Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 45 for “"knockout mouse model"”.
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Functional Study of Hemogen Knockout Mouse Model
<p>Mouse Hemogen (Hemgn) is regarded as a homologue of human Erythroid Differentiation Associated Gene (EDAG). EDAG overexpression has been postulated for association with some leukemia cases. Meanwhile, Hemgn has been found to contribute to Hoxb4 mediated hematopoietic stem cell expansion. Based …
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Generation of focal Depdc5 knockout mouse model and implications for focal epilepsy
… virus (AAV) mediated focal cortical Depdc5 knockout mouse model. Viral vectors containing either 2/8AAV-Cre or control 2/8AAV-GFP were injected into the unilateral motor cortex of postnatal day zero or day one Depdc5 floxed (Depdc5c/c or Depdc5c/-) mouse pups. We confirmed a significant …
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Defining the Role of Pannexin1 in Skeletal Muscle and Satellite Cell Function in vivo and ex vivo Using a Novel Conditional Knockout Mouse Model
… Using a novel skeletal-muscle-specific Panx1 knockout (Panx1^smKO) mouse model, this thesis highlights the influences Panx1 plays in muscular development and maintenance, as well as in muscle function. Loss of Panx1 led to a fiber-type switch coinciding with smaller fibers in both the tibialis …
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The effects of leptin on placental development and function, and offspring behavior in mice
… the importance of the leptin receptors of the mouse conceptus in placental development and function. We utilized a leptin receptor knockout mouse model, and found significant alterations in gene expression in placentas lacking the receptor, suggesting that placental function may be altered. The …
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Investigating The Role of The P63 Isoform Δnp63 In Lung Stem Cell Populations and Lung Cancer
… We utilized a ΔNp63-specific conditional knockout mouse model to determine ΔNp63’s role in lung progenitor cells and lung cancer. In vivo and in vitro experiments revealed a role for ΔNp63 in maintaining lung progenitor cells. ChIP-seq results indicate that deletion of ΔNp63 results in …
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Molecular biology of peripheral nerve regeneration: Expression and role of Netrin-1, DCC and Runx2
… repair. Using a Schwann cell-specific Netrin-1 knockout mouse model, I showed an increased disorganisation and misdirection of re-growing axons within the nerve bridge following transection injury. However, this did not affect the number of axons innervating the distal nerve. Moreover, …
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MULTI-ORGAN FAILURE DRIVEN BY TELOMERE UNCAPPING
… In this work, we adopted an inducible Trf2 knockout mouse model to investigate the consequences of telomere uncapping at the organismal level. Trf2 deletion was rapidly achieved across multiple tissues and this event was accompanied by DDR activation and multi-organ functional failure, …
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The Role of Toll Like Receptor-4 in Exercise-induced Myokine Response and Regulation of Skeletal Muscle Metabolic Adaptation
… we developed a novel muscle-specific TLR4 knockout (mTLR4-/-) mouse model on C57BL/6JJ background. The differential inflammatory and metabolic responses between mTLR4-/- mice and wild type (WT) littermates were examined following exposure to either exercise or muscle stimulus. Accordingly, …
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BDNF and Astrocyte TrkB.T1 Signaling as a Mechanism Underlying Astrocyte Synapse Interactions in Motor and Barrel Cortex
… Using an astrocyte-specific conditional TrkB.T1 knockout mouse model, we demonstrate that deletion of TrkB.T1 results in hyperactive locomotion, with increased voluntary running and perseverative motor behaviors. Through a combination of molecular and cellular approaches, we demonstrate that the …
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Matriptase/pdgf D/beta-Pdgfr Signaling Axis In Human Prostate Cancer: The Role Of Pten In The Regulation Of Pdgf D Expression
… PTEN (phosphatase and tensin homolog) knockout mouse model, we found an increase in PDGF D expression and ?-PDGFR phosphorylation upon loss of PTEN. Upon inhibition of the PI3K pathway, PDGF D/ ?-PDGFR induction was abolished in PTEN-/- cells. Among Akt isoforms, downstream effectors of …
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Deciphering The Roles of Δnp63 In Regulating Epithelial to Mesenchymal Transition, Cancer Progression and Metastasis
… Utilizing a <em>ΔNp6</em>3-conditional knockout mouse model generated in the Flores lab, I demonstrated that the loss of ∆Np63 in basal epidermal cells elicits an “irreversible” epithelial to mesenchymal transition (EMT) via activation of EMT-associated transcription factors, leading to …
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A study of the beneficial role of thiol antioxidant supplementation in various forms of glutathione deficiency
… of glutathione depletion in two different models of oxidative stress. The first model represented oxidative stress processes in age-related macular degeneration. This part of the study focused on the protective effects of NACA on t-butyl hydroperoxide (tBHP) induced oxidative damage in …
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Silencing Endothelial EphA4 Alters Transcriptional Regulation of Angiogenic Factors to Promote Vessel Recovery Following TBI
… herein utilizes an endothelial-specific EphA4 knockout mouse model (KO-EphA4flf/Tie2-Cre) to determine the extent to which this receptor may influence vascular regrowth following TBI. Analysis of the colocalization and proximity of endothelial and mural cell markers (i.e. PECAM-1 and PDGFRβ, …
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Separate Roles of FAN1 and Fanconi Anemia Proteins in DNA Interstrand Crosslink Repair and Human Disease
… implicated in ICL repair. Furthermore, the Fan1 knockout mouse model recapitulates many of the phenotypes observed in human KIN patients, including renal karyomegaly and liver dysfunction. Fan1-deficient mice displayed hypersensitivity to ICL-inducing chemotherapeutic drugs and developed bone …
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Consequences of Endogenous Mitochondrial Oxidative Stress in the Heart
… controls. In this study, the constitutive Sod2 knockout mouse model was used. All mice were treated daily with the SOD2 mimetic, Euk-189. For the first time, echocardiography was performed on constitutive Sod2 nullizygous mice. Sod2 mice were shown to have impaired systolic function, as …
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The Role of MicroRNAs in Craniofacial Development
… To test this hypothesis a conditional Dicer knockout mouse model was used. In this model, floxed Dicer alleles were knocked out in the first pharyngeal arch precursor tissues of the palate. Embryos lacking Dicer in their palatal precursors experienced craniofacial developmental defects that …
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Analysis of the Role of the Transcription Factor C/EBPbeta in Controlling Uterine Functions During Early Pregnancy
… steroid hormone signaling, endometrial tissue remodeling and maternal-fetal communications. A successful pregnancy is the outcome of the timely integration of these events during the early stages of implantation. The involvement of ovarian steroid hormones, estrogen (E) and progesterone (P), …
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Analysis of the role of the transcription factor C/EBPβ in controlling uterine functions during early pregnancy
… steroid hormone signaling, endometrial tissue remodeling and maternal- fetal communications. A successful pregnancy is the outcome of the timely integration of these events during the early stages of implantation. The involvement of ovarian steroid hormones, estrogen (E) and progesterone (P), …
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Novel Roles of the Aryl Hydrocarbon Receptor in Normal Physiology and Epigenetic Reprogramming
… a lethal wasting syndrome. In recent years, AhR knockout and antagonism have been reported to confer resistance to western diet induced obesity as well as reversing diet induced obesity. Together, these observations suggest a direct role for AhR regulating normal metabolic homeostasis and …
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Using molecular approaches to understand Complex I deficiency in mouse models
… complex I dysfunctions remain unclear. Here, two mouse models of complex I-linked mitochondrial disease were analysed structurally by cryo-EM to understand the mechanisms of their pathogenesis. The first part of this thesis explores complex I from the ND6-P25 mouse model, which contains a …
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