Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 47 for “"kinetochores"”.
-
Molecular Mechanisms of Mitotic Checkpoint Complex Assembly onto Kinetochores
During metaphase, in response to improper kinetochore-microtubule attachments, the spindle assembly checkpoint (SAC), activates the mitotic checkpoint complex (MCC), to inhibit the E3 ubiquitin ligase, the anaphase-promoting complex/cyclosome (APC/C). Inhibition of the APC/C then delays premature …
-
Regulation of chromosome attachment and dynamics by Saccharomyces cerevisiae kinetochores
Kinetochores are large, multi-protein complexes that bind centromeric DNA to the microtubules of the mitotic spindle and mediate chromosome movement throughout the cell cycle. The proteins that regulate both force generation at kinetochores as well as and the cell-cycle-dependent changes in …
-
Molecular analysis of kinetochore-microtubule attachment in budding yeast
Kinetochores bind to microtubules and are responsible for chromosome segregation and the accurate transmission of genetic information during cell division. Kinetochores are DNA-protein complexes that assemble on centromeric DNA sequences. In budding yeast, the kinetochores consist of approximately …
-
Investigating the Role of Inner Kinetochore Higher-Order Assembly in Kinetochore Function
… the cell builds protein complexes called “kinetochores” on regions of the chromosomes known as “centromeres.” The inner kinetochore resides on chromosomal DNA throughout the cell cycle. As mitosis approaches, post-translational modifications trigger recruitment of the outer kinetochore, …
-
Meiotic regulation of cyclin-dependent kinases
… segregation pattern. Premature interactions of kinetochores with microtubules transform meiosis I into a mitosis-like division by disrupting two key meiosis I events: coorientation of sister kinetochores and protection of centromeric cohesin removal from chromosomes. Furthermore we find that …
-
Generating the Spindle Assembly Checkpoint Signal at the Kinetochore
… senses the lack of tension/attachment on the kinetochores and consequently blocks anaphase onset by inhibiting an E3 ubiquitin ligase called anaphase-promoting complex. The roles of two kinases- BubR1 and Mps1, implicated in spindle assembly checkpoint were investigated. A checkpoint complex …
-
Generating the Spindle Assembly Checkpoint Signal at the Kinetochore
… senses the lack of tension/attachment on the kinetochores and consequently blocks anaphase onset by inhibiting an E3 ubiquitin ligase called anaphase-promoting complex. The roles of two kinases- BubR1 and Mps1, implicated in spindle assembly checkpoint were investigated. A checkpoint complex …
-
Maintenance of Genomic Integrity by RSF1 Chromatin Remodeler
… 7 III. RESULTS 9 A. RSF1 localizes at mitotic kinetochores and directly interacts with PLK1 9 B. RSF1 plays a crucial role in PLK1 deposition and activation at the mitotic kinetochores 16 C. Phosphorylation of RSF1 at Ser1375 by CDK1 is necessary and sufficient for PLK1 docking to kinetochores …
-
STUDIES IN OOCYTES FROM THREE MAMMALIAN SPECIES DEMONSTRATE THAT MEIOTIC KINETOCHORES ARE COMPOSED OF PREVIOUSLY UNIDENTIFIED SUBDOMAINS AND REVEAL TWO NOVEL MECHANISMS BEHIND THE MATERNAL-AGE EFFECT IN HUMANS
… human oocytes challenge the paradigm that sister kinetochores are fully fused. Instead, I demonstrated that sister kinetochores disjoin as women get older, which promoted erroneous kinetochore-microtubule attachments. This in turn allowed chromosomes to rotate on the spindle and provided a …
-
Analyzing Sumo-2/3 Modification In Regulation Of Breast Cancer Progression And Mitotic Chromosome Segregation
… arrest and mislocalization of CENP-E from kinetochores. CENP-E has a SUMO interacting motif required for its kinetochore localization. We found that these mitotic defects in cells with inhibition of SUMOylation can be rescued by overexpressing Nuf2-SUMO2-SUMO2-SUMO2 fusion proteins, but not …
-
Molecular mechanisms of kinetochore microtubule attachment
… complex localizes preferentially to kinetochores of bioriented sister chromatids. Localization of the Astrin-SKAP-LC8 complex to kinetochores is controlled by a key regulator of kinetochore-microtubule attachments, Aurora B kinase. The Astrin-SKAP-LC8 complex is essential for mitotic …
-
Kinetochore-Microtubule interface: analyses of Ndc80-Dam1 Interactions
… can only occur when microtubules capture kinetochores and align sister chromatids on the mitotic spindle. Initially kinetochores interact with the lateral surface of a microtubule (lateral attachment), which is subsequently converted to an end-on attachment when the tip of a shrinking …
-
The role of ZW10 and its binding partners in chromosome segregation
Kinetochores are proteinacious structures that assemble on centromeric DNA and fulfill several important functions during chromosome segregation. They attach chromosomes to microtubules of the mitotic spindle in a bipolar fashion and monitor the state of the kinetochore-microtubule attachment. In …
-
Regulation of microtubule attachment at the kinetochore and cell cortex
… a successful mitosis, including the chromosomes, kinetochores, centrosomes, and the microtubule cytoskeleton that forms the mitotic spindle. To assemble the mitotic spindle, the two centrosomes radially nucleate dynamic microtubules that make important connections with kinetochores and the cell …
-
Dissecting the Spatiotemporal Regulation of Yeast Kinetochore Interactions
Kinetochores are conserved protein complexes that bind the centromeres in replicated chromosomes to the mitotic spindle and then direct their segregation. To better comprehend Saccharomyces cerevisiae kinetochore function, we investigated the phospho-regulated dynamic interaction between the …
-
Regulation of the spindle checkpoint by Mad2 binding proteins
… proteins localize to unattached or maloriented kinetochores in mitosis and generate a signal that prevents dissolution of sister chromatid cohesion. Checkpoint signaling requires binding of Mad2 to the checkpoint protein Madl and Cdc20, a subunit of the Anaphase Promoting Complex. We have …
-
Safeguard of Mitosis: The Spindle Checkpoint
… and other human diseases. In presence of kinetochores that are not attached or improperly attached to microtubules, the checkpoint signals to assemble the mitotic checkpoint complex (MCC), which consists of BubR1-Bub3, Mad2, and Cdc20. The diffusible MCC inhibits the ubiquitin ligase …
-
Investigating mitotic vulnerabilities that arise upon oncogenic cell transformation
… and much of this regulation is centered around kinetochores. Kinetochores are large proteinaceous structures built upon centromeric heterochromatin that must form stable, load-bearing attachments to microtubules (MTs) emanating from the spindle poles. Failure to undergo high-fidelity cell …
Page 1 of 3