Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"larval zebrafish"”.
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Investigating the role of macrophages in larval zebrafish heart regeneration
… and this can lead to heart failure. In contrast, zebrafish can completely regenerate their hearts after cardiac injury via dedifferentiation and proliferation of surviving cardiomyocytes. Macrophages are a required cell type for successful zebrafish heart regeneration and so may offer a roadmap to …
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A Larval Zebrafish (Danio Rerio) Model of Adherent-Invasive Escherichia Coli Infections
… to IBD <em>in vivo</em> remains unclear. Here a larval zebrafish (<em>Danio rerio</em>) model of AIEC was established, which facilitates the study of the role of pre-existing inflammation, and host- and pathogen- genetic factors during IBD pathogenesis. Paramecium caudatum, a natural prey of …
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Characterization of selected single and convergent stimuli-induced behaviors in larval zebrafish
… setups, which were used to characterize larval zebrafish behavior at various stimuli parameters. Tested stimuli was chosen from the perspective of eventual fluorescent neural imaging, so as to be both compatible with, and aware of the stimulating aspects of, a conventional florescence …
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Imagining the Voltage of Neurons Distributed Across Entire Brains of Larval Zebrafish
… of the brain, even for small animals like the larval zebrafish. To address this challenge, this thesis presents a high-speed remote scanning light-sheet microscope capable of imaging GEVI-expressing neurons distributed throughout entire brains of larval zebrafish at a volumetric rate of 200.8 …
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Exploring combined effects of arsenic and selenium exposure on larval zebrafish (Danio rerio)
… on aquatic life. To investigate this interplay, zebrafish embryos were exposed to As alone (as arsenite) or in combination with two different chemical forms of Se including As 120 μg/L alone (as arsenite), As 120 μg/L + Se 25 μg/L (as selenite), As 120 μg/L + Se 120 μg/L (as selenite), and As 120 …
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Characterising the functional homology of central nervous system drug targets in larval zebrafish (Danio rerio)
… in fish predicts functional effects, using larval zebrafish (Danio rerio) as a model system. The study focuses on nine widely prescribed and environmentally relevant compounds: three anticonvulsants (carbamazepine, lamotrigine, gabapentin), three antidepressants (venlafaxine, escitalopram, …
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The effects of noise exposure and genetic background on auditory-evoked behaviors in larval zebrafish
… behaviors? I addressed this question in the larval zebrafish (Danio rerio). Zebrafish have been a powerful genetic model species used in the study of development of auditory structures and exhibit a well-studied and robust escape response when presented with a loud acoustic stimulus. I used …
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Development of Novel Methods to Study Host-Microbe Interactions In The Larval Zebrafish Gastrointestinal Tract
… study of intestinal physiology and pathology. Zebrafish larvae, possessing optical transparency and genetic tractability, offer an accessible and clinically relevant model for investigating dynamic events in the intestine via time-lapse imaging. In the first part of this work, I discuss our …
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The Guidance of Olfactory Sensory Axons to Identifiable Protoglomeruli in the Larval Zebrafish Olfactory Bulb
… later in development. The three day old larval zebrafish olfactory bulb contains 12 stereotyped, identifiable protoglomeruli, rendering it a good system to investigate mechanisms of initial axonal targeting in the bulb. In this thesis, I describe the generation of transgenic zebrafish …
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ELUCIDATING THE ROLE OF MIR-10A/B IN THE WOUND HEALING PROCESS IN LARVAL ZEBRAFISH
… the regulatory role of microRNA-10 (miR-10) in larval zebrafish caudal fin regeneration, a model with broad implications for the future of regenerative medicine. Building on prior research from the lab, which indicated that miR-10 knockdown impairs regeneration, we verified this finding through …
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Influence of parental swimming stamina on the cardiac and metabolic performance of larval zebrafish (Danio rerio).
… cardio-respiratory support for locomotion in larval fishes has not been determined. Is the expression of the suite of parental traits enabling superior swimming stamina in their offspring dependent upon their achieving juvenile/adult morphology, or does it appear earlier in their larvae? To …
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Technological Innovation and Integration of Whole Brain Imaging, Olfactory Stimulation, and Correlative Microscopy in Larval Zebrafish
… richer and more contextual understandings of the larval zebrafish's brain. In this work we show novel light field microscopy algorithms that allow us to reconstruct 3D images from 2D micrographs with improved resolution to enable high-frame-rate recordings of whole-brain neural activity. We …
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Characterization of an Enhancer Upstream of Msx3 and its Role in Development of the Neural Tube of Embryonic and Larval Zebrafish
… and proliferation in the neural tube. In zebrafish, three msx genes are expressed in the neural tube: msx1a, msx1b, and msx3. The Akimenko lab has identified a potential enhancer of msx3 called Fragment C that drives expression in the dorsal neural tube. We hypothesized that Fragment C is …
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Behavioral analysis and protein folding in zebrafish larvae
The zebrafish, Danio rerio, is a very important vertebrate model organism with growing popularity in biology and neuroscience. My thesis work covered two topics centered on zebrafish. Chapter 1 and Chapter 2 focused on the swimming behavior of the animal. This topic is of great interest because …
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Towards an understanding of the developmental zebrafish (Danio rerio) proteome and impacts of age-specific sensitivities with a model toxicant.
… among future studies. Early life stage zebrafish have gained popularity as an animal alternative with incorporation into standardized methods (such as Organisation for Economic Cooperation and Development test guideline no. 236) to become arguably the most important alternative …
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Towards mapping spatial transcriptome of an entire vertebrate brain
… for understanding this essential organ. Zebrafish, for their modest brain size and transparency in the larval stage, serve as a model organism for whole-brain in vivo imaging and modeling. While calcium imaging generates substantial amounts of neural activity data, the lack of molecular …
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A Signal to Divide: Apoptotic Extracellular Vesicles as Carriers of Mitogenic and Immunogenic Signals
… visualizes and characterizes AEVs in larval zebrafish and MDA-MB-231 cells to understand apoptosis-induced proliferation mechanisms by assessing proteomic contents and studying real-time dynamics of AEVs. In both systems, I found AEVs to be enriched in molecules that can modulate cell …
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Tools for Mapping the Links Between Stimuli, AffectiveStates, and Behavior through Whole-Brain Imaging inZebrafish Larvae
… functional architecture. In this pursuit, zebrafish larvae emerge as an ideal model for whole-brain imaging due to their transparency, small size, genetic manipulability, rapid development, high reproducibility, Recent advances in protein engineering and fluorescence microscopy have …
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On the High-Dimensional Geometry of Neuronal Population Dynamics
… processing. Next, we switched our focus to the larval zebrafish in order to ask how highdimensional whole-brain dynamics produce population codes that are robust yet flexible enough to generate variable behaviors. To do so, we honed in on a regime of visual object size — between those that …
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Neuromast Optogenetics Reveals Rules of Spatial Encoding in the Zebrafish Lateral Line
… study, I use the posterior lateral line (pLL) of larval zebrafish to address this question. In the wild, the pLL must differentiate among a variety of hydrodynamic stimuli. However, understanding how fish distinguish between different water-flow stimuli has been challenging due to difficulties in …
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