Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 1381 for “"knockout"”.
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Functional Characterization of CRIP1a Knockout Mice
… of CRIP1a by characterizing the first CRIP1a knockout (KO) mouse line. The absence of CRIP1a was confirmed in KO mice using quantitative PCR and immunoblotting. I hypothesized that CRIP1a KO mice would exhibit enhanced CB1 receptor-mediated G-protein activity in the CNS, as well as …
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Functional Study of Hemogen Knockout Mouse Model
… on these postulations and evidences, a Hemgn knockout mouse model has been generated to study its function in normal and stress hematopoiesis. I confirmed the Hemgn expression in hematopoietic organs including bone marrow and spleen, as well as round spematids in testis. Hemgn is expressed in …
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Characterization of Inducible SMC-specific Drosha Knockout Mice
… A transgenic animal model to study SMC-specific knockout of Dicer, one of two RNase III proteins in miRNA biogenesis, showed that contractile motility in the mutant intestine was dramatically decreased (Park, Yan, et al., 2011). The purpose of this study was to investigate the function of Drosha, …
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Generation and characterization of spred-2 knockout mice
… organism was still unclear. Therefore, Spred-2 knockout mice were generated using a gene trap approach. The Spred-2 deficiency was verified on RNA and protein levels and the lack of functional Spred-2 protein in mice caused a dwarf phenotype similar to achondroplasia, the most common form of …
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Learning and cemory in the cav1.2 knockout mouse
… of Morris water maze experiments were performed. Knockout mice showed no deficits in short-term (24-hr) memory trials; however, on a 30-day memory probe, knockout mice performed significantly more poorly than their littermate controls. These results indicate the importance of forebrainspecific …
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Neurochemische und autoradiographische Untersuchungen von Serotonin-Transporter-Knockout-Mäusen
… zu untersuchen, wurde eine transgene 5-HTT-Knockout-Maus entwickelt, die als Grundlage der Untersuchungen der vorliegenden Arbeit diente. Vor allem aufgrund der Assoziation des kurzen Allels des 5-HTT-Promotorpolymorphismus mit M. Alzheimer wurde die Untersuchung der Mäusegehirne im Hinblick …
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Enhanced methylglyoxal formation in cystathionine γ-lyase knockout mice
… manner in A-10 cells. In 6-22 week-old CSE knockout male mice (CSE⁻/⁻), mice with lower levels of vascular H₂S, we observed a significant elevation in MG levels in both plasma and renal extracts. Renal triosephosphates were also significantly increased in the 6-22 week-old CSE⁻/⁻ mice. To …
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Nicotine Sensitization in β-Arrestin 2 Knockout Adolescent Mice.
<p>ß arrestin-2 is a protein involved in signaling of D2 receptors and plays a mediating role in sensitization to psychostimulants and the opiate morphine. In this study, 3-4 week old BA-2 KO and wild type C57/B6 mice received nicotine tartarate (s.c, 0.5 mg/kg free base) for 7 or 14 consecutive …
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THE RESPIRATORY RESPONSE OF TRPV1 KNOCKOUT MICE TO TRIGEMINAL IRRITANTS
… (C57Bl/6J) mice was compared to female Trpv1 knockout mice for a variety of compounds in an attempt to determine if TRPV1 is responsible for the detection of the irritants. Trpv1 knockout mice did not appear to show respiratory rate depression when exposed to cyclohexanone, a known TRPV1 …
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Cardiovascular Characterization of Cx40/Panx1 Single and Double Knockout Mice
… possibility we crossed Panx1 null mice with Cx40 knockout mice and characterized the first global connexin/pannexin double knockout mouse. Intriguingly, the combined ablation of both Cx40 and Panx1 caused decreased prenatal and newborn survival, but did not affect the fertility or lifespan of …
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The IN4MER CRISPR/Cas12a Multiplex Knockout Platform and Its Applications
… a couple of studies have conducted dual-gene knockout experiments on paralog pairs using different approaches to identify synthetic lethal interactions. In this study, we conducted a meta-analysis of CRISPR genetic interaction screens. We identified a candidate set of synthetic lethals that …
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Mechanisms of Hypoxia-Induced Neurovascular Remodeling in PlGF Knockout Mice
… growth factor (PlGF) is unknown. Using PlGF knockout (PlGF-/-) mice exposed to whole body hypoxia (10% oxygen) for 7, 14 and 21-days, we show that PlGF-/- animals exhibit a delay in the angiogenic response of the brain to hypoxia. PlGF-/- microvessels had a significant increase in fibrinogen …
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CRISPR/Cas9-mediated ATG7 knockout results in enhanced osteosarcoma chemosensitivity
… In the current study, CRISPR/Cas9 was used to knockout (KO) ATG7, a key gene in the autophagy process, in HOS-143B (highly metastatic/aggressive) osteosarcoma cells. Successful knockout of ATG7, and the autophagy process, was validated by qPCR and cell viability assays. Furthermore, a …
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Developmental Deregulation and Tumorigenesis Inhibition In 14-3-3Zeta Knockout Mouse
… were differentially expressed in the 14-3-3z knockout tumors. MiR-126 was an important microRNA mediating both the lethality and tumorigenesis inhibition phenotype.</p> <p>In addition, 14-3-3z knockout mice are smaller than their litter mates. This growth retardation was accompanied with …
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Biases and Blind-Spots In Genome-Wide Crispr-Cas9 Knockout Screens
… <p>Studies showed that CRISPR monogenic knockout screens can identify specific processes that cells rely on for growth and proliferation, which is a crucial step in identifying candidate cancer-specific therapeutic targets. While it is widely accepted that CRISPR screening is both more …
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Synaptic Plasticity in the Cerebral Cortex of Fragile X Knockout Mice
… of the visual cortex was examined in young Fmr1 knockout and wildtype mice. The rate of developmental synaptogenesis between the two genotypes was similar, but Fmr1 knockout mice exhibited levels of synaptic protein synthesis lower than those of wildtype mice. The role of FMRP in …
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