Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 92 for “"laser scanning microscopy"”.
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Confocal laser scanning microscopy of nanoparticles applied to immunosorbent assays
… ng/ml, respectively. The application of confocal microscopy in kinetic analysis of the antigen-antibody reaction of the human IgG model was studied for homogeneous and heterogeneous formats and two fluorescent labels antibodies (FITC and QDs).
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Three-Dimensional Volume Reconstruction From Fluorescent Confocal Laser Scanning Microscopy Imagery
The broader impact of my work is in providing the algorithms in a form of web-enabled tools to the medical community so that medical researchers can minimize laborious and time intensive 3D volume reconstructions using the tools and computational resources at NCSA.
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Confocal Laser Scanning Microscopy As A Tool For The Investigation Of Tetracycline Fluorescence In Archaeologicalhuman Bone
… of remains postmortem. Standard epifluorescence microscopy is the most common tool employed in the analysis of these labels. Though valuable, this technique is limited by its inability to penetrate bone three-dimensionally and its inclusion of out-of-focus light, possibly disrupting accurate …
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Development of a head-mount fiber scanning system for imaging in vivo
… from nerve tissue by application of two-photon laser scanning microscopy and its miniaturization for in vivo imaging in freely moving animals. The construction of a laser with enhanced repetition rate and the design of a novel non-resonant fiber scanning technique is emphasized. The increased …
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Test Development and Material Characterization of Hot Poured Crack Sealant at Low Temperature
… the sealants were also investigated by confocal laser scanning microscopy (CLSM), environmental scanning electron microscopy (ESEM), gel-permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), and thermo-gravimetric analysis (TGA). These techniques reveal the composition …
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Towards understanding multiscale articular cartilage mechanics
… A popular experimental approach employs laser scanning microscopy coupled with mechanical testing systems capable of acquiring 3-D images of tissue subjected to controlled mechanical conditions. By comparing tissue volumes in different mechanical states, micro-scale deformation can be …
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Novel Endodontic Disinfection Approach Using Nanotechnology
… used chemical modalities using confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) as analytical tools. We hypothesized that iron oxide NP with enzyme-like (peroxidase) activity catalyzes H2O2 to promote bacterial killing within dentinal tubules (DT) via in …
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Polarization Controlled Coherent Anti-Stokes Raman Scattering Microscopy for Determination of Structural Order of the Myelin Sheath
Coherent Anti-Stokes Raman Scattering (CARS) is a laser-scanning microscopy technique that generates a strong label-free signal in lipid. The myelin sheath surrounding nerves is up to 80% lipid, and therefore is an ideal candidate for CARS imaging. The long lipid chains forming the myelin wraps …
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Multiplexed nanoparticle-based immunoassays
… fluorophores and nanoparticles. Epi-fluorescence microscopy, confocal laser scanning microscopy and programmable array microscopy were used to detect signals from mixtures of conventional organic fluorophores, quantum dots and silica nanoparticles doped with europium, samarium and terbium in …
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Microbiological Study of Bacterial Load Reduction in the Root Canal System Utilizing a Novel ErYagLaser
… to evaluate the effectiveness of a novel Er:Yag laser-activated irrigation technique in reducing bacterial load in root canals. In this study, mandibular molars are prepared and cultured with Enterococcus faecalis, and experiment was perform to test whether laser-activated irrigation could …
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Design, fabrication and testing of an acoustic resonator-based biosensor for the detection of cancer biomarkers
… assessed using water contact angle, atomic force microscopy and confocal laser scanning microscopy. Functionalized biosensors are then used to quantify the concentration of known proteins marker in both a purified medium and a physiologically-relevant medium.
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Material, acoustic, and mechanical properties of mosquito and midge antennae
… hearing in insects. To this end, Confocal Laser Scanning Microscopy (CLSM), Finite Element Modelling (FEM) and Laser Doppler Vibrometry (LDV) were employed. The combination of the first two allows, in absence of prior knowledge of the material properties of mosquito or midge antennae, to …
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Glutathione Dynamics in Arabidopsis Seed Development and Germination
… glutathione was measured in situ by confocal laser scanning microscopy. Stress in the form of natural and accelerated ageing, and germination at high and low temperature and at low water potential was applied to both WT and anGR2 seeds to test vigor. Results show similar glutathione levels and …
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Nanoscale surface modification of wood veneers for adhesion
… for polyelectrolyte adsorption. Confocal laser scanning microscopy (CLSM) and Environmental Scanning Electron Microscope (ESEM) were used to characterize adsorbed LbL multilayers of poly(acrylic) acid (PAA) and poly(allylamine hydrochloride) (PAH). Cross-linking between PAA and PAH at …
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Compact Femtosecond Lasers for Multiphoton Imaging
… is one of the main development directions in Laser Scanning Microscopy. A simplified way to assess suitability of a given laser for MPI applications is a figure of merit (FOM) approach based on peak power and average power of the particular excitation laser used. The first systematic studies …
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Evolution and Anatomy of Early Terrestrial Arthropods: Insights from the Rhynie Chert
… their first steps. This thesis employs confocal laser scanning microscopy (CLSM) to investigate the anatomical and functional adaptations of Rhynie arthropods, addressing key questions about the evolution of arthropod terrestrialisation and the morphological innovations that enabled life to …
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Three dimensional surface texture analysis for characterisation of enamel erosion
… and surface texture was measured using confocal laser scanning microscopy in combination with MountainsMap® surface analysis software. For the unpolished samples, only surface texture was measured. Statistical analyses were carried out with repeated measures one-way ANOVA. For the polished …
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Biofilm formation in clinical and laboratory strains of Escherichia Coli and Campylobacter Jejuni
… method, crystal violet staining and confocal laser scanning microscopy. The results for the E. coli laboratory isolatesi ndicated a decreasein biofilm fonnation in Luria-Bertani (LB) broth supplemented with lysed horse blood, whole and lysed human blood, human plasma, human serum and whole and …
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Biomolecular effects and bioclinical applications of PARPs inhibitors
… By western analysis and confocal laser scanning microscopy (LSM), we analysed the effects of PJ-34 on PARP-1 expression, phospho-ERK and phospho-Elk-1 activation. The effect of MEK (mitogen-activated-protein-kinase-kinase) inhibitor PD98059 …
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