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Showing 1 to 13 of 13 for “"lac operon"”.
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Lactose Operon Expression and Plasmid Rearrangement in Rhodopseudomonas Sphaeroides (Gram Negative Bacteria)
… initial studies expression of the transposable lac operon, Tn951, was examined. Very low levels of (beta)-galactosidase were observed in R. sphaeroides but mutants of this bacterium were recovered that were able to use lactose as the sole carbon source and which had elevated (beta)-galactosidase …
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The Expression of DNA Restriction-Modification Genes of ESCHERICHIA COLI K12
… in Escherichia coli K, hsdK::MudX (amp crop lac) operon fusion mutants have successfully been constructed and characterized for the first time. The results indicate that Promoters 1 and 2 for hsdK genes appear to be relatively weak, as reflected by about 50 units of beta-gal activity. Both …
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Regulation of Iron Sequestration Systems in Escherichia Coli and Salmonella Typhimurium (Heme, Outer Membrane)
… iron, growth temperature, and heme was examined. Operon fusions were constructed in which the lactose utilization genes were fused to the promoter of the iron-regulated gene which encodes the colicin I receptor. Regulation by iron was shown to be at the level of transcription. A regulatory mutant …
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Structure-Function Relationships of Elements of the Bacteriophage f1 Genome
… upon its own intact promoter. Gene VIII was placed under control of the E. coli lac promoter and the RNA from the new transcription unit was characterized. Coat protein was expressed from a hybrid mRNA initiated at the lac promoter. This mRNA, which contained the 5' 36 nucleotides of the lac …
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An Exploration of Fitness Landscapes: Theory and Experiments
… case study of genetic divergence in the E. coli lac operon repressor (lacI), and aim to understand the underlying processes and mechanisms that cause divergence. Divergence at this site is due to the historical contingency of mutation fitness effect on epistatic interactions with other …
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An investigation of the development and evaluation of online approaches for improved kinaesthetic learning in science
… as exemplified by three different lessons: the Lac Operon gene regulation system in E. coli, DNA transcription and translation, and Salmonella virulence factors. A mixed-methods study was conducted including three pilot studies, three randomised control trials and two sub-studies. Study …
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Signal processing and decision making in single cells
… environments regulate gene expression. The lac operon in Escherichia coli has the ability to display a bistable, "all-ornothing" response to sugar. To understand how noise drives transitions between these two stable states, we measure switching dynamics in a population of cells. A simple …
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A Stochastic Framework to Model Extrinsic Noise in Gene Regulatory Networks
… fate of lambda phage infection of bacteria, the lactose utilization system in E. coli, and a signaling network in melanoma cells. The framework captures factors that signicantly contribute to the random decision between lysis and lysogeny as well as explains the bistable switch in the model of …
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Revisiting Allostery In Lac Repressor
Lac repressor (LacI) is an allosterically regulated transcription factor which controls expression of the lac operon in bacteria. LacI consists of a DNA-binding domain (DBD) and regulatory domain (RD), connected by a linker called the “hinge”. Binding of a small molecule inducer to the RD relieves …
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Development of oxygenase biocatalysts for the production of synthetically useful cis-diol compounds
… This new strain carries the todE gene on a Lac operon regulated pMCL210 plasmid and was designated E. coli 1/210LK. After optimisation of the biotransformation, it showed an increase in the concentration of (4S,5S)-dihydroxy-3-iodocyclohex-2-en-1-one of ~40% compared to E. coli LK, thus …
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Gene regulation in baculovirus-infected insect cells : application in the development of a budded virus free expression system
… employing components of the Escherichia coli lac operon to block expression of essential genes required for BV production during VLP/protein production (the LacI/O Repressor System). Initially, RNA interference was used in a screening process to inhibit expression of a large number of …
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Stochastic and physical modeling of fundamental biological processes
… in detail two systems: an improved model of the lac genetic switch which includes DNA looping in its gene regulation mechanism, and a spatially resolved, whole-cell model of ribosome biogenesis in Escherichia coli, which we then extend to include cell growth and replication of its genome. For the …
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Computational investigations of evolutionary transitions during development of the cellular translation and transcription machinery
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