Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 33 for “"laser capture microdissection"”.
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KGF-1 and KGF receptor expression in human periodontal disease and in vitro microwounding-associated-ligand-independent KGFR activation
… tissues, we used immunohistochemistry and laser capture microdissection with RT-PCR to analyze KGF-1 and KGFR expression and localization. To study ligand independent KGFR phosphorylation, KGFR internalization along the wound edge was imaged using immunohistochemical staining and KGFR …
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The development and function of thymic microenvironments
… of the thymus has been highlighted by employing laser capture microdissection and studying thymus microenvironments in mice deficient for particular chemokine related signalling pathways. Overall this study has provided novel insight into the development of the thymic cortex and will help to …
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Investigating Invasion In Ductal Carcinoma In Situ With to pographical Single Cell Genome Sequencing
… invasive regions within the same sample. While laser capture microdissection studies of DCIS-IDC examined the relationship between the paired in situ (DCIS) and invasive (IDC) regions, these studies were either confounded by bulk tissue or limited to a small set of genes or markers. To overcome …
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Molecular characterization of CLE peptide mimicry during cyst nematode pathogenesis
… of the syncytium. Ongoing studies will utilize laser capture microdissection coupled with mRNA-Seq to identify downstream signaling cascades after nematode CLE perception. The results of these studies will contribute to our understanding of how plant-parasitic nematodes interact with their host …
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Identification of intravasation sites for metastatic biomarker development
… approach for sites of intravasation, followed by laser capture microdissection coupled to proteomics for molecular assessment of the changes at these sites of breached vasculature. Importantly, by working with biobanks and clinical collaborators, identified markers of intravasation will be …
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Molecular Control of Tooth Mesenchymal Stem Cell Activation
… cell (MTAC) containing regions were undertaken. Laser capture microdissection and subsequent comparative analysis of MSC marker expression between populations was performed. Investigation of the molecular mechanisms governing MSC activation and maintenance was undertaken. Specifically, the role …
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Genetic regulation of maize floral development - insights from the transcriptome and translatome
… and lower floral meristems in maize ear using laser capture microdissection coupled with RNA sequencing. Differentially expressed genes were involved in hormone regulation, cell wall, sugar and energy homeostasis. Furthermore, cell wall modifications and sugar accumulation differed between the …
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Role of Pro-Apoptotic Bcl2-Homology-3 Domain (BH3)-Only Proteins in the Mutant SOD1 Mouse Model of ALS
… manner to cause motor neuron death. We utilized laser capture microdissection (LCM) to collect highly enriched populations of spinal motor neurons from wild type vs. SOD1 mutant mice. RNA was then isolated from the captured motor neurons and used for quantitative real time PCR analysis of …
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Spatially mapped gene expression analysis from tissue
… or in situ hybridization (ISH). In addition, laser capture microdissection (LCM) followed by RT-qPCR of the locally captured tissue has also been used to study these spatial variations at a molecular level. But, all of the above techniques are plagued with different issues such as low …
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Somatic evolution in human blood and colon
… unit, isolating single colonic crypts through laser capture microdissection. 570 colonic crypts from 42 individuals were whole genome sequenced. We describe the burden and pattern of somatic mutations in these genomes and their variability across and within different people, identifying some …
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The Liver-Derived Endocrine Hormone FGF21 Alters Metabolism and Diurnal Behavior via the Nervous System
… this idea, I utilized anatomically-guided laser capture microdissection followed by quantitative, real-time PCR to profile expression of the FGF receptor/co-receptor family in specific hypothalamic nuclei of mice. Surprisingly, the FGFR1-IIIc/βKlotho complex is found in the suprachiasmatic …
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The Role of Bone Morphogenetic Protein (BMP) Signalling in Lung Cancer and Lung Regeneration
… Early lung cancer tumours were isolated by laser capture microdissection from Kras G12D mouse lungs and microarray analysis was carried out on RNA from the purified populations of cells. Microarray and qPCR analyses revealed differential BMP pathway expression during early tumour formation. …
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Regulation of cellular growth and identification of stromal gene signatures in breast cancer
… of cancer- and stroma-specific genes using laser capture microdissection on FFPE triple negative breast cancers. Gene expression microarrays of these samples revealed compartment specific gene expression and enabled identification of stromal-specific gene signatures with tumor-predictive …
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Establishing models of and characterising the genomic context of neutrophil hypersegmentation
… the current work outlines the optimisation of a laser capture microdissection (LCM) technique for the isolation of neutrophils, based on their morphologic phenotype, to permit RNA sequencing. This optimisation has led to the first description of the transcriptome of microdissected neutrophils, …
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Repression of Protein Kinase C Delta in Human Squamous Cell Carcinomas by Ras, Fyn and NF-Kappa B Signaling
… of PKC-delta loss in human SCCs, we used Laser Capture Microdissection to isolate cells for RNA and DNA analysis from 3 normal epidermises and 14 human SCCs with low PKC-delta protein. Using this more selective approach, we found the tumor suppressor PKC-delta is lost at the mRNA level in …
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Quantitative cellular and molecular imaging of the intact tumor microenvironment.
… that can be grossly summarized as: (1) Antigen capture for presentation (dendritic cells, DCs) or for degradation (macrophages); (2) Tissue repair (macrophages) and (3) effector function (mast cells, monocytes, granulocytes). However, the functional status of myeloid cells in human tumors and …
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Somatic Mutations in Ageing and Degenerative Disease
… rheumatoid arthritis and osteoarthritis. Using laser capture microdissection, we isolated 2000 microbiopsies of intimal lining, sublining, and lymphocytes for whole exome sequencing. Using these results we show that the synovium is a mostly polyclonal tissue with the capacity for large clonal …
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EFFECT OF NICOTINE ON LUNG S-ADENOSYLMETHIONINE AND PNEUMOCYSTIS PNEUMONIA DEVELOPMENT
… the possibility of tissue specificity. Using laser capture microdissection (LCM), we collected samples of lung alveolar regions (site of infection) and respiratory epithelium for controls. We found nicotine to cause increased ODC activity in alveolar regions but not airway epithelium; we …
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Mechanisms for Osteoblast and Osteocyte Initiation and Sustainment of Bone Formation in Young-Adult and Aged Mice
… Wnt1 in osteocyte-enriched intracortical bone by laser capture microdissection and microarray analysis. These results were important in demonstrating the presence of Ngf in osteocytes, and in suggesting the role of Wnt1 as a potential signaling molecule between osteocytes and osteoblasts which are …
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Transport processes in the arbuscular mycorrhizal symbiosis
… with a significantly altered expression level in laser capture microdissection-collected arbuscules compared to extraradical tissues were identified. Among them, six previously unknown specifically regulated potential transporter genes were found. These are likely to play a role in the nutrient …
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