Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"isotopomer"”.
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Symmetry Specific Study of Ozone Isotopomer Formation
… i.e. the simply 17O or 18O substituted ozone isotopomers could thereby be quantified. Precision in the measurement of the isotope abundance was improved to 0.3%. Systematic errors in earlier investigations were identified and minimized in the present measurements. Relative to the reaction 16O …
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Spectroscopic study of the acetylene species
… spectroscopic studies of acetylene and its HCCD isotopomer. Three different kinds of experiments have been performed: Laser-Induced Fluorescence (LIF), Dispersed Fluorescence (DF), and Stimulated Emission Pumping (SEP). Each experiment has yielded new discoveries in the vibrational and rotational …
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Development of Steady-State and Dynamic Flux Models for Broad-Scope Microbial Metabolism Analysis
… in MATLAB was developed, which allowed both mass isotopomer distribution (MID) analysis and metabolic flux calculations. Several bacterial templates with diverse substrate utilizations were included in this platform to facilitate 13C-MFA model construction. The corrected MID data and flux profiles …
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Comprehensive analysis of metabolic pathways through the combined use of multiple isotopic tracers
… and GC/MS measurements, is the large number of isotopomer equations that need to be solved. This restriction reduces the ability of MFA to fully utilize the power of multiple isotopic tracers in elucidating the physiology of complex biological networks. Here, we present a novel framework for …
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Systems Metabolic Engineering of Microbial Cell Factories for the Synthesis of Value-added Chemicals
… oxygen availability. In addition, the use of 13C-isotopomer analysis method was proposed for the accurate determination of product yields in engineered microbes under complex cultivation conditions: Chapter 3).</p><p>Second, metabolic engineering of the cyanobacterium, …
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Making Nuclear Magnetic Hyperpolarization Practical through Storage in Disconnected Eigenstates
… by using the naturally occurring doubly-labeled isotopomer. I verified this method with two compounds, diphenyl acetylene (DPA) and diethyl oxylate (DEO). The former was determined to have a singlet lifetime TS = 251.40 ±3.16 s from the synthesized species, while the naturally occurring …
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Development and Application of Fluxomics Tools for Analyzing Metabolisms in Non-Model Microorganisms
… carbon metabolic routes by tracing and analyzing isotopomer labeling patterns of different metabolites after growing cells with 13C-labeled substrates. Novel pathways, including Re-type citrate synthase in tricarboxylic acid cycle and citramalate pathways as an alternate route for isoleucine …
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Reversible binding of dihydrogen to dinuclear ruthenium complexes containing chelating diphosphines
… a ¹JHD of 29.4 Hz was found for the η²-HD isotopomer, and the η²-H₂ ligand is replaceable by N₂. The reversible equilibrium conversion under H₂ of [RuCl(DPPB)₂(μ-Cl)]₂, 9, to (η²-H₂)Ru(DPPB)(μ-Cl)₃RuCl(DPPB), 9a , in CH₂Cl₂ has been studied at 0-25°C by UV-vis and ³¹P{¹H}NMR spectroscopy, …
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Metabolic flux analysis of Escherichia coli MG1655 under octanoic acid (C8) stress
… bacterial growth and proteinogenic amino acid isotopomers were measured using two-dimensional (13C, 1H) HSQC NMR spectroscopy. Notable differences of several amino acids isotopomer abundance were observed between the control condition and C8 stress condition, suggesting that the precursor nodes …
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Investigation of Gut-Derived Circulating Succinate in Metabolism and Liver Signaling
… and using a combination of germ-free mice and isotopomer tracing, we demonstrated that intestinal microbiota are not major contributors to circulating TCA cycle metabolites. Moreover, we demonstrated that endogenous succinate production is markedly higher than intestinal succinate absorption in …
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Chemistry of highly reactive group 5 and 6 transition metal compounds
… with LiAlD₄, allows for the isolation of the isotopomer W(PMe₃)₄(η²–CHDPMe₂)H, thereby providing a means to measure the rate constant for the formation of the 16-electron species [W(PMe₃)₅] from W(PMe₃)₄(η²-CH₂PMe₂)H. Chapter 2 describes the reactivity of trimethylphosphine complexes of …
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Production and utilization of phytoene, phytofluene, and lycopene tracers for bioavailability and biodistribution studies
… in the M+40 form, and 88% in the M+35 to M+40 isotopomer range. In conclusion, the hp-1 cell line is preferable to other allelic variants or the VFNT cherry cell line for labeled tomato carotenoid production. Lastly, the gerbil was utilized as a model of tomato carotenoid absorption and …
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Experimental and theoretical vibrational spectroscopic studies of diketopiperazines
… and IR spectra for C-CySH and its deuterated isotopomer are reported. The strong band at 1662 cm-1 observed in IR and at 1663 cm-1 in the Raman spectrum is assigned to the C=O stretching mode (cis amide I mode). On deuteration the cis amide I band shows a downward shift of around 54 cm-1 in …
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Study of the ligand effects on the metal-ligand bond in some new organometallic complexes using FT-Raman and -IR spectroscopy, isotopic substitution and density functional theory techniques
… shifts in the FT-Raman and –IR spectra of the isotopomer mer-[W(CO)3(dmpe)(h2-S=CD2)] confirmed our assignment. The different stereoisomers of complex mer-[W(CO)3(dmpe)(h2-S=CH2)] were investigated too, since RMN and IR-data have shown that complex mer-[W(CO)3(dmpe)(h2-S=CH2)] lead in solution …
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The Electronic and Vibrational Spectroscopy of Matrix-Isolated Phthalocyanines – Experiment and Theory
… 400 to 4000 cm-1 in solid N2, Ar, Kr and Xe. The isotopomers HDPc and D2Pc were synthesised in an attempt to resolve the conflicting assignments that currently exist in the literature for the N-H bending modes in H2Pc spectra. Comparison of the spectroscopic results obtained with isotopic …
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Microbial Regulation of Soil Greenhouse Gas Emissions in a Non-Bloat Legume Grazing System
Cattle pastures are a source of greenhouse gas (GHG) emissions, including enteric methane from ruminating cattle, carbon dioxide (CO2), and nitrous oxide (N2O) from microbial respiration of soil carbon (C) and nitrogen (N). Producers may introduce non-bloat legumes to cattle pastures to improve …