Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 116 for “"lacZ"”.
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Characterisation of a conserved motif found within the Dlx cis-regulatory element URE2 and its effect on mouse URE2-lacZ activity in transgenic mice
The Distal-less-like genes, commonly referred to as Dlx genes, encode homeodomain transcription factors essential for the establishment of GABAergic interneurons in the ventral forebrain as well as their migration to the cortex. The cis-regulatory element URE2 (enhancer) is located upstream of Dlx1 …
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Complex Regulation of Pax6 Neuronal Progenitors By Rb Family Members During Corticogenesis
… our lab developed a wild type Rb promoter-LacZ transgenic reporter line (T157) that displayed dynamic and neuronal-specific expression (Agromayor et al., 2006). We generated mutant Rb promoter-LacZ transgenic lines and demonstrated that the conserved cassette controls Rb expression, …
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Regulation of the speC gene encoding ornithine decarboxylase in Escherichia coli by putrescine, spermidine and cAMP
… undefined mechanisms. A single copy of the speC’-lacZ fusion plasmid pOL-1, containing an 843 base pair fragment including the spec promoter, was transferred to the E. coli CB806 chromosome to create E. coli λCBOL. In cell-free extracts prepared from E. coli λCBOL supplemented with cAMP, …
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Lineage tracing of Ascl1-expressing cells in the maternal liver during pregnancy
… but absent in NP livers. The Rosa26floxstopLacZ/ floxstopLacZ;Ascl1CreERT2/+ mouse line was used to trace the fate of Ascl1-expressing cells during pregnancy. LacZ staining of gestation day 13 (D13) and 18 (D18) maternal livers demonstrates that D13 hepatic Ascl1-expressing cells (labeled …
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Untersuchungen zur Interaktion zwischen einer adenoviral-vermittelten Gentherapie und einer Chemotherapie mit Topotecan in etablierten humanen Ovarialkarzinom-Zelllinien
… eines therapeutischen Gens das Reportergen lacZ (beta-Galaktosidase) unter der Kontrolle des konstitutiv aktiven Promotors RSV exprimierte. Der Einfluss von Topotecan auf die Transduzierbarkeit, die aus der Transgenaktivität ermittelt wurde und die Zytotoxizität der Kombinationstherapie …
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Entwicklung von PCR-Assays und Reportergensystemen für das Studium der genetischen Variabilität und der Genregulation in Helicobacter pylori
… der Genregula-tion mit Hilfe des Reportergens lacZ ermöglichen. In den Plasmiden wurde das promotorlose lacZ-Gen mit den Antibiotikaresistenzgenen cat und km gekoppelt. Gewährleistet durch die Klonierung chromosomaler DNA-Fragmente vor das lacZ-Gen des Vektors erfolgte die Inser-tion der …
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The Intracellular Domain of EPHB1 Is Required for Axon Pathfinding at the Optic Chiasm and Corpus Callosum
… I created a new knock-in mutant mouse, EphB1 T-lacZ, that expresses an intracellular-truncated EphB1-β-gal fusion protein from the endogenous locus. As in the EphB1 -/-protein-null animals, the EphB1 T-lacZ/T-lacZ homozygotes fail to form the ipsilateral projecting subpopulation of retinal …
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Tailoring Microenvironment and Orientation of Immobilized Lactase for Improved Catalysis at Suboptimal pH
… Lactase, even exceeding that of the free LacZ-ST by 155% at pH 7, while no effect was observed for randomly immobilized LacZ. The inclusion of pH-responsive polycationic moieties in the support enabled LacZ to catalyze at pH 4, where the free enzyme is typically inactive. This study …
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Comparative Study of Colon Cancer Subclones Uncovers Potential Roles for AKAP7 and TP53RK in the Antiviral Response.
… CT26.WT and its hypersensitive subclone CT26.lacZ. This study led to the identification of retroviral insertion sites in AKAP7 and TP53RK genes, that are potentially involved in conveying sensitivity to infection by OVs and the dysregulation of the interferon antiviral response in the …
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Targeted Deletion of Functionally Validated Enhancers Defines Their Role In Mouse Limb Development
… the human enhancer sequences drive distinct <em>lacZ</em> expression patterns in limb buds of embryonic day (E) 11.5 transgenic mice. To confirm that the orthologous mouse enhancers, mouse <em>280</em> and <em>1442 </em>(<em>M280</em> and <em>M1442</em>, respectively), regulate expression in the …
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Regulating rsmA Expression in Pseudomonas aeruginosa
… aeruginosa containing a transcriptional <em>rsmA-lacZ</em> fusion to answer this question. Mutants were screened via β-galactosidase assay and transposon insertions identified via arbitrary PCR. A probable MFS transporter, we named <em>mtpX</em>, was one significant transposon mutant identified. A …
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ROLE OF CRC IN THE REGULATION OF ALGINATE IN PSEUDOMONAS AERUGINOSA
… formation and diauxic growth. When a PalgD-lacZ (translational) fusion was tested, the crc mutant showed increased algD expression as predicted for a mRNA-binding repressor. Another Ptrc-algD-lacZ (translational) construct, but missing the upstream promoter (PalgD) elements, also showed …
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Molecular studies of the sdaA and sdaB genes and their gene products in Escherichia coli K-12
… sequenced. The entire sdaA gene was fused to the lacZ gene by mutating the stop-codon of sdaA and ligating in-frame to lacZ. The fused gene directed the formation of a large protein showing both L-SD and $\beta$-galactosidase activities. L-SD#1 has been extensively purified for the first time by …
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Physical, genetic, and biochemical analyses of mannityl opine catabolism genes from the Agrobacterium Ti plasmid pTi15955
… Characterization of the transposon-induced lacZ fusion mutants by $\beta$-galactosidase activity and mannityl opine utilization patterns identified at least six genetic units associated with catabolism of these opines. Functions for catabolism of MOP and mannopinic acid (MOA) are encoded by …
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Functional Similarity of Prd-Containing Virulence Regulators In Bacillus Anthracis
… promoters fused to promoterless <em>lacZ</em> genes largely mirrored RNA-Seq data showing AtxA alone had activity on P<em>lef-lacZ</em>, and AcpA and AcpB had more activity than AtxA on P<em>capB-lacZ</em>. Studies to test the effect of AtxA levels on virulence and sporulation used …
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Molecular, Genetic and Physiological Studies on the Regulation of Conjugal Transfer of the Ti Plasmid pTiC58 by Agrobacterium Tumefaciens
… induction and autoinduction systems. Analysis of lacZ fusions determined that quorum-sensing results from TraR and AAI activating transfer gene expression only at high cell densities. The bacteria measure their density by sensing the concentration of AAI in the growth medium.
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Bibliotheken von Stationärphasen-Promotoren in gramnegativen Bakterien : Erzeugung, Screening und verfahrenstechnische Analyse
… Fluoreszenz verwendet. Vornehmlich wird das LacZ-Reportersystem primär für die Untersuchung und Charakterisierung der Expressionsprofile von Genen eingesetzt. Da bisher fast ausschließlich das LacZ-Reportersystem zur Charakterisierung von Stationärphasen-Promotoren Verwendung fand, ist dieses …
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The Regulation of Alkaline Phosphatase during the Development of Dictyostelium
… by fusing the 5' flanking sequence to the <i>lacZ</i> reporter and using an <i>in situ</i> β-galactosidase staining method. The localization of </i>lacZ</i> expression corresponds with that of the endogenous ALP enzyme suggesting that <i>alp</i> is regulated at the level of transcription. In …
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Die Expression von E-Cadherin in der Embryonalentwicklung der Maus : Analyse von cis-aktiven regulatorischen Elementen
… verwendet. <br>Verschiedene E-Cadherin-lacZ Reporter Konstrukte mit Sequenzen zwischen –6 und +16 kb inklusive aller kartierten DHS dienten der Etablierung transgener Mäuse. Die Analyse zeigte, dass Promoterfragmente ohne zusätzliche Intron Sequenzen nicht ausreichend sind, um das Gen zu …
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A Molecular Genetic Investigation of the Regulation of Outer Surface Protein C in Borrelia Burgdorferi: Identification and Characterization of the Novel Regulatory Protein BBD18
… controlling OspC expression, we adapted the lacZ reporter system from Escherichia coli for use in B. burgdorferi. Using this lacZ system and other molecular genetic approaches, we identified BBD18 as a novel factor that has the potential to negatively regulate OspC. Expression of BBD18 in B. …
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