Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"late endosome"”.
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Vps1's Implication on Late Endosome-to-Vacuole Traffic
… of FM4-64 puncta that colocalize with a late endosomal marker (GFP-Pep12), indicating a slower late endosome-to-vacuole traffic. In addition, Vps1 was found to genetically interact with Vps36 and Ypt7, which are involved in the late endosome-to-vacuole traffic. Protein's functional role …
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Characterization of Class D VPS Proteins
… to control anterograde traffic between the late Golgi and late endosome. Although most of these proteins have homologues of known function in other systems, two exceptions are the Vps3p and Vps8p proteins. Analysis of Vps3p reveals that it is associated with a highdensity structure, possibly …
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Investigations into the Nature of the Endosomal System in Plasmodium falciparum
… responsible for the vast majority of malaria-related deaths. During the asexual intraerythrocytic stage, the parasite must transport newly synthesized proteins and endocytosed cargo to a variety of organelles, many of which are formed de novo and have no human equivalent. This process in …
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Molecular Mechanisms of Inward and Outward Budding From Multivesicular Endosomes
… and arrive at the limiting membrane of the late endosome/multivesicular body (MVB) is dictated by whether they remain on the limiting membrane, bud into internal MVB vesicles, or bud outwardly from the membrane. The molecular details underlying the disposition of membrane proteins that …
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Endocytic Trafficking of The Amyloid Precursor Protein In Rat Cortical Neurons
… of Aβ peptides that are secreted and accumulate extracellularly. Despite considerable progress towards understanding APP processing and Aβ aggregation, the mechanisms underlying endosomal production of Aβ peptides and their secretion remain unclear. Using endosomes isolated from cultured …
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Yeast Dynamin Functions with Clathrin at the Golgi
… endocytosis, but also traffic from the Golgi to endosomes. In this study, the interdependency between Vps1 and clathrin was investigated. Subcellular localization of clathrin was at the Golgi, early and late endosomes, implicating clathrin in multiple trafficking pathways within the cell. Vps1 …
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Functional Connection Between Yeast Dynamin and Retromer at the Endosome
<p>Intracellular trafficking from the late endosome to Golgi in cells is termed retrograde transport, essential for recycling of important macromolecules including cell membrane receptors. Retrograde transport is regulated by a family of proteins known as the "Retromer” composed of 5 VPS proteins …
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Quantitative Studies of Intracellular Trafficking of Two Classes of Resident Golgi Apparatus Proteins
… unique Golgi resident protein that cycles in the late endosome bypass pathway. Using the published method of analysis and techniques developed during the initial project, the anterograde and retrograde transport kinetics of this Golgi protein were determined and used to develop a compartmental …
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Biodistribution of Modified Extracellular Vesicles In Normal and Tumor-Bearing Animal Models
… nm. They are formed by early budding into early endosomes then released by the cell from late endosome. The content of EVs includes proteins, mRNA, miRNA, and DNA derived from the original cell where they were released. Due to the membrane structure of EVs, which resemble the original cell, and …
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Recruitment and function of ORP1L on the Coxiella burnetii parasitophorous vacuole
… host protein ORP1L (Oxysterol binding protein-Related Protein 1, Long) during infection. ORP1L is a multi-functional cholesterol-binding protein involved in late endosome/lysosome (LEL) trafficking, formation of membrane contact sites between LEL and the endoplasmic reticulum (ER), and …
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The Role and Mechanism of Enhanced Met Expression in Pancreatic Cancer
… Met protein levels are posttranslationally regulated in a subset of pancreatic cancers; specifically, that Met down regulation is delayed. My studies demonstrate that Met down regulation is delayed in a number of pancreatic cancer cell lines and that at least two separate mechanisms are …
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Identifying the Role of Phospholipase D1 and Phosphatidic Acid in Exocytosis
… during exosome secretion from multivesicular endosomes (MVEs) in A549 cells. PLD1 localizes to late endosomes, especially MVEs. PLD1 is also present on MVEs during the entire process, but it is only required to produce PA on docking MVEs. PLD1 inhibition also increases the density of lysosomes …
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Investigation of the intracellular trafficking pathways of the dermonecrotic toxin family
… of the dermonecrotic toxin family composed of related AB toxins whose members induce dermonecrosis upon injection into animal skin. The dermonecrotic toxins Pasteurella multocida toxin (PMT) from Pasteurella multocida (P. multocida), cytotoxic necrotizing factors 1, 2, and 3 (CNF1, 2 and 3) from …
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Membrane trafficking of ATP-sensitive potassium channels
… of [ATP]/[ADP] within the cell are known to regulate the activity of channels, but very little is known how the number of channels at the cell surface is regulated. The number of channels in the plasma membrane could be regulated in two ways; firstly by regulating the overall population of …
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Immunostimulatory properties and mechanisms of action of encapsulated methylated cpg oligodeoxynucleotides
… oligodeoxynucleotides (ODN) containing unmethylated CpG motifs are powerful stimulators of innate as well as adaptive immune responses, exerting their activity through the triggering of the endosomally localized TLR9 by a poorly understood mechanism. The immunopotency and broad range of …
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The Role of the YXXΦ Motif in CD82 Trafficking and Function
… of tissues, but its expression becomes down-regulated or lost in a majority of metastatic tumors. It inhibits tumor metastasis without affecting primary tumor growth. Cancer patients whose tumors contain CD82 exhibit minimal metastasis. We know that CD82 functions as a tumor metastasis suppressor …
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Predicting angiogenic receptor trafficking and signaling via computational systems biology
… internalization. Thus, endocytosis may regulate VEGFR1 signaling; indeed, intracellular-based receptor signaling has recently emerged as a key component in mediating cell responses for receptor tyrosine kinases (RTKs). However, how endocytosis fundamentally mediates signaling for any RTK …
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Endosomal Trafficking and Exosomal Secretion of Egfrviii
… internalized into the intraluminal vesicles of late endosomes / multivesicular bodies (MVBs) but does not follow the canonical pathway by which wild-type EGFR is degraded following MVB fusion with lysosomes. I determined that EGFRvIII is secreted on exosomes, the intraluminal vesicles that are …