site of hydrolytic activity, were further characterised. Using live cell imaging and fluorescent labelling, the proportion of endolysosomes in the total pool of lysosomes was …
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Yeast Dynamin Functions with Clathrin at the Golgi
… endocytosis, but also traffic from the Golgi to endosomes. In this study, the interdependency between Vps1 and clathrin was investigated. Subcellular localization of clathrin was at the Golgi, early and late endosomes, implicating clathrin in multiple trafficking pathways within the cell. Vps1 …
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Functional Connection Between Yeast Dynamin and Retromer at the Endosome
<p>Intracellular trafficking from the late endosome to Golgi in cells is termed retrograde transport, essential for recycling of important macromolecules including cell membrane receptors. Retrograde transport is regulated by a family of proteins known as the "Retromer” composed of 5 VPS proteins …
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Investigating protrudin’s role in neuroprotection and axon regeneration in the central nervous system
… does not have any effect on the transport of late endosomes in CNS neurons — despite evidence for this mechanism in other cell types — due to the absence of key adaptor protein FYCO1 in mature neurons. It also does not have any substantial effect on dendritic spine morphology, so it does not …
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PROTEINi screens identify ORP9 as a novel regulator of KRAS-driven signalling
… into target organelles, such as the Golgi and late endosomes. ORP9 lipid cargoes include phosphatidylserine and cholesterol, which can be transferred out of the ER membrane in exchange for PI4P, which is then dephosphorylated by the ER-resident Sac1 phosphatase. TAT-OPX and TAT-HPX caused …
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Novel findings on cellular trafficking and targeting for granule storage of neutrophil elastase, a multifunctional effector molecule of innate immunity
… rafts, and possible conversion to mature NE in late endosomes. The inhibition of proNE’s activation into mature NE was accompanied by the accumulation of proNE, suggesting a requirement for activation before granule targeting. This research provided new perspectives on the cellular trafficking …
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Endosomal Trafficking and Exosomal Secretion of Egfrviii
… internalized into the intraluminal vesicles of late endosomes / multivesicular bodies (MVBs) but does not follow the canonical pathway by which wild-type EGFR is degraded following MVB fusion with lysosomes. I determined that EGFRvIII is secreted on exosomes, the intraluminal vesicles that are …
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The Hydrophobic Handoff Between NPC2 and the N-Terminal Domain of NPC1 in the Export of Cholesterol from Lysosomes
Low density lipoproteins (LDL) and related plasma lipoproteins deliver cholesterol to cells by receptor-mediated endocytosis. The lipoprotein is degraded in late endosomes and lysosomes where its cholesterol is released. Egress of cholesterol from late endosomes and lysosomes (hereafter referred to …
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The Role of Membrane Domains In Protein and Lipid Sorting During Endocytic Traffic
… for PM recycling of raft-preferring cargo from late endosomes. I implicate Rab3 as a central regulator of this pathway and show that the Rab3 family is essential for PM homeostasis, as abrogation of all four members of the Rab3 family disrupts PM recycling of lipid raft associated proteins. The …
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Anchoring Energy of Dengue E Protein into Host Membranes and Quaternary Assembly Depend upon Lipid Composition
… E trimers that facilitate membrane fusion with late endosomes. The fusion mechanism is not fully understood, but poses as a key target for inhibiting the viral infection pathway. An important aspect of fusion is the requirement of anionic lipids in the endosomal membrane. This study aims to …
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Identifying the Role of Phospholipase D1 and Phosphatidic Acid in Exocytosis
… during exosome secretion from multivesicular endosomes (MVEs) in A549 cells. PLD1 localizes to late endosomes, especially MVEs. PLD1 is also present on MVEs during the entire process, but it is only required to produce PA on docking MVEs. PLD1 inhibition also increases the density of lysosomes …
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Illuminating Organellar and Molecular Organization of the Endomembrane System
… junctions and show that it is in part regulated by F-actin. This gives clues about how cellular activities of endoplasmic reticulum-plasma membrane junctions may be regulated because their spatial organization dictates the extent and location of these cellular activities. Then, I examine …
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