Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 214 for “"knock-down"”.
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THE EFFECTS OF SYNAPSIN II KNOCK-DOWN IN THE RAT MEDIAL PREFRONTAL CORTEX ON ATTENTION
… the medial prefrontal cortex (mPFC) synapsin-II knockdown (KD) model. Prior to this study, the cognitive effects of mPFC synapsin-II KD had yet to be reported. Using a 14-day continuous infusion of antisense (AS) deoxyoligonucleotides for synapsin-II to the mPFC, the results of the KD model have …
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Development of a SCA7 patient-derived lymphoblast cell model for testing RNAi knock-down of the disease-causing gene
… interference (RNAi) based therapy, in order to knock down the expression of the mutant transcript in heterozygous patients. Although this approach has been tested in an artificial cellbased model of SCA7, focus has shifted towards testing the therapy in SCA7 patient-derived transformed …
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Signalling and function of the small Rho GTPase RhoJ in endothelial cells
… is an endothelial expressed Rho GTPase, and its knock-down impairs endothelial cell (EC) migration and tubulogenesis, increases stress fibre (SF) and focal adhesion (FA) numbers. This work aimed to determine the intracellular localisation of RhoJ, identify its binding partners, test how it is …
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Amphiregulin (areg) And Epidermal Growth Factor (egf): Disparate In Egfr Signaling And Trafficking
… In the presented dissertation we successfully knocked-down AREG expression in SUM-149 cells by lenti-viral infection of AREG shRNA. In the absence of AREG expression, SUM-149 cell growth was slowed, but not completely inhibited. Furthermore, cells infected with AREG shRNA constructs showed an …
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Adherens Junctions and The Actomyosin Network Regulate Organ Growth By Modulating Hippo Pathway Activity In Drosophila
… module members deregulate Yorkie activity, the downstream transcriptional effector of the Hippo pathway. Importantly, it is unclear if AJ and basolateral components act through the same or parallel mechanisms to regulate Yorkie activity.</p> <p>Here, we dissect how loss of AJ and basolateral …
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Nuclear receptor co-repressor functions in prostate cancer; in vitro, in vivo and in silico approaches
… of NCOR1, via the HDAC inhibitor SAHA, or NCOR1 knock-down, via shRNA, restored PC-3 cells sensitivity to NR agonists with exception of Vitamin D and Thyroid Hormone T3. NCOR1-knock down led also to a re-expression of basally repressed genes, as measured via Microfluidic Gene-Card analysis …
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Regulation of Actin Dynamics During Drosophila Germband Extension
… for appropriate biphasic interface contraction. Knock down of two F-actin regulating proteins, Dpod1 and coronin, results in aberrant interface dynamics and severe disruption of germband extension. Close examination of interface and F-actin aggregate dynamics in coronin knock down reveals that …
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Investigating the coordination of germline stem cell fate by ecdysone signaling and BMP signaling in the Drosophila ovary
… Over-expression of Ecdysone Receptor (EcR), knock-down of bag of marbles (bam), or over-expression of constitutively active thickveins (tkv) in all germ cells results in germ cell tumors at similar stages of differentiation. This suggests that ecdysone signaling and BMP signaling converge on …
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Funktionelle Analyse der Interaktion und Lokalisation von Replikationsfaktoren und replikationsrelevanten Proteinen in Mauszellen
… demonstriert werden. Ein siRNA-vermittelter Knock Down von HP1a zeigte jedoch keinen direkten Einfluß auf die Replikation. Es konnte hingegen gezeigt werden, daß ein Knock Down von HP1a in signifikatnen Defekten in der Cytokinese und einer deutlich verlangsamten Zellproliferation resultiert. …
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Enhancing the production of Omega-3 polyunsaturated fatty acids in marine diatoms
… droplet phenotype were also observed. Targeted knock-down of native genes involved in the omega-3 biosynthetic pathway was carried out in T. pseudonana to further understand endogenous omega-3 fatty acid production. Cells targeted for the knock-down of Δ9-desaturase exhibited a drastically …
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Engineering mammalian cell line to improve sialylation
… is utilized as a genetic approach to knock down the activity of sialidase which is responsible for cleaving terminal sialic acid. At the first stage of the studies, 21-nt double stranded siRNA sequences capable of knocking down sialidase are identified. The best sialidase siRNA …
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The Role of Dax-1 in Regulating Pluripotency in mouse Embryonic Stem Cells
… Dax-1 gene in mouse ES cells found that upon the knock down of Dax-1, ES cells differentiated. These findings indicate that Dax-1 functions in a novel role in the maintenance of a relatively undifferentiated state in ES cells.</p> <p>Dax-1 is important in early embryonic development and, when …
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조골세포 사멸 및 분화에서 GSK3b의 역할
… lithium, a pharmacological antagonist, or gene knock-down by siRNA prevented the Dex-induced apoptosis. Unexpectedly, Dex also activated p38 mitogen-activated protein kinase (p38 MAPK); however, the inhibition of p38 MAPK further increased apoptosis in Dex-treated osteoblasts. These results …
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Funktionelle Charakterisierung und ultrastrukturelle Lokalisierung der Isotypen des Hüllprotein-Komplexes Coatomer
… induzierbaren, individuellen g1- oder g2-COP knock-down erlaubten. Der knock-down der einzelnen g-COP Isoformen konnte, wie auch ein RNAi-basierter knock-down beider Isoformen gleichzeitig, nur transient erreicht werden. Dies unterstützt eine essentielle und unterschiedliche Beteiligung von …
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The role of the mitofusins in adipose tissue
… and (ii) examining the phenotype of a Mfn2R707W knock-in mouse model. Wild-type (WT), Mfn1-/-, and Mfn2-/-, mouse embryonic fibroblasts (MEFs) were differentiated into mature adipocytes. Despite the disruption of their mitochondrial morphology, Mfn1-/- MEFs manifested a marked increase in …
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Analysing The Effects Of Loss Of Sin3 In Drosophila Melanogaster
… lethality occurring due to ubiquitous knock down of SIN3 is hypothesized to be to the result of defects in cell proliferation. Conditional knock down of SIN3 in the wing discs results in a curly wing phenotype in the adult fly. These wings are smaller and have fewer cells resulting from …
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Histone Demethylase LSD1 Restricts the Size of the Germline Stem Cell Niche in Drosophila Ovaries
… formation. Clonal analysis, cell-type specific knock down and rescue experiments demonstrate that Lsd1 functions within the escort cells that reside immediately adjacent to cap cells (niche). Loss of Lsd1 causes the escort cells to adopt an intermediate fate expressing both escort cell and cap …
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A molecular genetic analysis of Crohn’s disease susceptibility loci in psoriasis
… in CD4+ and CD19+ lymphocytes. Stable CDKAL1 knock-down cell lines generated by sh-RNA lentiviral transduction were also analysed. This showed that CDKAL1 silencing results in reduced cell proliferation and altered cell cycle progression. Transcription profiling of the knock- down cells …
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The molecular mechanism of action of the antiangiogenic natural product, cremastranone
… Partial loss of FECH, using a siRNA-based knock down approach, decreased retinal angiogenesis both in vitro and in vivo in mouse models. Knock down of FECH decreased the expression levels of key proangiogenic proteins HIF-1α, eNOS, and VEGFR2. This work suggests that ferrochelatase plays an …
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