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Showing 1 to 20 of 25 for “"kinesin-1"”.
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Kinesin-1 mechanical flexibility and motor cooperation
Conventional kinesin (kinesin-1) transports membrane-bounded cargos such as mitochondria and vesicles along microtubules. In vivo it is likely that several kinesins move a single organelle and it is important that they operate in a coordinated fashion so that they do not interfere with each other. …
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Force studies of multiple kinesin-1 and EG5 molecular motors
Kinesin-1 and Eg5 are molecular motors that are essential for eukaryotic cell growth and development. Kinesin-1 motors transport intracellular cargo along the microtubule cytoskeleton through and against the dense viscoelastic cytoplasm. Eg5 motors mediate proper mitotic, microtubule spindle …
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How Kinesin-1 Deals With Roadblocks: Biophysical Description and Nanotechnological Application
… on biotinylated microtubules, it was shown that kinesin-1 motors first stop and then detach when they encounter a streptavidin obstacle on their path along the microtubule. Consequently, the deceleration of streptavidin coated microtubules in gliding assays could be attributed to an obstruction …
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Studies of Huntington's disease associated motor domain phosphorylation of kinesin-1
… conserved residue found in all three isoforms of kinesin-1 in impaired axonal transport associated with Huntington’s disease. Phosphorylation adds both negative charge and bulk to a protein. The mechanism by which S175 modification is related to impaired transport is not very well understood. It …
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Identification of a Novel Link between the Motor Proteins Dynein and Kinesin-1
The motor proteins dynein and kinesin are fascinating biological machines which, like vehicles in a city, move various cellular cargoes along cytoskeletal microtubules (MT). In filamentous fungi, these motors are important for the hyphal growth, which is characterized by localized extension at the …
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Gates and Latches: the mechanochemistry of ATP binding in kinesin 1 and Eg5
… cargo transport along microtubule filaments by Kinesin 1. A gating mechanism is needed to coordinate ATP binding to the two catalytic domains within Kinesin 1 in order to prevent simultaneous binding, hydrolysis and detachment of both heads which could prematurely terminate cargo transport. The …
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Engineered Cytoskeletal Arrays Reveal Mechanisms Of Membrane Transport And Tubulation
… between a processive, microtubule-based motor, kinesin-1, and a non-processive, actin filament-based motor, Myo1c, in a simplified environment with increasing physiological complexity. First, we examined the interplay between purified motors attached to a membrane-coated bead at individual actin …
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Microtubule Motors Drive Nuclear Dynamics and Positioning in Developing Skeletal Muscle Cells
… are driven by the microtubule motor proteins, kinesin-1 and cytoplasmic dynein. Depletion of the plus-end directed motor kinesin-1 abolishes nuclear rotation and significantly inhibits nuclear translocation, resulting in the abnormal aggregation of nuclei near the midline of the myotube. Loss …
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Investigating the effect of amyotrophic lateral sclerosis-associated mutant vesicle-associated membrane protein B on axonal transport
… by the microtubule-based molecular motor kinesin-1. Attachment of kinesin-1 to mitochondria involves the outer mitchondrial membrane protein Rho GTPase-1 (Miro1) which acts as a sensor for cytosolic calcium levels ([Ca2]c); elevated [Ca2+]c disrupts mitochondrial transport via an effect on …
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Messenger Ribonucleoprotein (Mrnp) Complexes: Proteomic and Functional Analysis
… with both microtubule- and actin-based motors, kinesin-1 and myosin Va. In order to unambiguously identify the detailed molecular mechanisms for RNA transport, we developed an in vivo functional assay and went on to provide evidence that dFMRP and associated mRNAs are actively transported by …
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Characterization and cooperative dynamics of kinesin motor proteins in intracellular transport
… a modular plasmid toolkit for controlled kinesin expression and purification, and I created a novel DNA origami scaffold that allows motors to be positioned with defined stoichiometry and geometry on a rigid cargo-like platform. Together, these advances provided a programmable experimental …
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Viral and cellular proteins involved in vaccinia virus egress
… viral proteins A36, F12 and E2 together with the kinesin-1 microtubule motor protein are thought to be involved in IEV egress from the site of wrapping to the cell periphery, although the exact mechanism of movement is unclear. Until recently, A36 was the only known protein to interact with the …
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Structural Basis for Coordination in Dimeric Kinesin
Kinesin-1 (conventional kinesin) is a protein motor that carries organelles and vesicle cargo along its microtubule track. The two catalytic heads of Kinesin-1 are linked to function as a highly processive "molecular walker'' that can take hundreds of steps before falling off the track. A key …
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Theoretical Studies of Protein-Protein Interactions
… survival and differentiation, and motor protein kinesin-1. First part of this thesis is devoted to a study of ERK2-EtsΔ138 system using variety of techniques from theoretical methods, e.g. First Passage Processes method, to Molecular Dynamics and Brownian Dynamics simulations. We first gain …
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Cooperative behavior of motor proteins
The cytoskeletal motor protein kinesin-1 (conventional kinesin) is the fast carrier for intracellular cargo transport along microtubules. So far most studies aimed at investigating the transport properties of individual motor molecules. However, the transport in cells usually involves the …
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The interconnected roles of spastin and protrudin in endosomal sorting
… its coiled coil domain previously implicated in kinesin-1 interaction. Tug-of-war between kinesin and dynein microtubule motors has been shown to be essential for endosomal tubule fission and I propose that protrudin functions in this process by promoting endosome interactions with kinesin-1, …
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Force studies of intracellular transport
Cytoskeletal motors like kinesin-1 and dynein are necessary for intracellular transport and a variety of other functions in the cell. They have been well characterized in simplified, single-motor, in vitro systems, but less is known about their mechanical properties in vivo, in a more complex, …
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Kinesin's force generation mechanism : study and practical application
Chimeric kinesin 1 (KHC) / kinesin 5 (Eg5) constructs were used to study the force generation mechanism of the motor protein. The kinesin family of proteins walk along microtubules to carry cargo or pull microtubules along each other and do so by hydrolyzing a single ATP per 8nm step. The mechanism …
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Patterning planar surfaces with motor proteins: Towards spatial control over motile microtubules
… areas. To confirm the successful patterning of kinesin-1 molecules and their functionality microtubule-based gliding motility assays were performed. It was shown that the microtubules bind to the patterned kinesin-1 molecules and are transported exclusively in the patterned areas. While the …
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Negative interference in systems of coupled kinesin: A study of self-assembling complexes with defined structure
… that allows structurally-defined complexes of kinesin-1 to be created. This approach also provides synthetic control over intermotor spacing and the elasticity of the mechanical motor linkages to rigorously characterize the effects of system structure on the interactions of exactly two motors. …
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