Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 22 for “"kinase/phosphatase"”.
-
MAP kinase phosphatase-2 in vascular smooth cell muscle function
… occurs involves ligand-induced activation of MAP kinase signalling (Schad et al., 2011). MAP kinases have been noteworthy but troublesome targets in cardiovascular therapeutics; thus exploration of targeting endogenous regulatory dual specificity proteins namely MAP kinase phosphatases has been …
-
REGULATION OF INFLAMMATION AND METABOLISM BY INTERFERON REGULATORY FACTOR 3 AND MAP KINASE PHOSPHATASE 5
… INTERFERON REGULATORY FACTOR 3 (IRF3) AND MAPK PHOSPHATASE 5 (MKP5) PLAY IMPORTANT ROLES IN OBESITY-RELATED METABOLIC DISEASES. WE FOUND THAT IRF3 IS A NEGATIVE REGULATOR OF ADIPOGENESIS AND ADIPOSE TISSUE INFLAMMATION THEREBY TO INHIBIT THE DEVELOPMENT OF OBESITY AND TYPE 2 DIABETES. MICE …
-
Characterization of the Components of Carbon Catabolite Repression in Clostridium perfringens
… availability. In Gram-positive bacteria, the HPr kinase/phosphatase is responsible for this nutritional sensing by phosphorylating or dephosphorylating the serine-46 residue of HPr. HPr-Ser-P then forms a complex with the transcriptional regulator CcpA to regulate transcription. We were able to …
-
A systems approach to engineering cancer nanotechnologies
… and reversible sensing of antagonistic kinase/phosphatase enzyme pairs.
-
Angiotensin-(1-7): A Peptide Hormone Inhibitor for the Treatment of Breast Cancer Targeting the Tumor Microenvironment
… increased the mitogen-activated protein (MAP) kinase phosphatase DUSP1 in tumor-associated fibroblasts, by 252 ± 29% compared with untreated cells, with an associated 50% decrease in activities of the MAP kinases ERK1 and ERK2 [1.13 ± 0.23 relative density units (RDU) in untreated cells …
-
Biochemical and Structures Studies of tRNA Modificaton and Repair Enzymes
… operon. Pnkp has previously been shown to have kinase, phosphatase and adenylyltransferase activity. Therefore Pnkp/Hen1 might be involved in RNA repair. I cloned and expressed both proteins with a pETDuet vector. Pnkp and bHen1 form a heterotetramer in gel filtration column. Purfied Pnkp/bHEN1 …
-
Biochemical and Structural Studies of RNA Modification and Repair
… bacterial species. Pnkp has been shown to have kinase, phosphatase, and adenylyltransferase activities, which are hallmarks of RNA repair. However, Pnkp alone is not sufficient for ligation. Pnkp/Hen1 was shown to form a tetramer during purification in size exclusion gel filtration. I carried …
-
ANGIOTENSIN-(1-7): A NOVEL SMALL MOLECULE FOR THE TARGETED TREATMENT OF TRIPLE NEGATIVE BREAST CANCER
… attenuates cell growth. Activity of the MAP kinases ERK1 and ERK2 was reduced in tumors of Ang-(1-7)-treated mice which was associated with a receptor-mediated increase in the MAP kinase phosphatase DUSP1 (1.01 ± 0.1 relative gene expression in tumors from saline-treated mice compared to 1.78 …
-
Post-translational control of <em>Bacillus subtilis</em> biofilm formation
… These proteins are PtkA, a bacterial tyrosine kinase; TkmA, the cognate modulator of PtkA; and PtpZ, a bacterial tyrosine phosphatase. By introducing amino acid point mutations within the catalytic domains of PtkA and PtpZ it was identified that the kinase phosphatase activities, respectively, …
-
Discovery and characterization of novel RNA repair systems
… the bacterial Pnkp has been shown to possess kinase, phosphatase for RNA healing and all the signature motifs of a RNA ligase, it alone is not able to carry out RNA repair. In our study, we crystallized an active RNA ligase consisting of the C-terminal half of Pnkp (Pnkp-C) and the N-terminal …
-
A Study on the Effect of Peroxisome Proliferator-Activated Receptors (PPARs) Activation in Cultured Rat Brain Glial Cells
… on AP-1/SP-1 were mediated by Jun N-terminal kinase (JNK) and MAP kinase phosphatase-1 (MKP-1), upstream molecules of them. Taken together, these results show that signaling of MCP-1 expression differ from stimulus, such as LPS and IFN-？, and their production was differentially regulated by …
-
Illuminating Organellar and Molecular Organization of the Endomembrane System
… reticulum by the phosphatidylinositol 3-kinase MavQ secreted by Legionella pneumophila. L. pneumophila exploits the host endoplasmic reticulum membrane to form a Legionella-containing vacuole for intracellular replication. I reveal that MavQ generates phosphatidylinositol 3-phosphate on …
-
Immunological control of toxoplasma gondii infection
… expression is negatively regulated by Map Kinase Phosphatase-2 (MKP-2), the second major aim of the project was to study the effect of MKP-2 deficiency on T. gondii infection. MKP-2-/- mice were found to be more susceptible to infection with increased parasite growth and increased mortality …
-
The Differential Contribution of Striatonigral and Striatopallidal Neurons in Mediating Responses to Therapeutic Agents and Drugs of Abuse: A Dual Role for DARPP-32
… consequences. DARPP-32 is a dual function kinase/phosphatase inhibitor which has been shown to be a key mediator of signaling in both striatonigral and triatopallidal neurons. A variety of therapeutic agents and drugs of abuse can affect the phosphorylation of DARPP-32. Psychostimulants …
-
Induction and Maintenance of Synaptic Plasticity
… involving calcium/calmodulin-dependent protein kinase II (CaMKII) and its regulating protein signaling cascade has been hypothesized to durably maintain the synaptic state in form of a bistable switch. Furthermore, it has been shown experimentally that CaMKII and associated proteins such as …
-
The effect of exercise on spatial learning and hippocampal proteins in maternally separated adult rats
… phospho-extracellular signal-regulated protein kinase (pERK) in the hippocampus of non-separated-exercised rats. In the current study, the same MS technique and three-week voluntary exercise regimen were tested to determine their effect on spatial learning in young adult Sprague-Dawley (SD) …
-
Mitotic Exit: Thresholds and Targets
<p>Cyclin dependent kinases (CDKs) are at the heart of the cell cycle. Throughout the cycle, these complexes modify many proteins, changing various aspects of their regulation (stability, localization, etc.). As cells exit mitosis, the CDK that has driven many of the cell cycle processes is …
-
Effect of Scutellaria Baicalensis on Reduction of Radiation/chemotherapy induced Inflammation
… expression of Mitogen Activated Protein Kinase Phosphatase-1 (MKP-1), possibly leading to inhibiting the inflammation. Furthermore, S decreased slightly the NFκB retention within nucleus of the LPS stimulated RAW cells. There was no significant effect to reduce the inflammatory severity …
-
Eliminating Acute Myeloid Leukemia Stem Cells by Targeting the Niche Microenviromnent: Co-Inhibition of TNF/IL1- JNK and NF-κb
… to <60 min through dephosphorylation by Map Kinase Phosphatase 5, and also by upregulating JNK's nuclear target c-Jun. Such short duration of JNK activation correlates to activation of JNK's nuclear targets without activating the death signal.</p><p>I determined that concurrent inhibition of …
Page 1 of 2