Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"karyopherin"”.
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Karyopherin alpha subtypes and porcine early stage embryo development
… cellular events during embryogenesis. The karyopherin α/β heterodimer is an intracellular nuclear trafficking system that mediates nuclear import of proteins that bear classical nuclear localization signals (NLSs). Seven karyopherin α subtypes have been identified in the domestic pig, and …
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Investigating Karyopherin B1: small molecule interactions for cancer therapy
… the eukaryotic nuclear importer protein Karyopherin B1 (KpnB1), to be up-regulated in different cancer cell lines, including cervical and oesophageal as well as transformed cells. Inhibition of KpnB1 in these cells using small interfering RNA (siRNA) resulted in significant cancer cell …
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Characterization of Nuclear Localization Signals of Karyopherin-Mediated Nuclear Import
Nucleocytoplasmic transport is mediated by Karyopherin beta (Kap beta) proteins in a Ran-dependent manner. Ten import Kap betas recognize their cargos through the nuclear localization signals (NLSs) and carry them into the nucleus. Recent structural and biochemical work on Kap beta2 (or …
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Structural and Biochmical Studies of Nuclear Import Factor Karyopherin Beta-2
Karyopherinbeta (Kapbeta ) proteins recognize nuclear localization and export signals (NLS/NES) and mediate the transport of macromolecules across the nuclear envelope, a process regulated by Ran GTPase through its nucleotide cycle. Diversity and few number of available signal sequences recognized …
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Characterization of the Beta-Karyopherin Protein Ipo9 During Germ Cell Differentiation
… through nuclear trafficking machinery. The karyopherin family of proteins is responsible for nuclear import and export. The contribution of nuclear trafficking during gametogenesis is not well understood. Here we demonstrated that the well conserved β-karyopherin Importin-9 (Ipo9) is …
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The role of Karyopherin β1 in the nuclear import of HIV-1 proteins
Includes abstract. Includes bibliographical references.
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Modulating the expression and activity of the nuclear import protein, Karyopherin β1, in cancer cells
… cancer compared to normal patient tissue, found Karyopherin β1 (Kpnβ1), the primary nuclear import protein, to be significantly overexpressed in cervical cancer tissue. Further studies showed that inhibition of Kpnβ1 expression by siRNA resulted in cancer cell death, while non-cancer cells were …
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Recognition Mechanisms of Nuclear Localization and Export Signals
… and cytoplasm of cells is mediated by the Karyopherin beta family of proteins. Karyopherin betas recognize their substrates through either a nuclear localization or export signal depending on the direction of transport. Even though there are ten yeast import Karyopherin betas, for the past …
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Biochemical Analysis of the Protein-Protein Interactions Involved in Karyopherin-Mediated Transport Across the Nuclear Pore Complex
… must be aided across by shuttle proteins (karyopherins, or Kaps). Kap-mediated transport involves FG-nups, a family of NPC proteins. While much has been learned about the mechanism of nucleocytoplasmic transport, many details are still unknown; perhaps among the most important missing …
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Characterising the anticancer effects of a small molecule with potential to inhibit nuclear import via karyopherin beta1
The Karyopherin superfamily is a group of soluble transport proteins which are involved in nuclear-cytoplasmic trafficking. Studies have shown the involvement of Karyopherin proteins in nuclear pore assembly, nuclear membrane assembly and DNA replication. Since all these cell regulatory functions …
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Mechanism of Nuclear Protein Import and Export an In Vitro Approach
… docking of the transport cargo at the NPC via a karyopherin, release of the cargol karyopherin complex, and subsequent recycling of the transport factors for another round of function. However, although the proteins of the general transport machinery involved are the same for nuclear protein …
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Analyse der differentiellen Expression von Transportfaktoren und deren Funktion bei dem nukleocytoplasmatischen Transport von TFIIIA
… 7S RNP Komplexes.Mit den in Xenopus laevis neuen Karyopherin alpha Varianten konnte gezeigt werden, dass TFIIIA-delta-56 bei Verwendung von frisch erstellten Karyopherin alpha Fusionsproteinen in vitro spezifisch an alpha1 und alpha2 binden kann. Beide Varianten zeigen in RT-PCR und whole mount" …
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Das Protein La/SS-B: Vom Autoantigen zur Zielstruktur für die Immuntherapie
… wird durch die klassischen Transportmoleküle Karyopherin-α und Karyopherin–β vermittelt. Für den Shuttlingprozess muss das Protein auch wieder aus dem Kern exportiert werden. Da es kontroverse Daten bezüglich eines Crm1-abhängigen Kernexports gab, wurde das Shuttlingverhalten von …
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Tap /Nxf1 N -Terminal Domain Mediates Homotypic Complex Assembly and Is Necessary for Nuclear RNA Export
… that the NXF family is unique from the Karyopherin transport receptor family and that RNA export functions independently of the nuclear Ran-GTP gradient. Until now the mechanistic basis for Tap-mediated export has remained elusive; the work herein provides analysis of Tap homotypic …
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Kap-[beta]2 Recognition of Cargoes Involved in Neurodegenerative/Neurodevelopmental Diseases
Karyopherin β2 is a nuclear-import receptor that transports many RNA-binding proteins into the nucleus by recognizing within the cargoes a unique nuclear-localization signal known as the PY-NLS. Several of Kapβ2 cargoes are mutated in neurological diseases. Two such proteins of interest to my …
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Biological Functions of Intracellular Hepatitis B e Antigen
… translocation of p-STAT1 by interacting with karyopherin α1, indicating that the cytoplasmic p22 may impede JAK-STAT signaling to help the virus evade host innate immune response and cause resistance to IFN therapy in patients. Additionally, nuclear p22 and nuclear core were found to interact …
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Stress-Induced Targeting of Molecular Chaperones In The Yeast Saccharomyces Cerevisiae
… of quiescent Saccharomyces cerevisiae cells in a karyopherin-dependent manner. I isolated nuclear accumulation- defective HSP82 mutant alleles to probe the nature of this targeting event and identified a mutant with a single amino acid substitution (I578F) sufficient to prevent nuclear …
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Investigating the effect of a small molecule, Inhibitor of Nuclear Import (INI-43) as a pan-cancer treatment
Karyopherin beta 1 (KPNβ1) is a nuclear import protein that transports cargo molecules from the cytoplasm into the nucleus and plays an important role in other cellular processes such as mitosis and mitotic spindle assembly, DNA replication and cell cycle progression. Previous studies in our …
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The effect of inhibiting KPNB1-mediated nuclear import on cancer cell biology and inflammatory transcription factor signalling
… others identified the nuclear import protein, Karyopherin β1 (KPNB1), to be overexpressed in various cancers and that inhibiting its expression blocks the proliferation of cancer cells. However, little is known about the potential role of KPNB1 in other cancer cell phenotypes and inflammatory …
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Investigating the nuclear import protein KPn߀1 as a cancer therapeutic target
… expression of the nuclear import protein Karyopherin Beta 1 (Kpnβ1) in various cancers. The inhibition of Kpnβ1 by siRNA silencing inhibited the proliferation of cancer cells and induced cell death via apoptosis while having little effect on non-cancer cells. These findings suggested that …
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