Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 33 for “"knockout mutants"”.
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Characterisation of the AT4G11100 gene, a negative regulator of disease resistance in Arabidopsis thaliana
… 8 known genes in the region. Analysis of T-DNA knockouts of these 8 genes suggests that AT4G11100 is the mostly likely candidate for CIR1. This project established that the disease resistance phenotype of cir1 is temperature dependent and linked to reduced plant growth. Genetic crosses between …
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Characterization of PRD-1 Mutation in Neurospora crassa
… interval, candidate genes were sequenced, and knockout mutants were screened in this interval for prd-1 phenotype. A candidate gene was found to have a mutation that affects the splicing of the mRNA. The identity of the gene was confirmed by complementing the prd-1 mutant with a wild type copy …
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Development of a Transposon Based Activation Tagged Mutant Population in Tomato for Functional Genomic Analysis
… to produce activation tagged and knockout mutants in the processing tomato variety, M82. The construct contained hygromycin resistance (hyg), green fluorescent protein (GFP), and maize transposase (TPase) on the stable Ac element, along with a 35S enhancer tetramer and glufosinate …
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Modulated Functions of The Fanconi Anemia Core Complex
… FANCD2 ubiquitination, we established isogenic knockout mutants deficient in FA core genes and determined the loss-of-function effects on the activation of FA pathway and on cellular survival against ICLs. Our results suggest three potential functional modules in the FA core complex: …
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Seed germination, kanamycin sulfate selection, and the influence of nitrogen treatments on an insertional mutant population of Fragaria vesca
… methods of generating unique Ac/Ds insertional mutants in a population of Fragaria vesca, various methods of seed germination, kanamycin screening, and the effects of varying nitrogen fertilization on diploid strawberry have been examined. Seed germination was improved to 42% in B5 liquid medium …
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The role of SCLA-type GRAS transcriptional regulators in plant development and stress responses
… reproductive organs. I have produced CRISPR knockout mutants in *Marchantia polymorpha* and its AM-compatible sister species, *Marchantia paleacea*, in order to investigate the functions of SCLA in liverworts. Phenotyping revealed Mp*GRAS7* to be involved in the vegetative to generative …
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Cornichon Proteins: Unexpected Roles in Plant Pathogen Infection, ER Morphology Maintenance and Pollen Development
… Erv14. Taking advantage of Arabidopsis thaliana knockout mutants and knockdown of gene expression in Nicotiana benthamina, I also discover that CNIHs unexpectedly play crucial roles in pollen development, infection of a bacterial pathogen, and maintenance of ER tubules. I further confirm that CNI …
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Developmentally regulated SUMOylation in the ciliate Tetrahymena thermophila
… for SUMOylation during conjugation. Germ-line knockout mutants of <em>SUMO (SMT3)</em> and <em>UBA2 </em>are vegetative lethal and conditional mutants dependent on a cadmium-inducible metallothionein promoter exhibit reduced cell growth and increased sensitivity to DNA damaging agents upon …
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Studies on cathepsin B of Eimeria tenella and pyroglutamyl peptidase of Leishmania major
… enzyme localised to the parasite cytoplasm. PPI knockout mutants were generated, and knock-out of the PPI activity did not seem to induce a phenotype in L. major. The parasites retained the properties in vivo and in vitro of L. major wild type cells. The over-expression of the active PPI in L. …
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Regulation of (1,3;1,4)-β-Glucan Synthesis in Barley (<i>Hordeum vulgare</i> L.)
… the functional characterization of CRISPR/Cas9 knockout mutants of putative (1,3;1,4)-β-glucan synthases revealed that HvCslF9 is not essential for (1,3;1,4)-β-glucan synthesis in the grain, whereas HvCslF6 disruption led to decreased (1,3;1,4)-β-glucan content and altered grain size and shape. …
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Towards functional assignment of Plasmodium membrane transport proteins: an experimental genetics study on four diverse proteins
… expression and phenotypical analysis of knockout mutants. We employed the murine malaria model parasite Plasmodium berghei and in vitro blood cultures of the human malaria parasite Plasmodium falciparum. For this study, we selected one conserved MTP called FT2, which was previously shown …
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Towards functional assignment of Plasmodium membrane transport proteins: an experimental genetics study on four diverse proteins
… expression and phenotypical analysis of knockout mutants. We employed the murine malaria model parasite Plasmodium berghei and in vitro blood cultures of the human malaria parasite Plasmodium falciparum. For this study, we selected one conserved MTP called FT2, which was previously shown …
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The role of the histone variant H3.3 and its chaperones in the response to DNA damage
… genome editing, I created H3.3, ATRX and HIRA knockout mutants and investigated their response to UV irradiation. h3.3 TK6 cells are not hypersensitive to acute DNA damage but exhibit persistence of DNA damage markers. h3.3 cells also exhibit a change in their cell cycle distribution after UV …
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Development and application of a general method for somatic cell genetics
… system. In this approach, libraries of knockout mutants can be generated in any cultured cell line using large-scale lentiviral single-guide RNA (sgRNA) pools and screened under both positive and negative selection. After the initial, proof-of-principle experiments, I constructed a …
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The roles of papain-like protease-related proteins in viral replication and host immunity
… effects. The infection of nsp2TF/nsp2N knockout mutants significantly upregulated antiviral innate immune responses in vitro. Furthermore, manipulating the expression of nsp2TF/nsp2N could enhance innate and adaptive immunity in pigs, providing potential basis for modified live vaccine …
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Functional specialization of Arabidopsis poly(A) polymerases in relation to flowering time and stress
… the detailed analysis of Arabidopsis paps1 mutants revealed a subset of genes that is preferentially polyadenylated by the cPAP isoform PAPS1 (Vi et al. 2013). Thus, the specialization of cPAPs might allow the regulation of different sets of genes in order to optimally face developmental or …
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Identification of growth-related tonoplast proteins in Arabidopsis thaliana
… genes in leaf growth on a macroscopic scale, knockout mutants of the candidate growth related genes were screened for growth phenotypes. To this end, a system for non-invasive automated leaf growth phenotyping was established, based on a commercially available image capture and analysis …
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Generation of midbrain dopaminergic neurons in vivo and in vitro: the role of Neurogenin2
… Furthermore, when I analysed the Ngn2 knockout mutants, I found that they displayed an early loss of mesDA neurons that was partially maintained at postnatal stages, showing that Ngn2 has a role in the generation of the mesDA neurons. No other neuronal subtype in the VM was affected …
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The role of genomic islands in virulence of Pectobacterium carotovorum subspecies brasiliensis on potatoes
… of P. carotovorum subsp. brasiliensis ICMP19477, knockout mutants were constructed by allelic exchange mutagenesis. Mutations in the non-ribosomal peptide synthetase cluster of P. carotovorum subsp. brasiliensis (on PbN1_GI24) did not result in significant differences in virulence of the wild type …
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Investigating CDK9 inhibitor treatment during the innate inflammatory and regenerative response in a zebrafish model of cardiac injury
… I developed a selectivity assay using cdk9-/- knockout mutants that demonstrated AT7519 is a more selective CDK9 inhibitor than FVP. These findings highlight the potential of AT7519 as a promising treatment that resolves neutrophilic inflammation following cardiac injury and promotes …
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