Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"large-scale chromatin"”.
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Effects of Transcriptional Activators on Large-Scale Chromatin Structure
*This dissertation is multimedia (contains text and other applications not available in printed format).
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Genomic and microscopic dissection of large-scale chromatin compaction
Large-scale chromatin compaction varies across the human genome, and these variations correlate with differences in transcriptional activity in a limited number of model systems. However, without validated genome-wide methods directly measuring large-scale chromatin compaction, the degree to which …
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Understanding the relationship between large-scale chromatin structure and gene expression
… into a complex DNA, RNA, and protein rich chromatin fibre, creating an interdependent functional relationship between the structure of the chromatin and the activity of the genetic features stored within. The composition of active genomic regions is weighted towards transcription factors, …
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BAC transgene arrays as a model system for studying large-scale chromatin structure
The folding of interphase chromatin into large-scale chromatin structure and its spatial organization within nucleus has been suggested to have important roles in gene regulation. In this study, we created engineered chromatin regions consisting of tandem repeats of BAC transgenes, which contain …
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Targeting a Transcriptional Activator Induces Changes in Large-Scale Chromatin Structure and Nuclear Positioning
… between transcriptional activation and large-scale chromatin structure and nuclear positioning. We have used an artificial system to target large amounts of the very strong viral transcriptional activator, VP16, to specific chromosome sites. The rationale of this approach is to use this …
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Changes in large-scale chromatin structure and dynamics of BAC transgenes during transcriptional activation
Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by William Ingram (wingram2@illinois.edu) on 2010-05-14T20:52:51Z Item is restricted until 2012-05-14T20:52:43Z
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Analyzing large-scale chromatin fiber via studying cytology of DNA replication and in vivo immunogold labeling
… physical and biological methods. Although large-scale interphase chromatin has been observed repeatedly, its high-order compaction level is hard to reconcile with its active biological functions, like replication and transcription. Decondensation of such large-scale chromosome structure is …
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Creating a versatile toolkit for transgene expression using BACS and for dissecting large-scale chromatin organization
… highly compacted with histone proteins to form chromatin in interphase nuclei. Both the higher levels of chromatin folding and the spatial organization of the chromatin, referred to as large-scale chromatin organization, have been shown to correlate with transcriptional activity. One example …
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The Role of Coregulator Proteins in Nuclear Receptor Transcriptional Activity
… in the actions of ERalpha and ERbeta using large-scale chromatin arrays and ER subtype-selective ligands.
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Development of a multiscale coarse-grained chromatin model
… of the structure of biomolecules inside nano-scale chromatin. In this thesis, we develop a multiscale coarse-grained chromatin model, which integrates all-atom representations of proteins, DNA, and nucleosomes; a chemically specific coarse-grained model of kb scale chromatin; and a minimal …
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"""TSA-Seq"": a novel proximity mapping approach for studying three dimensional genome organization and function"
"The organization of chromatin and its localization in the eukaryotic cell nucleus plays a critical role in gene regulation (Agarwal and Rao, 1998; Brown, 2003; Ferrai et al., 2010) and is important for maintaining normal genome function. However, although it is known that the distribution of …
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Analyzing a calcium-dependent UV response that results in a global chromatin compaction in mammalian cells
… However, the structural change in the global chromatin in response to UV radiation and the etiology of these changes are not well known. To visualize a global chromatin structural changes upon UVB radiation, we used a fluorometric detection approach. We observed large-scale chromatin …
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Role of ORCA and ORC in chromatin organization and DNA replication
… or individual subunits of ORC including heterochromatin organization, telomere and centromere function, centrosome duplication and cytokinesis. Heterochromatic domains are enriched with repressive histone marks, including histone H3 lysine 9 methylation, written by lysine methyltransferases …
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A functional analysis of the epigenetic regulator CTCF
… for regulating transcription and altering chromatin structure. As a reporter for changes in chromatin, we employed a cell line bearing a lac operator array that can dramatically unfold its normally condensed chromatin when targeted by transcriptional proteins fused to a GFP-tagged lac …
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Regulation of SRC-3 localization and dynamics by phosphorylation during ERα-dependent transcriptional activation
… estrogen receptor alpha (ERalpha), in terms of large scale chromatin remodeling and interaction with the associated coactivator SRC-3 (Steroid Receptor Coactivator-3), a member of p160 family (28) primary coactivators. ERalpha is a steroid-dependent transcriptional factor (16) that belongs to …