Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"laser microdissection"”.
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The Use And Development Of Laser Microdissection To Separate Spermatozoa From Epithelial Cells For Str Analysis
… for DNA typing. This report describes the use of laser microdissection (LMD) for the separation of pure populations of spermatozoa from two-donor cell mixtures. In this study, cell separation was demonstrated by microscopic identification of histologically stained spermatozoa and female buccal …
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Chromosomal localization of a proinsulin transgene inserted with a transposon-based vector into Japanese quail, Coturnix coturnix
… of Teflon-coated coverslip slides to facilitate laser microdissection of 0.5 ƒÝm samples, and chromosomal identification of proinsulin transgene insertions by laser microdissection and nucleotide sequence from G2 Japanese quail. The 28S rDNA was found on a macrochromosome and a microchromosome …
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The pathogenesis of classical Hodgkin lymphoma : investigation of possible viral pathogens and recurrent chromosomal imbalances
… cHL case, single HRS cells were isolated using laser microdissection. DNA was extracted then amplified by degenerate oligonucleotide primer polymerase chain reaction. MV and PyV genomes were not detected within cHL biopsies. Recurrent chromosomal imbalances were confirmed within the cHL-derived …
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THE INFLUENCE OF THE MICROENVIRONMENT OF HEALTHY AND TUMORAL CELLS
… combination of decellularization procedures and Laser Microdissection (LMD) and tested against tumoral rat cell line. As reference, we also performed force spectroscopy experiments using AFM spherical probes functionalized with the two main ECM proteins, laminin and fibronectin. Native ECM probes …
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Comparative genomics of chromosomal rearrangements in malaria mosquitoes
… species. We have developed a new protocol for laser microdissection and whole genome amplification of polytene chromosomal fragments to obtain DNA for sequencing and assembly. The chromosomal regions spanning both breakpoints of the 2La in A. arabiensis and A. merus were laser microdissected …
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Investigations of equine sarcoids and bovine papillomavirus in Western Canada
… were dissected into tissue compartments using laser microdissection followed by 2 forms of BPV DNA amplification, PCR and isothermal loop mediated amplification. BPV DNA was more often located in the epidermis of non-sarcoid skin conditions than in sarcoids. In addition, areas of inflammation …
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Effect of GABRA2 expression in the central nucleus of the amygdala on anxiety and alcohol's anxiolytic capacity in C57BL/6J mice
… GFP was quantified, the CeA was collected via laser microdissection, and α2 protein was quantified via ELISA. In mice expressing GFP in the CeA, α2 protein concentrations were lower for Virus mice relative to Control mice. The EPM was anxiogenic, and alcohol was found to be anxiolytic. In naïve …
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Clonal proliferation of hepatocytes during chronic hepatitis B virus infection.
… and –negative hepatocytes isolated by laser-microdissection. Heterogeneous expression of cellular proteins was detected using IMS in hepatocytes with apparently normal histology. These results indicate that clonal proliferation of hepatocytes with survival advantage does occur in the …
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Mutational processes in normal human tissues
… which somatic mutations can be called. Using laser-capture microdissection to isolate individual crypts followed by whole-genome sequencing, I characterised somatic mutation rates and mutational signatures in the small intestine, and identified the frequent presence of a mutational process …
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Natural genetic variation and gene expression patterns underlying lateral shoot (tiller) development in barley (Hordeum vulgare L)
The main shoot and other above-ground tissues develop from the shoot apical meristem (SAM), including axillary meristems (AXM) from which lateral branches develop. In barley, lateral branches called tillers contribute directly to grain yield and define shoot architecture. Two studies were completed …
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Tracking climate signals in tropical trees
… isotope analysis of tree rings. Two different UV-laser-based microscopic dissection systems were evaluated as a novel sampling tool for high-resolution stable isotope analysis. Furthermore, an improved procedure of tree-ring dissection from thin cellulose laths for stable isotope analysis was …
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Regenerationspotenzial CD133+-hämatopoetischer Progenitorzellen der humanen Nabelschnur beim Nierendefekt im Mausmodell
Eine Schädigung des renalen Tubulusgewebes ist in den meisten Fällen irreversibel. Persistierender Abbau oder chronische inflammatorische Prozesse führen letztendlich zum Nierenversagen. Bisher sind die therapeutischen Optionen auf eine Dialyse beziehungsweise Organtransplantation begrenzt. In den …