Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 51 for “"isotope-labeling"”.
-
Cell-Cycle Specific Biosynthesis of the Photosynthetic Membrane of Rhodopseudomonas Sphaeroides: Structural Implications
… electron microscopy (EM), intrinsic density and isotope labeling were used to demonstrate chromatophore fusion. Except increased ion permeability, the orientation and integrity of fused chromatophores were demonstrated to be identical to control chromatophores by freeze-fracture EM, proteolytic …
-
COMPARATIVE PROTEOMICS STUDIES OF SOLUBLE NUCLEAR PROTEINS OF DRUG SUSCEPTIBLE AND RESISTANT HUMAN BREAST CANCER MCF-7 CELLS
… Two dimensional gel electrophoresis and stable isotope labeling by amino acids in cell culture are used to conduct the study. Protein identification is acquired by peptide fingerprinting or microsequencing. Relative quantitation of the proteins is derived from gel comparisons and from ratios of …
-
Comprehensive analysis of metabolic pathways through the combined use of multiple isotopic tracers
… limitation of MFA, as carried out via stable isotope labeling and GC/MS measurements, is the large number of isotopomer equations that need to be solved. This restriction reduces the ability of MFA to fully utilize the power of multiple isotopic tracers in elucidating the physiology of complex …
-
Investigating the biosynthesis of fosfazinomycin
… producing organism and conducted stable isotope labeling experiments. The results of those studies revealed that the primary amine from aspartic acid is incorporated into the phosphonohydrazine moiety of fosfazinomycin. Further purification and spectroscopic efforts additionally …
-
Nitrogen chemistry in an urban bioretention system in Singapore
… Further study in the Balam Rain Garden using isotope labeling to more clearly delineate nitrogen fate and transport is suggested.
-
Quantitative analysis of carbon fluxes for fat biosynthesis in wild-type and IRS-1 knockout brown adipocytes
… KO) brown adipocytes were analyzed by stable-isotope labeling, GC/MS, and flux estimation. Glutamine contributed more to fatty acid synthesis than glucose in WT cells while glucose's contribution was heavier in IRS-1 KO cells. Unlike the straightforward pathway for lipogenesis from glucose, …
-
Identification of a novel metastasis enhancer, CDCP1, and analysis of its functions during melanoma metastasis
… low metastatic abilities. Using SILAC (stable isotope labeling with amino acids in culture) coupled with nano-spray tandem mass spectrometry, this work led to the discovery of C̲ub Ḏomain C̲ontaining Protein 1 (CDCP1) as one of those differentially expressed transmembrane proteins. We found …
-
Differential expression profiling of proteomes of pathogenic and commensal strains of Staphylococcus aureus using SILAC
… (MS)-based proteomic approach, known as stable isotope labeling of amino acids in cell culture (SILAC). Four commensal and pathogenic strains each were grown in the SILAC minimal media (RPMI 1640), containing light (12C) and heavy (13C) form of lysine, respectively, until early stationary growth …
-
Study of the differential compartmentation and functions of hexokinase isoforms and implications for cancer metabolism
… for cell growth and proliferation using stable isotope labeling and metabolic flux analysis. Key findings were verified in a hepatocellular carcinoma cell line using low glucose feeding or HK inhibitor. These experiments identified differential HK enzymatic activity in different cell …
-
Cell Wall/Surface Proteome of Candida albicans: its Application in Rapid Identification of Yeast Species by Mass Signature and Characterization by in vitro and in vivo Chemical Labelings
… segments of these proteins. SILAC (Stable Isotope Labeling by Amino acids in Cell Culture) based CWP quantification analyses were performed to monitor CWP accumulation level change in response to hyphae induction. Information on surface exposed peptide segments and regulation of cell …
-
DEVELOPMENT OF SINGLE-CELL MASS SPECTROMETRY TOOLS TO INVESTIGATE METABOLIC REORGANIZATION DURING EARLY EMBRYOGENESIS
… of metabolism. Chapter 4 discusses a stable-isotope labeling strategy and single-cell CE-MS to uncover metabolic pathways involved in cell differentiation. Chapter 5 details the application of our custom-built microprobe sampling technique to investigate spatial cell heterogeneity in the same …
-
Proteomic Analysis of Plasma Membrane Proteins from Drug Susceptible and Drug Resistant Breast Cancer Cell Lines
… then used in combination with metabolic stable isotope labeling to identify protein expression changes between the mitoxantrone drug susceptible and drug resistant MCF-7 breast cancer cell lines.
-
N⁶-formylation of lysine : a pathological secondary modification of proteins
… as sources of the adduct. Further, other isotope labeling studies revealed that lysine demethylation in histone proteins is not a source of N⁶-formyllysine. With regard to N⁶-formyllysine persistence in cells, our investigation of histone deacetylases revealed that despite chemical …
-
The Processing of Amyloid Precursor Protein in the Central Nervous System of Humans and Rhesus Macaques
… from CSF of participants undergoing stable isotope labeling kinetics studies. Isolated sAPP underwent proteolytic cleavage by trypsin and turnover rates of total sAPP were determined by LC-MS and compared to turnover rates of Aβ. Isolated total sAPP that underwent proteolytic cleavage by …
-
ABSOLUTE QUANTIFICATION IN SMALL PLANT RADIOTRACER STUDIES
… into account differences in the amount of radioisotope assimilated that are available for transport and allocation to the various sinks, that is, absolute CO2 uptake and photosynthetic rates are important factors in understanding the holistic physiological responses of plants to external …
-
IQGAP1 is a novel effector of gonadotropin-releasing hormone receptor signaling
… low-density plasma membranes. SILAC (stable isotope labeling by amino acids in cell culture) in combination with mass spectrometry demonstrated that GnRH, within 10 min, altered the association of 87 proteins with this plasma membrane fraction. Ontology analysis revealed that GnRH promoted an …
-
Sustainable Nanomaterials Combined with Raman Spectroscopy-based Techniques to Advance Environmental Sensing
… application presents a simple Raman-stable isotope labeling approach for the study of viral infection of bacteria. The third application involved use of SERS pH nanoprobes for measuring pH in droplets of complex matrices (e.g., DMEM cell culture media, human saliva).
-
New insights into the marine oxygen cycle from manganese oxide minerals and reactive oxygen species
… on the abundance of the three stable isotopes of O2 in the environment. In this thesis, I explore two aspects of the oxygen cycle in the past and present. First, I investigate the ability of manganese (Mn) oxide minerals to capture and retain the oxygen isotopic signature of dissolved …
-
Modulating Amyloid Beta Clearance by Altering Cellular and Whole Brain Apolipoprotein E Metabolism
… we developed a technique that couples stable isotope-labeling kinetics (SILK) with mass spectrometry. We validated this technique by demonstrating that apoE clearance is enhanced in the brains of mice overexpressing LDLR. We also applied this technique to measure apoE clearance rates in the …
-
Metabolic Diversity in Human Non-Small Cell Lung Cancer Cells
… nutrient utilization, nutrient addiction, and isotope labeling patterns following culture with 13C-glucose or 13C-glutamine. Orthogonal data sets included analysis of the genome, epigenome, transcriptome and proteome, as well as sensitivity to over 40 chemotherapeutic agents. Several cell lines …
Page 1 of 3