Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 16 of 16 for “"laser induced fluorescence detection"”.
-
Nanoscale protein analysis utilizing capillary electrophoresis and laser-induced fluorescence detection
… volume sampling and low protein concentration detection. Furthermore, one of the major trends in high throughput screening is the growing replacement of technologies that depend on radioactivity to generate a signal with those that rely on fluorescence. This trend towards non-radioactive …
-
Neurotransmitter and Neuromodulator Analysis Using Capillary Electrophoresis With Laser -Induced Fluorescence Detection
A variety of results on these neurotransmitters/neuromodulators have been elucidated using CE-LIF methods. Experimental results of the D-Asp study are consistent with the hypothesis that D-Asp is involved in cell to cell communication in Aplysia californica. In addition, D-Glu is found in the …
-
ANALYSIS BY CAPILLARY ELECTROPHORESIS WITH LASER-INDUCED FLUORESCENCE DETECTION: FROM SMALL MOLECULES TO WHOLE CELLS
Capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) is a versatile and sensitive analytical tool with many potential applications. Its advantages over other chromatographic methods include minimal solvent and sample requirements, low waste volumes, simple instrumentation, …
-
CAPILLARY ELECTROPHORESIS ANALYSIS OF SINGLE-STRANDED DNA APTAMERS AND THEIR INTERACTIONS WITH PROTEIN TARGETS AND CARBON DOTS
… Capillary electrophoresis with UV/Vis absorbance detection and laser-induced fluorescence detection (CE-UV and CE-LIF) was used to explore whether or not carbon dots (CDs) could significantly change the mobility of ssDNA samples upon noncovalent interaction. Preliminary observations revealed …
-
Investigation of biomarkers for noninvasive early diagnosis of cancer
… high-performance capillary electrophoresis with laser-induced fluorescence detection (HPCE-LIF) in the first study. The results showed that 5 pteridine molecules were elevated in cancer compared to non-cancer urine samples. The second study investigated the levels of proline, kynurenine, uracil, …
-
Biofunctionalization of Submicrometer Pores and Mass Limited Sample Manipulations in Three-Dimensional Hybrid Microfluidic/nanofluidic Devices
… scanning electron microscopy and matrix-assisted laser desorption/ionization (MALDI) with time-of-flight mass spectrometry (ToF-MS). The NCAMs with molecular recognition capability not only can be used as a biological sensing component but also can be used as a tool for investigating specific …
-
Characteristics and Performance of Novel Squarylium Dyes as Fluorescent Probes for the Analysis of Proteins and Viruses by CE-LIF
… fluorescent labels to aid biomolecule detection. Noncovalent binding of dyes through hydrophobic, electrostatic, and other interactions with biological molecules can result in improved sensitivity by assays using capillary electrophoresis with laser induced fluorescence detection …
-
A Capillary-Based Microfluidic System for Immunoaffinity Separations in Biological Matrices
… separations in biological matrices with laser-induced fluorescence detection of labeled antigens or antibodies. The laboratory-constructed device was assembled from two micro syringe pumps, a microchip mixer, a micro-injector, a diode laser with fused-silica capillary flow cell, and a …
-
Single cell analysis by capillary electrophoresis with laser-induced native fluorescence detection
… were analyzed by capillary electrophoresis with laser-induced fluorescence detection (CE-LIF). This technique was chosen due to its high separation efficiencies, small sample volumes and sensitive detection. Native fluorescence was used, in which the analyte was not tagged with a fluorophore. …
-
Investigation of Novel Microseparation Techniques
… evaluated with the aid of a home-made scanning laser-induced fluorescence detection system. The fiber-based CCSA EFGF devices gave poorer performance compared with glass capillary based devices. Porous glass capillary-based EFGF devices could focus single proteins and separate mixtures of two to …
-
DEVELOPMENT OF MICROCHIP ELECTROPHORESIS BASED METHODS TO PROFILE CELLULAR NITROSATIVE STRESS
… diseases. A method for the simultaneous detection of prooxidants and antioxidants associated with nitrosative stress in biological samples would be beneficial for better understanding of their role in disease states. Therefore, in this dissertation a separation-based approach is described …
-
New Instrumentation and Methods for Analyzing Biological Compounds for Drug Discovery and Discovering Novel Disease Markers
… uses a sheath flow cuvette combined with laser induced fluorescence detection for high sensitivity measurements of single vesicles. 200 nm and 400 nm DOPC vesicles doped with varying amounts of PE-CF lipid were measured. Single vesicle resolution was obtained through optimization of the …
-
PROTEIN AND OPIOID ANALYSIS BY CAPILLARY ELECTROPHORESIS WITH LASER INDUCED FLUORESCENCE AND MASS SPECTROMETRY DETECTION
… methods through the use of CE with laser-induced fluorescence detection (LIF). Optimal labeling and separation methods utilizing four novel asymmetric squarylium dyes as universal protein probes are presented herein. SQHN-1, -1a, and -1b have the same asymmetric structure with a …
-
Development of capillary electrophoretic-based techniques to analyze doxorubicin in tissues, cells and subcellular fractions.
… Using this technique coupled with MEKC-LIF (laser-induced fluorescence detection), DOX was detected and quantified, in both tumor and non-tumor regions in resected human livers. A MEKC method was developed to monitor the incorporation, transformation and subcellular metabolism of a DOX …
-
Screening of algal cells using microdroplet technology
… cell suspension prior to the encapsulation. A fluorescence-activated droplet sorting platform was used to detect the fluorescence of encapsulated algal cells. Following modifications to the optics of a previously used platform, the fluorescence measurement uncertainty was reduced from 87% to …
-
Amino acid racemase responsible for the biosynthesis of D-aspartate and D-serine signaling molecules in Aplysia californica central nervous system
ABSTRACT While dogma states that animals use L-amino acids, several D-amino acids have been found in animals including humans since the 1980s. Two D-amino acids have attracted significant research interests because of their cell-cell signaling functions in animal nervous systems. D-serine (D-Ser) …