Global ETD Search
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Showing 1 to 15 of 15 for “"karyopherins"”.
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The Regulation of Cellular Localization of Both Active and Inactive ERK1/2
… of ERK2 mutants, the interactions of ERK2 with karyopherins β1 and β2, and explored the roles of cytoskeletal elements, motor proteins and scaffolding complexes in the localization of ERK1/2. Mutation or dysregulation of the ERK1/2 signaling cascade has been identified in a host of diseases from …
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Biochemical Analysis of the Protein-Protein Interactions Involved in Karyopherin-Mediated Transport Across the Nuclear Pore Complex
… must be aided across by shuttle proteins (karyopherins, or Kaps). Kap-mediated transport involves FG-nups, a family of NPC proteins. While much has been learned about the mechanism of nucleocytoplasmic transport, many details are still unknown; perhaps among the most important missing …
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Characterization of Nuclear Localization Signals of Karyopherin-Mediated Nuclear Import
… of the NLSs in NXF1 abolished binding to the Karyopherins, mislocalized NXF1 to the cytoplasm and significantly compromised its mRNA export function. Sequence examination of NXF1 from divergent eukaryotes and the interactions of NXF1 homologs with various Karyopherins have revealed the …
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Studies on the interaction of HSV-1 multifunctional protein ICP27 with the nuclear pore complex proteins
… mRNA bound proteins and transport receptors, karyopherins. To further explore the transport requirements for ICP27 and viral mRNA export, we investigated its direct interaction with nucleoporins and show that ICP27 interacts directly with nucleoporins tested, in vitro and in wild type and …
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Shield and Shuttle: Importins as Protective Chaperones and the Nuclear Transport Mechanism of H2A-H2B
… to the nucleus is carried out by a sub-class of Karyopherins called Importins. It was hypothesized back in the early 2000s that importins could have dual functions as 1) nuclear importers and 2) molecular chaperones. This thought stems back to characteristics of importins: they are negatively …
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Nucleocytoplasmic Localization of MAPKs
… events require carrier proteins known as karyopherins (importins and exportins), ERK2 enters the nucleus of permeabilized cells even if these carrier proteins are missing. This is explained by direct binding to proteins in the nuclear pore complex (NPC). Similar to ERK2 targets, NPC …
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Structural and Biochmical Studies of Nuclear Import Factor Karyopherin Beta-2
… significant and may be conserved in other import Karyopherins.
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A Proteomic Approach to Elucidating the Function of Picornavirus 2A Protease
… accumulation of proteins normally transported by Karyopherins that interact with Nup98 FG motifs. We developed fluorescent transport reporters with various nuclear import and export signals to elucidate their transport mechanism and dependence on Nup98. Nuclear localization signals promiscuously …
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Kap-[beta]2 Recognition of Cargoes Involved in Neurodegenerative/Neurodevelopmental Diseases
Karyopherin β2 is a nuclear-import receptor that transports many RNA-binding proteins into the nucleus by recognizing within the cargoes a unique nuclear-localization signal known as the PY-NLS. Several of Kapβ2 cargoes are mutated in neurological diseases. Two such proteins of interest to my …
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Structural and Biochemical Studies of Multiple Importin-Histone Interactions
Multiple Importins can bind the N-terminal tails of histones H3 and H4, and import them into the nucleus to be assembled into the nucleosomes. However, it is not known what sequence elements in the histone tails are recognized by each of the Importins. Through structural and quantitative …
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Recognition Mechanisms of Nuclear Localization and Export Signals
The transport of proteins between the nucleus and cytoplasm of cells is mediated by the Karyopherin beta family of proteins. Karyopherin betas recognize their substrates through either a nuclear localization or export signal depending on the direction of transport. Even though there are ten yeast …
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Characterization of the Beta-Karyopherin Protein Ipo9 During Germ Cell Differentiation
Germ cells participate in the most fascinating process in nature, the fusion of two cells that ultimately give rise to an entire organism. Errors in germ cell development directly affect the fertility of the parent organism and/or the health of their offspring. A myriad of molecules such as …
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Nuclear Export Signal Recognition by CRM1 Carrying the Oncogenic E571K Mutation and Structure-Based NES Prediction
Nuclear-cytoplasmic trafficking is an essential cellular process in eukaryotes that maintains and regulates the spatial distribution of key cellular processes in the nucleus and the cytoplasm. CRM1 is the major nuclear export mediator, which facilitates nuclear export of hundreds of protein and RNA …
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Nuclear Export Receptor CRM1 Recognizes Nuclear Export Signals with Diverse Conformations
The Chromosome Region of Maintenance 1 or CRM1 protein facilitates export of hundreds of proteins and RNA molecules from eukaryotic cell nuclei. CRM1 recognizes its protein cargoes by their 8-15 residues-long nuclear export signals or NESs, which bind to a hydrophobic groove in CRM1. NESs are …
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Identification of Oncogenic KRAS-Associated Vulnerabilities in Non-Small Cell Lung Cancer
Activating mutations in KRAS are frequently involved in the pathogenesis of non-small cell lung cancer (NSCLC), the disease responsible for the most cancer-related deaths in the US. Despite intensive efforts to develop drugs that directly interfere with KRAS activity over the past decade, no …