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Showing 1 to 9 of 9 for “"k lactis"”.
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Bioengineering the Expression of Active Recombinant Human Cathepsin G, Enteropeptidase, Neutrophil Elastase, and C-Reactive Protein in Yeast
<p>The yeasts Pichia pastoris and Kluyveromyces lactis were used to express several recombinant human proteins for further biochemical characterization. Two substitution variants of recombinant human enteropeptidase light chain (rhEPL) were engineered to modify the extended substrate specificity of …
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Obtenção de linhagens de Kluyveromyces lactis recombinantes produtoras do imunógeno SBm 7462 contra o carrapato Riphicephalus (Boophilus) microplus (Canestrini, 1887)
… a obtenção de linhagens de Kluyveromyces lactis recombinantes produtoras do referido peptídeo. Dois vetores de expressão integrativos foram construídos a partir do vetor pKLAC1: pKLAC1-seq1, contendo a seqüência 1 constituída por três cópias da seqüência de nucleotídeos que codifica para o …
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Investigação das etapas limitantes durante a síntese de Ácido Hialurônico em Kluyveromyces lactis
… até 74 g/L. Uma cepa da levedura luyveromyces lactis, denominada cepa BAP, foi construída visando produzir AH através da inserção dos genes hasA (codificante da enzima Hialuronan Sintase) e hasB (codificante da enzima UDP-Glicose Desidrogenase). Porém, após cultivo em biorreator foi alcançada …
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Functional and evolutionary analysis of sugar transporters in Kluyveromyces marxianus
… shown to encode a galactose transporter in K. lactis, we tested whether any of the LAC12 genes from K. marxianus encoded a similar function. Heterologous expression of these genes revealed that three out of the four copies encoded galactose transporters – no function was identified for LAC12-3. …
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The influence of yeasts on the aroma of Stilton cheese
… decreased as the concentration of Kluyveromyces lactis, inoculated into models containing P. roqueforti, decreased. Sensory analysis indicated the effects of Y. lipoltyica and K. lactis on aroma profiles observed instrumentally may not be perceivable by humans.<br/><br/>The results of this study …
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Structural and Functional Characterization of the Putative Pineal ATPase PINA
… putative isoforms of hu-man PINA homologue in K. lactis expression system. We have established a purification proce-dure that enabled us to get active enzymes and study their ATPase activity. This is the first suc-cessful purification of the active full-length human ATP7B reported. The activity of …
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Immobilisierung von BPA-Bindeproteinen an der Oberfläche von Hefezellen
… protein at the cell surface. KYE1 from K. lactis was chosen as BPA binding protein because it is highly homologous to the phenol binding protein OYE from S. cerevisiae and the steroid binding protein EBP1 from C. albicans. To display KYE1 at the cell surface it had to be fused with the …
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Production and characterisation of yeast cell wall preparations with binding activity against Salmonella and Escherichia coli.
… using the developed assay. S. cerevisiae 695, K. lactis 752, and Schiz. pombe 70572 cell wall samples had significantly higher binding activity than the control MOS used to develop the assay. The relationship between the yeast cell wall saccharide content and binding activity varied between …