Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 1012 for “"knockdown"”.
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RNA Knockdown of the Immune Checkpoint VISTA Promotes Tumor Regression
… we used Antisense oligonucleotides (ASOs) to knockdown VISTA expression on myeloid cells. ASOs are synthetically generated single stranded oligonucleotides that alter messenger RNA to either diminish, recover, or adapt protein expression. We hypothesize that reducing VISTA expression on …
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Etablierung und Charakterisierung einer Tetracyclin-induzierbaren PHD2-Knockdown-HeLa-Zelllinie
… wurden Tetracyclin-induzierbare PHD2- und PHD3-Knockdown-Zelllinien etabliert und die Bedingungen für einen möglichst effizienten Knockdown optimiert. Weiterführend wurden die Zelllinien hinsichtlich ihrer Expression von PHD1-3 sowie HIF-1α und dessen Zielgen VEGF (vascular endothelial growth …
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Effects of benzo[a]pyrene and CYP19a1b knockdown on zebrafish development
… a transient morpholino (MO)-mediated CYP19a1b knockdown or exposure to an aromatase inhibitor (fadrozole) during early development. One-cell zebrafish embryos were injected with a CYP19a1b-MO or control-MO. Other non-injected embryos were exposed to nominal waterborne concentrations of BaP (0, …
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Knockdown of nfa1 Gene Cloned from Naegleria fowleri by Antisense RNA
… of proteins cloned from N. fowleri, the gene-knockdown analysis by a transfection system are not yet established. In this present study, to describe the association of an Nfa1 protein in vitro cytotoxicity of N. fowleri to target cells, an antisense RNA or siRNA of nfa1 gene were transfected …
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Sustained hydrogel-based delivery of RNA interference nanocomplexes for gene knockdown
… system for targeted and sustained gene knockdown both in vitro and in vivo using dendrimer and lipid based nanoparticles in combination with synthetic polyethylene glycol (PEG) and natural fibrin hydrogel scaffolds. Four siRNA nanocarriers were investigated for siRNA delivery, that is, …
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Auswirkungen des LRRK2-Knockdown durch RNA-Interferenz auf die murine dopaminerge Zelllinie MN9D
Mutationen im Protein LRRK2 wurden im Zusammenhang mit klinischen Symptomen beschrieben, die dem Idiopathischen Parkinsonsyndrom (IPS) nahezu gleichen. So findet sich neben vielen anderen Mutationen die häufigste pathogene Mutation für das IPS im LRRK2-Gen. Die Aufklärung der molekularbiologischen …
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Modeling 3D Retinogenesis in Mouse Embryonic Stem Cells Following CRISPR-mediated Crx Knockdown
… we utilized the CRISPR method as a means to knockdown the transcription factor CRX, which is known to regulate key developmental decisions during retinogenesis, and as such, mutations in CRX have resulted in various diseases such as Leber Congenital Amaurosis, Cone-Rod Dystrophy, and …
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Partial GSK3 knockdown improves muscle performance in mdx mice: investigating the cellular mechanisms
… thesis. To this end, male muscle-specific GSK3 knockdown mice (C57BL/6J background) were bred with female homozygous D2 mdx mice to produce 1) mdx mice with partial (30-40%) muscle-specific GSK3 knockdown (mdx/GSK3KD) and 2) mdx mice with GSK3 intact (flox control). To determine how GSK3 …
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In Vivo Metabolic and Vascular Response to Hypoxia in Twist Knockdown Murine Breast Cancer
<p>Twist transcription factor is often overexpressed in aggressive tumors. Although needed in early embryonic development for organogenesis, Twist is known to induce an epithelial to mesenchymal transition in cells. In cancer, epithelial to mesenchymal transitions can lead to increased motility and …
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Autophagy and EVs in cancer cells. Effect of ATG8 knockdown on the biogenesis of CD81+ extracellular vesicles.
Dysregulation of both the autophagy pathway and the biogenesis of extracellular vesicles (EVs) in cells is involved in cancer development. Evidence is now emerging that these pathways may somehow be interlinked via ATG proteins. Further elucidation of the relationship between these pathways will …
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Knockdown of Heparan Sulfate Via NDST1 knockout Does Not Inhibit Mouse Aortic Endothelial Cell Alignment In-Vivo
… sulfation. NDST1 KO animals had a significant knockdown of NDST1 sulfation and a normal expression of HS3ST1 sulfation confirming the presence of the HS backbone. This result supported the conclusion that the HS backbone remains intact and functional despite the deletion of NDST1 sulfation.</p>
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COP9 signalosome subunit knockdown in K562 provides novel insight into the function and potential regulation of the CSN
… and chronic myeloid leukemia model, K562. Both knockdowns had similar consequences for CRL activity whilst having divergent effects on the levels of SRS mRNA. Knockdown of either subunit also resulted in a common sequential proteasome-dependent loss of SRS proteins, an observation that had not …
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The Roles of Krüppel-Like Factor 1 (KLF1) in the Human Fetal Erythroid Compartment.
… β-globin and Bcl11A gene expression using KLF1 knockdown in in vitro-differentiated CD34+ human umbilical cord blood cells. -globin expression appears dependent on KLF1; it is increased with modest KLF1 knockdown but not in cells with low KLF1. KLF2 mRNA amounts are usually increased in KLF1 …
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Exploring the specificity and mechanisms of siRNA-mediated gene silencing in mammalian cells
… interfering RNAs (siRNAs) are routinely used to knockdown gene expression. siRNAs bind to their target sequence and guide transcript cleavage and subsequent degradation. This type of silencing is associated with equivalent levels of RNA and protein knockdown. siRNA-mediated knockdown was …
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Functional analysis of Smyd1 and Myomesin in sarcomere organization in zebrafish embryos
… in both skeletal and cardiac muscles. Knockdown of smyd1 results in complete disruption of sarcomere organization. The molecular mechanism by which Smyd1 controls myofibril assembly is not clear. In this study, we analyzed the sub-cellular localization of Smyd1, the effect of smyd1 …
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Effects of Regional Deletion of Rab3A-interacting Molecule and PTEN on Brain Function
… describes experiments designed to delete/knockdown molecules from targeted neuronal populations to study brain region-specific behavioral functions. To this end, I utilized two different conditional knockdown techniques to study the role of the presynaptic active zone molecule …
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Non-viral drug delivery systems for immune modulation
… formulations for siRNA delivery and demonstrated knockdown of 25%. Confocal imaging showed that polymer coating increased localization of the nanoparticles to the cell membrane, endosomes and nucleus. Polycation surface-modification seemed broadly extendable to a biodegradable polymer particle …
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Context Dependent Roles of MDMX (MDM4) and MDM2 in Breast Cancer Proliferation and Circulating Tumor Cells
… mouse model and demonstrated that MDM2 knockdown considerably attenuated primary tumor growth. However depletion of MDM2 did not reduce tumor invasiveness and failed to increase E-Cadherin levels<em>.</em> Conversely in mtp53 (R280K) expressing MDA-MB-231 cells, which belong to …
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