Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 15 of 15 for “"laboratory evolution"”.
-
Laboratory evolution and natural transformation in Acinetobacter baylyi ADP1
… biotechnological use however is inhibited by the evolutionary instability of the competence, the large homology requirement for the efficient transformation of DNA into the ADP1 genome, and incomplete knowledge regarding the composition and regulation of the protein complex involved in DNA uptake. …
-
Adaptive Laboratory Evolution of Scenedesmus obliquus for Increased Carbohydrate Content and Biomass Productivity
… productivity. This thesis explores two adaptive laboratory evolution strategies for improving <em>Scenedesmus obliquus</em>: single UV-mutagenesis and low light cultivation at a high dilution rate to produce cultigens with greater carbohydrate content and iterative UV-mutagenesis and selection …
-
Metabolic engineering and adaptive laboratory evolution of yeasts to achieve efficient microbial cell factory
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-05-01
-
Enhance heme biosynthesis in Saccharomyces cerevisiae
… in Saccharomyces cerevisiae through Adaptive Laboratory Evolution (ALE) and Atmospheric and Room Temperature Plasma (ARTP) mutagenesis, complemented by strategic nutrient supplementation. Despite the theoretical promise of ALE and ARTP to induce beneficial mutations for augmented heme …
-
Phenotypic Switching of Bacterial Cells in Extreme Environments
… extremes, and sets a foundation for long-term laboratory evolution of a mesophilic bacterium to the extremes of environmental conditions. Furthermore, the results presented here are also useful in assessing the kind of microbial lifeforms that may exist elsewhere in our solar system, such as …
-
Cooperative assembly confers regulatory specificity and promotes evolution
… a simple, generalizable means for building evolutionarily robust gene circuits that can be scaled to a wide range of host organisms and applications. Assemblies of cooperative TRs have also been found to facilitate the gene regulatory network rewiring that drives evolution. TRs that arise in …
-
Synthesis, Characterisation and Evolution of Escherichia coli with a Compressed Genetic Code
… and its further development. Multiple rounds of laboratory evolution and deletion of the decoding elements for the removed codons (tRNAs and release factor 1) yielded a cell with a compressed genetic code and improved growth, to advance genetic code expansion. Finally, in Chapter IV the powerful …
-
Improving Bacterial Therapeutics and Diagnostics through Synthetic Biology
… that reduce EcN viability. We performed adaptive laboratory evolution on EcN to select for mutated populations with greater tolerance to pH and bile and we identified specific mutations that contribute to their enhanced viability in the presence of these chemical stressors. The developed strains …
-
Engineering yeast strains for producing fuels and value-added chemicals from cellulosic biomass
… cassettes were massively amplified through laboratory evolution, resulting in significantly improved cellobiose fermentation. Besides ethanol production, the simultaneous co-conversion of cellobiose and xylose strategy was successfully applied to improve the production of value-added …
-
A Systematic Method for Enhanced Expression of Alcohol Dehydrogenases in Escherichia coli
… to the E. coli strains of interest via adaptive laboratory evolution; the final evolved strain provided a further 4.15-fold and 4.87-fold improvement to the soluble yield of the system for the two alcohol dehydrogenase models. The evolved expression strain, E. coli ALE strain ‘D9’, showed key …
-
Engineering protein components for living electronics
Living cells sense and respond to an astounding array of different molecules. Integrating these cells into digital devices to produce living electronics has the potential to create useful devices, such as bioelectronic sensors that combine the sensitivity and specificity of biological systems with …
-
Exploring protein fitness landscapes with new high-throughput technologies
… tool to visualize core principles of protein evolution. Acquiring a new function, for example the laboratory evolution of an enzyme to convert an industrially relevant substrate, can be understood as a stepwise climb through a fitness landscape, reaching higher fitness (or activity) with each …
-
Proteome, transcriptome and metabolome plasticity in closely related strains of Escherichia coli - K12 and its diversity during molecular evolution processes
… E. coli is frequently used in molecular evolutionary studies. A few potential reasons for this are: its capacity to propagate and reproduce quickly, facilitating the evolution experiments for many generations in a short time span, and capability to store the evolved and ancestor strains, …
-
Metabolic engineering of yeast strains for renewable biomass utilization and valuable chemical production
… with CDT-2 and CBP were very inefficient, laboratory evolution approach was employed to enhance cellobiose utilization. I isolated an evolved strain that harbored one SNP change in CDT-2 that is responsible for enhanced cellobiose utilization. With evolved CDT-2 and CBP, cellobiose …
-
Electrogenetic pathways in cyanobacteria
… unknown. This limits our understanding of the evolutionary history, biochemical mechanisms and physiological functions of electrochemical signal transduction in oxygen-evolving photosynthetic bacteria, hindering the development of optimised, solar-powered biotechnologies. Aiming to address …